Early life exercise training and inhibition of apoLpp mRNA expression to improve age-related arrhythmias and prolong the average lifespan in Drosophila melanogaster

Abstract

Cardiovascular disease (CVD) places a heavy burden on older patients and the global healthcare system. A large body of evidence suggests that exercise training is essential in preventing and treating cardiovascular disease, but the underlying mechanisms are not well understood. Here, we used the Drosophila melanogaster animal model to study the effects of early-life exercise training (Exercise) on the aging heart and lifespan. We found in flies that age-induced arrhythmias are conserved across different genetic backgrounds. The fat body is the primary source of circulating lipoproteins in flies. Inhibition of fat body apoLpp (Drosophila apoB homolog) demonstrated that low expression of apoLpp reduced the development of arrhythmias in aged flies but did not affect average lifespan. At the same time, exercise can also reduce the expression of apoLpp mRNA in aged flies and have a protective effect on the heart, which is similar to the inhibition of apoLpp mRNA. Although treatment of UAS-apoLpp RNAi and exercise alone had no significant effect on lifespan, the combination of UAS-apoLpp RNAi and exercise extended the average lifespan of flies. Therefore, we conclude that UAS-apoLpp RNAi and exercise are sufficient to resist age-induced arrhythmias, which may be related to the decreased expression of apoLpp mRNA, and that UAS-apoLpp RNAi and exercise have a combined effect on prolonging the average lifespan.www.aging-us.com AGINGremodeling, obesity, and even sudden cardiac death, which threatens health [10][11][12]. However, there is considerable difficulty and complexity in studying cardiac aging due to long life spans and genetic redundancy in mammals. Therefore, we utilized the Drosophila model to design this experiment.