Early-Life Antibiotic-Driven Dysbiosis Leads to Dysregulated Vaccine Immune Responses in Mice

Lynn, Miriam A.; Tumes, Damon J.; Choo, Jocelyn M.; Sribnaia, Anastasia; Blake, Stephen J.; Leong, Lex E. X.; Young, Graeme P.; Marshall, Helen; Wesselingh, Steve; Rogers, Geraint B.; Lynn, David J.

doi:10.1016/j.chom.2018.04.009

Cited by 150 publications

(176 citation statements)

References 51 publications

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“…Furthermore, it has been indicated that early-life gut microbiota dysbiosis damages vaccine immune responses [49,50]. In this study, we found that early-life intervention with FMT-CS can reduce the risk of gut microbiota dysbiosis, as well as improve the antibody levels of M.hyo and PCV2.…”

Section: Early-life Intervention With Fmt-cs Promotes the Developmentmentioning

confidence: 60%

“…Potential pathogens, such as Fusobacteriaceae, Lachnospiraceae, Enterobacteriaceae, and Akkermansia, were significantly decreased in treated piglets, indicating a lower occurrence rate of intestinal dysbiosis [50]. The above results suggest that early-life intervention with FMT-CS increases the relative abundance of beneficial bacteria, while decreases that of opportunistic pathogens.…”

Section: Early Intervention With Fmt-cs Increases Beneficial Bacteriamentioning

confidence: 70%

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Early-Life Intervention Using Fecal Microbiota Combined with Probiotics Promotes Gut Microbiota Maturation, Regulates Immune System Development, and Alleviates Weaning Stress in Piglets

Xiang

Pan

et al. 2020

IJMS

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Previous studies have suggested that immune system development and weaning stress are closely related to the maturation of gut microbiota. The early-life period is a "window of opportunity" for microbial colonization, which potentially has a critical impact on the development of the immune system. Fecal microbiota transplantation (FMT) and probiotics are often used to regulate gut microbial colonization. This study aims to test whether early intervention with FMT using fecal microbiota from gestation sows combined with Clostridium butyricum and Saccharomyces boulardii (FMT-CS) administration could promote the maturation of gut microbiota and development of immune system in piglets. Piglets were assigned to control (n = 84) and FMT-CS treatment (n = 106), which were treated with placebo and bacterial suspension during the first three days after birth, respectively. By 16S rRNA gene sequencing, we found that FMT-CS increased the α-diversity and reduced the unweighted UniFrac distances of the OTU community. Besides, FMT-CS increased the relative abundance of beneficial bacteria, while decreasing that of opportunistic pathogens. FMT-CS also enhanced the relative abundance of genes related to cofactors and vitamin, energy, and amino acid metabolisms during the early-life period. ELISA analysis revealed that FMT-CS gave rise to the plasma concentrations of IL-23, IL-17, and IL-22, as well as the plasma levels of anti-M.hyo and anti-PCV2 antibodies. Furthermore, the FMT-CS-treated piglets showed decreases in inflammation levels and oxidative stress injury, and improvement of intestinal barrier function after weaning as well. Taken together, our results suggest that early-life intervention with FMT-CS could promote the development of innate and adaptive immune system and vaccine efficacy, and subsequently alleviate weaning stress through promoting the maturation of gut microbiota in piglets.

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Section: Early-life Intervention With Fmt-cs Promotes the Developmentmentioning

confidence: 60%

Section: Early Intervention With Fmt-cs Increases Beneficial Bacteriamentioning

confidence: 70%

Early-Life Intervention Using Fecal Microbiota Combined with Probiotics Promotes Gut Microbiota Maturation, Regulates Immune System Development, and Alleviates Weaning Stress in Piglets

Xiang

Pan

et al. 2020

IJMS

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“…The application of radiomics to PDAC has generated optimism, but it is also challenging because of nonspecific clinical presentation and subtle imaging findings. Previous studies on the application of radiomics to PDAC have focused on prognostic assessments and differential diagnosis [40][41][42][43]. The current study used radiomics features of the entire 3D volume to assess preoperative LN status in patients with PDAC.…”

Section: Discussionmentioning

confidence: 99%

Contrast-enhanced CT radiomics for predicting lymph node metastasis in pancreatic ductal adenocarcinoma: a pilot study

Yao

Xiao

et al. 2020

Cancer Imaging

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Background:We developed a computational model integrating clinical data and imaging features extracted from contrast-enhanced computed tomography (CECT) images, to predict lymph node (LN) metastasis in patients with pancreatic ductal adenocarcinoma (PDAC).Methods: This retrospective study included 159 patients with PDAC (118 in the primary cohort and 41 in the validation cohort) who underwent preoperative contrast-enhanced computed tomography examination between 2012 and 2015. All patients underwent surgery and lymph node status was determined. A total of 2041 radiomics features were extracted from venous phase images in the primary cohort, and optimal features were extracted to construct a radiomics signature. A combined prediction model was built by incorporating the radiomics signature and clinical characteristics selected by using multivariable logistic regression. Clinical prediction models were generated and used to evaluate both cohorts. Results: Fifteen features were selected for constructing the radiomics signature based on the primary cohort. The combined prediction model for identifying preoperative lymph node metastasis reached a better discrimination power than the clinical prediction model, with an area under the curve of 0.944 vs. 0.666 in the primary cohort, and 0.912 vs. 0.713 in the validation cohort.Conclusions: This pilot study demonstrated that a noninvasive radiomics signature extracted from contrastenhanced computed tomography imaging can be conveniently used for preoperative prediction of lymph node metastasis in patients with PDAC.

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“…Antibiotic treatment-induced dysbiosis can have widereaching effects, for example on inflammation, infection and responses to vaccination [70][71][72]. Antibiotics have been shown to affect SCFA-producing bacteria causing a decrease in SCFA abundance [73,74].…”

Section: Effect Of Commensal Metabolites On Host Immune Functionmentioning

confidence: 99%

Metabolism at the centre of the host–microbe relationship

Maslowski

2019

Clinical and Experimental Immunology

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Summary Maintaining homoeostatic host–microbe interactions is vital for host immune function. The gut microbiota shapes the host immune system and the immune system reciprocally shapes and modifies the gut microbiota. However, our understanding of how these microbes are tolerated and how individual, or communities of, gut microbes influence host function is limited. This review will focus on metabolites as key mediators of this complex host–microbe relationship. It will look at the central role of epithelial metabolism in shaping the gut microbiota, how microbial metabolites influence the epithelium and the mucosal and peripheral immune system, and how the immune system shapes microbial composition and metabolism. Finally, this review will look at how metabolites are involved in cross‐talk between different members of the microbiota and their role during infections.

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Early-Life Antibiotic-Driven Dysbiosis Leads to Dysregulated Vaccine Immune Responses in Mice

Cited by 150 publications

References 51 publications

Early-Life Intervention Using Fecal Microbiota Combined with Probiotics Promotes Gut Microbiota Maturation, Regulates Immune System Development, and Alleviates Weaning Stress in Piglets

Early-Life Intervention Using Fecal Microbiota Combined with Probiotics Promotes Gut Microbiota Maturation, Regulates Immune System Development, and Alleviates Weaning Stress in Piglets

Contrast-enhanced CT radiomics for predicting lymph node metastasis in pancreatic ductal adenocarcinoma: a pilot study

Metabolism at the centre of the host–microbe relationship

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