Early flowering and increased expression of a FLOWERING LOCUS T-like gene in chrysanthemum transformed with a mutated ethylene receptor gene mDG-ERS1(etr1-4)

Morita, Shigeto; Murakoshi, Yuino; Hojo, Ai; Chisaka, Keiko; Harada, Taro; Satoh, Shigeru

doi:10.1007/s12374-012-0109-8

Cited by 3 publications

(1 citation statement)

References 23 publications

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“…However, other phytohormones except for GA are also thought to influence flowering time. For example, ethylene, the first plant hormone discovered, has been shown to delay flowering time in both Arabidopsis and chrysanthemum (Achard et al., 2007; Morita et al., 2012), although further research is required to elucidate its mechanism in flowering.…”

Section: Introductionmentioning

confidence: 99%

The RAV transcription factor TEMPRANILLO1 involved in ethylene‐mediated delay of chrysanthemum flowering

Cheng,

Wang,

Zhang

et al. 2023

The Plant Journal

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SUMMARYTEMPRANILLO1 (TEM1) is a transcription factor belonging to related to ABI3 and VP1 family, which is also known as ethylene response DNA‐binding factor 1 and functions as a repressor of flowering in Arabidopsis. Here, a putative homolog of AtTEM1 was isolated and characterized from chrysanthemum, designated as CmTEM1. Exogenous application of ethephon leads to an upregulation in the expression of CmTEM1. Knockdown of CmTEM1 promotes floral initiation, while overexpression of CmTEM1 retards floral transition. Further phenotypic observations suggested that CmTEM1 involves in the ethylene‐mediated inhibition of flowering. Transcriptomic analysis established that expression of the flowering integrator CmAFL1, a member of the APETALA1/FRUITFULL subfamily, was downregulated significantly in CmTEM1‐overexpressing transgenic plants compared with wild‐type plants but was verified to be upregulated in amiR‐CmTEM1 lines by quantitative RT‐PCR. In addition, CmTEM1 is capable of binding to the promoter of the CmAFL1 gene to inhibit its transcription. Moreover, the genetic evidence supported the notion that CmTEM1 partially inhibits floral transition by targeting CmAFL1. In conclusion, these findings demonstrate that CmTEM1 acts as a regulator of ethylene‐mediated delayed flowering in chrysanthemum, partly through its interaction with CmAFL1.

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Section: Introductionmentioning

confidence: 99%

The RAV transcription factor TEMPRANILLO1 involved in ethylene‐mediated delay of chrysanthemum flowering

Cheng,

Wang,

Zhang

et al. 2023

The Plant Journal

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Isolation and characterization of differentially expressed genes in petals of chrysanthemum mutant cultivars developed by irradiation

Kim

Sung

Kim

et al. 2015

Scientia Horticulturae

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Transcriptome Analysis of Ethylene Response in Chrysanthemum moriflolium Ramat. with an Emphasis on Flowering Delay

Cheng

Zhou

et al. 2023

Horticulturae

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Ethylene is a gaseous phytohormone that delays flowering in Chrysanthemum morifolium Ramat. To date, however, there have been no systematic studies on genes involved in the ethylene response of this species, and the mechanism underlying ethylene-delayed flowering remains unclear. Herein, we applied RNA sequencing to characterize the ethylene response by comparing the transcriptomes of chrysanthemum cultivar ‘Jinba’ with or without ethephon treatment. Six unique RNA-seq libraries were generated. The identified differentially expressed genes (DEGs) primarily involved ethylene, auxin, and abscisic acid signaling genes; circadian clock genes; genes encoding functional proteins associated with floral transition, such as homologs of AP1/FRUITFUL-like 1 (AFL1), TERMINAL FLOWER 1 (TFL1), and so on; and genes encoding transcription factors, specifically of the MYB and bHLH families. Furthermore, quantitative RT-PCR was used to verify the DEGs identified by RNA-seq. Heterologous CmAFL1 overexpression in Arabidopsis thaliana resulted in early flowering. Our findings present a landscape of transcriptomes and reveal the candidate genes involved in the ethylene-mediated regulation of flowering time in chrysanthemum, providing useful data for further studies.

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Early flowering and increased expression of a FLOWERING LOCUS T-like gene in chrysanthemum transformed with a mutated ethylene receptor gene mDG-ERS1(etr1-4)

Cited by 3 publications

References 23 publications

The RAV transcription factor TEMPRANILLO1 involved in ethylene‐mediated delay of chrysanthemum flowering

The RAV transcription factor TEMPRANILLO1 involved in ethylene‐mediated delay of chrysanthemum flowering

Isolation and characterization of differentially expressed genes in petals of chrysanthemum mutant cultivars developed by irradiation

Transcriptome Analysis of Ethylene Response in Chrysanthemum moriflolium Ramat. with an Emphasis on Flowering Delay

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