Early Interaction of Alternaria infectoria Conidia with Macrophages

Almeida, Mariana Cruz; Antunes, Daniela; Silva, B M A; Rodrigues, Lisa; Mota, Marta; Borges, Olga; Fernandes, Chantal; Gonçalves, Teresa

doi:10.1007/s11046-019-00339-6

Cited by 6 publications

(4 citation statements)

References 41 publications

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“…Macrophage-yeast interaction assays were carried out as previously described [30]. Briefly, RAW 264.7 macrophage cells were platted in 96-, in 12-well (with 16 mm glass coverslips) or in µ-slide 8 well plates, and incubated for 18 h at 37°C, under a 5% CO2 atmosphere.…”

Section: Macrophage-yeast Interaction Assaysmentioning

confidence: 99%

The transcription factor Ndt80 is a repressor of Candida parapsilosis virulence attributes

et al. 2021

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Candida parapsilosis is an emergent opportunistic yeast among hospital settings that affects mainly neonates and immunocompromised patients. Its most remarkable virulence traits are the ability to adhere to prosthetic materials, as well as the formation of biofilm on abiotic surfaces. The Ndt80 transcription factor was identified as one of the regulators of biofilm formation by C. parapsilosis; however, its function in this process was not yet clarified. By knocking out NDT80 (CPAR2-213640) gene, or even just one single copy of the gene, we observed substantial alterations of virulence attributes, including morphogenetic changes, adhesion and biofilm growth profiles. Both ndt80Δ and ndt80ΔΔ mutants changed colony and cell morphologies from smooth, yeast-shaped to crepe and pseudohyphal elongated forms, exhibiting promoted adherence to polystyrene microspheres and notably, forming a higher amount of biofilm compared to wild-type strain. Interestingly, we identified transcription factors Ume6, Cph2, Cwh41, Ace2, Bcr1, protein kinase Mkc1 and adhesin Als7 to be under Ndt80 negative regulation, partially explaining the phenotypes displayed by the ndt80ΔΔ mutant. Furthermore, ndt80ΔΔ pseudohyphae adhered more rapidly and were more resistant to murine macrophage attack, becoming deleterious to such cells after phagocytosis. Unexpectedly, our findings provide the first evidence for a direct role of Ndt80 as a repressor of C. parapsilosis virulence attributes. This finding shows that C. parapsilosis Ndt80 functionally diverges from its homolog in the close related fungal pathogen C. albicans.

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Section: Macrophage-yeast Interaction Assaysmentioning

confidence: 99%

The transcription factor Ndt80 is a repressor of Candida parapsilosis virulence attributes

et al. 2021

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“…Moreover, the human pathogenic fungi could influence various important systems and functions of human. For example, A. infectoria was harmful for the human skin and immune function ( Almeida et al, 2019 , Kieselová et al, 2021 ). C. sake , a commonly pathogenic fungus, had a close relationship with the human immunodeficiency virus (HIV) ( Hoegl, Schönian, Ollert, & Korting, 1998 ).…”

Section: Discussionmentioning

confidence: 99%

DNA metabarcoding analysis of fungal community on surface of four root herbs

Dao

Yang

et al. 2024

Chinese Herbal Medicines

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“…RPMI 1640 medium (Sigma-Aldrich) supplemented with 10% inactivated FBS, 23.8 mM sodium bicarbonate and 50 mM glucose was used for the experimental assays. For the co-culture assays with RAW 264.7 cells, a previously optimized procedure was performed [ 14 , 15 ]. Briefly, 1 × 10 5 cells per well were seeded in a 96-well plate and incubated for 18 h. Thereafter, the culture medium was removed and cells were washed twice with sterile pre-warmed PBS 1X.…”

Section: Methodsmentioning

confidence: 99%

Vulvovaginal Candida albicans Clinical Isolates’ Resistance to Phagocytosis In-Vitro

Faria-Gonçalves

Oliveira

Gaspar

et al. 2022

Life

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Previous studies have revealed that Candida albicans isolates involved in chronic vulvovaginal candidosis (cVVC) phenotypically express less virulent traits than clinical isolates involved in sporadic infections. In this study, we aimed to further explore this finding by studying the behaviour of those same clinical isolates in in-vitro models of infection. Eighteen clinical Candida albicans isolates were collected from women suffering sporadic (eight isolates) or chronic infections (ten isolates). Adhesion to HeLa cells (human cervical cancer epithelial cell line) and resistance to phagocytosis by RAW 264.7 cells (murine macrophages cell line) were tested in-vitro. In addition, phenotypic expression of virulence factors related with either adhesion or resistance to phagocytosis was tested in-vitro. Results indicated that yeast isolates involved in sporadic infection adhered in a higher proportion of HeLa cells than those of chronic infections, which was related with their ability to produce biofilm (p < 0.05). The ability to evade phagocytosis was related to an elevated production of proteases (p < 0.05) by chronic isolates, while sporadic isolates’ resistance to phagocytosis was related to a higher hydrophobicity of cell walls (p < 0.05). We conclude that the evasion of macrophage-mediated phagocytosis related to the production of proteases might be an important factor involved in the recurrence of vulvovaginal candidosis infection.

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Early Interaction of Alternaria infectoria Conidia with Macrophages

Cited by 6 publications

References 41 publications

The transcription factor Ndt80 is a repressor of Candida parapsilosis virulence attributes

The transcription factor Ndt80 is a repressor of Candida parapsilosis virulence attributes

DNA metabarcoding analysis of fungal community on surface of four root herbs

Vulvovaginal Candida albicans Clinical Isolates’ Resistance to Phagocytosis In-Vitro

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