Early leaf senescence is associated with an altered cellular redox balance in Arabidopsis cpr5/old1 mutants

Abstract

Early leaf senescence is associated with an altered cellular redox balance in Arabidopsis cpr5/old1 mutants Jing, H. -C.; Hebeler, R.; Oeljeklaus, S.; Sitek, B.; Stuehler, K.; Meyer, H. E.; Sturre, M. J. G.; Hille, Jacob; Warscheid, B.; Dijkwel, P. P. Link to publication in University of Groningen/UMCG research database Citation for published version (APA): Jing, H. -C., Hebeler, R., Oeljeklaus, S., Sitek, B., Stuehler, K., Meyer, H. E., ... Stühler, K. (2008). Early leaf senescence is associated with an altered cellular redox balance in Arabidopsis cpr5/old1 mutants. Plant Biology, 10(1), 85-98. DOI: 10.111185-98. DOI: 10. /j.143885-98. DOI: 10. -8677.2008 Copyright Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum. INTRODUCTIONAerobic life depends on oxygen. However, oxygen atoms and oxygen-containing molecules can act as highly reactive molecules, commonly known as reactive oxygen species (ROS), when unpaired electrons are present. ROS include triplet and singlet oxygen, superoxide, nitric oxide and hydroxyl radicals, which can potentially damage and deteriorate various cellular components, such as proteins, DNA and lipids, by oxidation. Thus, oxygen is inherently detrimental to aerobic organisms. The dual effect of oxygen on shaping life-history traits and evolution of aerobic organisms is generally known as the 'Oxygen Paradox' (Davies 1995). ROS can be generated by internal organelles and other cellular components, including mitochondria, chloroplasts and microbodies, as well as by external stress conditions, such as ozone exposure and UV-B radiation. To cope with the danger imposed by ROS, cells have evolved sophisticated ROS scavenging systems. These include antioxidative enzymes such as catalase, superoxide dismutase and ascorbate peroxidase, as well as non-enzymatic antioxidants such as ascorbate, the tripeptide glutathione, tocopherol and carotenoids (Apel & Hirt 2004). In addition to being a 'wear-and-tear' force, ROS are important cellular signalling molecules in the cell cycle, hormonal signalling, programmed cell death, growth and development and responses to biotic and abiotc stresses (Finkel 2003;Foyer & Noctor 2005; Keywords Cell death; CPR5 ⁄ OLD1; leaf senescence; reactive oxygen species; redox balance. ABSTRACTReactive oxygen species (ROS) are the inevitable by-products of essential cellular metabolic and physiological activities. Plants have developed sophisticated gene networks of ROS generation and sc...

Metabolic Regulation of Leaf Senescence in Sunflower (Helianthus annuus L.) Plants

“…A simultaneous increase in lipid peroxidation was observed. Mutations in the Arabidopsis CPR5/OLD1 gene may cause early senescence through deregulation of the cellular redox balance (Jing et al, 2008). Also, there is evidence suggesting that inadequate oxidant and carbonyl group production are intrinsically related to plant ageing, and that low mitochondrial, superoxide dismutase and ascorbate peroxidase activities may contribute to extensive protein carbonylation (Vanacker et al, 2006;Srivalli & Khanna-Chopra, 2009).…”

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“…This simplistic, although often used, comparative effort provides a straightforward way to determine a common set of transcriptomic changes and is a good starting point for an initial comparison/assessment of the data sets (Larsson et al, 2006). Our pioneering meta-analysis turned out to be a useful framework for subsequent studies that assessed the involvement of ROS signaling in various biological processes in plants (Jing et al, 2008;Rosenwasser et al, 2011;Peng et al, 2014;Jozefczak et al, 2015).…”

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“…9 This is consistent to our results showing that blocking a particular signalling pathway did not affect the alterations in other signalling pathways in cpr5 mutants. 11 For instance, cpr5-induced ethylene hypersensitivity was converted into ethylene insensitivity in cpr5ein2 double mutants, but cpr5ein2 seedlings still exhibited hypersensitivity to sugar, ABA and jasmonic acid. Similarly, cpr5abi4 double mutants were sugar insensitive but were still hypersensitive to ethylene and jasmonic acid.…”

“…We therefore examined the transcriptomic and proteomic profiles of pre-symptom cpr5 mutants. 10,11 Using 5-fold increase as a cut-off threshold three of the five universal ROS marker genes, 16 of the 27 genes induced by six ROS treatments and one third of the ROS-dependent putative transcription factors were upregulated in cpr5 mutants. Proteins clearly exhibiting increased abundance were predominantly members of the detoxifying enzyme family of glutathione S-transferases (GSTs).…”