The olfactory structure of Korean spined loaches, Iksookimia koreensis, I. pumila, I. pacifica, I. longicorpus, I. hugowolfeldi, I. yongdokensis, were investigated by anatomical observation and statistical analysis. They share the same olfactory morphology characterized by tubular anterior and flat posterior nostrils and a rosette showing a radial arrangement of finger-like curved lamellae. However, the number of olfactory lamellae (LN) differs by species: 19-25 in I. longicorpus (with standard length, SL, 113.2±11.5 mm, mean±SD), 17-20 in I. yongdokensis (88.1±8.8 mm), 14-20 in I. hugowolfeldi (77.4±8.1 mm), 16-19 in I. koreensis (76.1±6.5 mm), 15-20 in I. pacifica (100.9±17.3mm), 15-19 in I. pumila (77.8±5.5 mm). In particular, I. koreensis (23.0±1.4%) and I. hugowolfeldi (22.4±2.4%) shows higher LN/SL ratios than do I. longicorpus (19.4±1.8%) and I. pacifica (17.5±2.0%). This comparison of LN and LN/SL ratio among species could be used as new taxonomic key of Iksookimia.