2006
DOI: 10.1111/j.1365-313x.2006.02836.x
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Early infection of scutellum tissue with Agrobacterium allows high‐speed transformation of rice

Abstract: SummarySeveral approaches have recently been adopted to improve Agrobacterium-mediated transformation of rice, both to generate the large number of T-DNA insertion plants needed for functional analysis of the rice genome, and for production of rice with additional agronomical value. However, about 3 months of in vitro culture is still required for isolation of transgenic rice plants. Here, we report the competency of scutellum tissue from 1-day pre-cultured seeds for Agrobacterium-mediated transformation. Furt… Show more

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Cited by 629 publications
(485 citation statements)
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References 31 publications
(30 reference statements)
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“…Rice stable transformation via Agrobacterium tumefaciens has become a routine technique in laboratories (Toki et al 2006). Nowadays, even if japonica is frequently used because it is highly amenable to transformation, indica transformation efficiency has sufficiently improved to allow its use in functional validation experiments and field trials in the appropriate environments.…”
Section: Introductionmentioning
confidence: 99%
“…Rice stable transformation via Agrobacterium tumefaciens has become a routine technique in laboratories (Toki et al 2006). Nowadays, even if japonica is frequently used because it is highly amenable to transformation, indica transformation efficiency has sufficiently improved to allow its use in functional validation experiments and field trials in the appropriate environments.…”
Section: Introductionmentioning
confidence: 99%
“…1a) used in this study (Toki 1997;Toki et al 2006) was transferred into Agrobacterium tumefaciens strain EHA105 by electroporation (Hood et al 1993). …”
Section: Binary Vector and Agrobacterium Transformationmentioning
confidence: 99%
“…Selection against hygromycin (Hyg) and regeneration of transgenic plants were performed following the method of Toki et al (2006). Visual selection of GFP was performed as follows: after 3-days of co-cultivation with Agrobacterium at 25°C under constant dark, the calli were washed and cultured on N6D medium containing 400 mg/L carbenicillin (Nakalai tesque, Kyoto, Japan) for 4-8 weeks at 33°C (light for 10 h)/30°C (dark for 14 h).…”
Section: Visual Selection Proceduresmentioning
confidence: 99%
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