EARLY FLOWERING4 Recruitment of EARLY FLOWERING3 in the Nucleus Sustains the <i>Arabidopsis</i> Circadian Clock

Herrero, Eva; Kolmos, Elsebeth; Bujdoso, Nora; Yuan, Ye; Meng-Meng, Wang; Berns, Markus Christian; Uhlworm, Heike; Coupland, George; Saini, Reena V.; Jaskólski, Mariusz; Webb, Alex A. R.; Gonçalves, Jorge; Davis, Seth J

doi:10.1105/tpc.111.093807

Cited by 271 publications

(339 citation statements)

References 68 publications

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“…In diurnal conditions, PIF4 and PIF5 transcript levels start to rise at midday through the night, with a peak at dawn (Nozue et al, 2007). The evening complex (EC) formed by ELF3, ELF4, and LUX is necessary for this expression pattern Herrero et al, 2012;Lu et al, 2012). LUX has been found to directly bind to PIF4/5 promoters through the LUX binding site (GATA/TCG) and recruit ELF3 and ELF4.…”

Section: Transcriptional Regulation Of the Pifsmentioning

confidence: 99%

PIFs: Systems Integrators in Plant Development

2014

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Section: Transcriptional Regulation Of the Pifsmentioning

confidence: 99%

PIFs: Systems Integrators in Plant Development

2014

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“…2G,3A,and 6,A and B). ELF4 forms part of the Evening Complex (Nusinow et al, 2011), which directly represses expression of clock genes such as PRR7, PRR9, GI, and ARRHYTHMO (LUX; Herrero et al, 2012;Mizuno et al, 2014;Box et al, 2015). Intriguingly, ELF4 also is necessary for red light-mediated induction of CCA1 and LHY (Kikis et al, 2005).…”

Section: Yhb Mimics Continuous Light Input Into the Circadian Systemmentioning

confidence: 99%

A Constitutively Active Allele of Phytochrome B Maintains Circadian Robustness in the Absence of Light

Jones

Litthauer

et al. 2015

Plant Physiology

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The sensitivity of the circadian system to light allows entrainment of the clock, permitting coordination of plant metabolic function and flowering time across seasons. Light affects the circadian system via both photoreceptors, such as phytochromes and cryptochromes, and sugar production by photosynthesis. In the present study, we introduce a constitutively active version of phytochrome B-Y276H (YHB) into both wild-type and phytochrome null backgrounds of Arabidopsis (Arabidopsis thaliana) to distinguish the effects of photoreceptor signaling on clock function from those of photosynthesis. We find that the YHB mutation is sufficient to phenocopy red light input into the circadian mechanism and to sustain robust rhythms in steady-state mRNA levels even in plants grown without light or exogenous sugars. The pace of the clock is insensitive to light intensity in YHB plants, indicating that light input to the clock is constitutively activated by this allele. Mutation of YHB so that it is retained in the cytoplasm abrogates its effects on clock function, indicating that nuclear localization of phytochrome is necessary for its clock regulatory activity. We also demonstrate a role for phytochrome C as part of the red light sensing network that modulates phytochrome B signaling input into the circadian system. Our findings indicate that phytochrome signaling in the nucleus plays a critical role in sustaining robust clock function under red light, even in the absence of photosynthesis or exogenous sources of energy.

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“…The proteins encoded by these genes form a complex termed the evening complex (EC), in which the ELF3 protein is suggested to act as a molecular scaffold and signaling hub, connecting ELF4 with LUX ). ELF4 appears to have a role in determining the subcellular location of the complex (Herrero et al, 2012) and, thus, possibly in directing the activity of the LUX transcription factor. Loss of any one of these proteins compromises the EC function , an interaction that is likely to explain the generally similar phenotypes of elf3, elf4, and lux mutants.…”

mentioning

confidence: 99%

EARLY FLOWERING3 Redundancy Fine-Tunes Photoperiod Sensitivity

et al. 2017

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Three pea (Pisum sativum) loci controlling photoperiod sensitivity, HIGH RESPONSE (HR), DIE NEUTRALIS (DNE), and STERILE NODES (SN), have recently been shown to correspond to orthologs of Arabidopsis (Arabidopsis thaliana) circadian clock genes EARLY FLOWERING3 (ELF3), ELF4, and LUX ARRHYTHMO, respectively. A fourth pea locus, PHOTOPERIOD (PPD), also contributes to the photoperiod response in a similar manner to SN and DNE, and recessive ppd mutants on a springflowering hr mutant background show early, photoperiod-insensitive flowering. However, the molecular identity of PPD has so far remained elusive. Here, we show that the PPD locus also has a role in maintenance of diurnal and circadian gene expression rhythms and identify PPD as an ELF3 co-ortholog, termed ELF3b. Genetic interactions between pea ELF3 genes suggest that loss of PPD function does not affect flowering time in the presence of functional HR, whereas PPD can compensate only partially for the lack of HR. These results provide an illustration of how gene duplication and divergence can generate potential for the emergence of more subtle variations in phenotype that may be adaptively significant.

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EARLY FLOWERING4 Recruitment of EARLY FLOWERING3 in the Nucleus Sustains the Arabidopsis Circadian Clock

Cited by 271 publications

References 68 publications

PIFs: Systems Integrators in Plant Development

PIFs: Systems Integrators in Plant Development

A Constitutively Active Allele of Phytochrome B Maintains Circadian Robustness in the Absence of Light

EARLY FLOWERING3 Redundancy Fine-Tunes Photoperiod Sensitivity

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