Early Evidence for Zika Virus Circulation among <i>Aedes aegypti</i> Mosquitoes, Rio de Janeiro, Brazil

Ayllón, Tania; Campos, Renata de Mendonça; Brasil, Patrícia; Morone, Fernanda; Câmara, Daniel Cardoso Portela; Meira, Guilherme Louzada Silva; Tannich, Egbert; Yamamoto, Kristie Aimi; Carvalho, Marília Sá; Pedro, Renata Saraiva; Schmidt‐Chanasit, Jonas; Cadar, Dániel; Ferreira, Davis Fernandes; Honório, Nildimar Alves

doi:10.3201/eid2308.162007

Cited by 43 publications

(50 citation statements)

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“…Surveillance of ZIKV in mosquitoes is an important measure for identifying potential entry points and monitoring virus activity [93]. LAMP has been evaluated for the detection of the ZIKV in mosquito samples by many groups, including ourselves [27,41,42,53].…”

Section: Lamp For Detection Of Zikv In Mosquito Samplesmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review

Silva

Pardee

Pena

2019

Viruses

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The recent outbreak of Zika virus (ZIKV) in the Americas and its devastating developmental and neurological manifestations has prompted the development of field-based diagnostics that are rapid, reliable, handheld, specific, sensitive, and inexpensive. The gold standard molecular method for lab-based diagnosis of ZIKV, from either patient samples or insect vectors, is reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The method, however, is costly and requires lab-based equipment and expertise, which severely limits its use as a point-of-care (POC) tool in resource-poor settings. Moreover, given the lack of antivirals or approved vaccines for ZIKV infection, a POC diagnostic test is urgently needed for the early detection of new outbreaks and to adequately manage patients. Loop-mediated isothermal amplification (LAMP) is a compelling alternative to RT-qPCR for ZIKV and other arboviruses. This low-cost molecular system can be freeze-dried for distribution and exhibits high specificity, sensitivity, and efficiency. A growing body of evidence suggests that LAMP assays can provide greater accessibility to much-needed diagnostics for ZIKV infections, especially in developing countries where the ZIKV is now endemic. This review summarizes the different LAMP methods that have been developed for the virus and summarizes their features, advantages, and limitations.Viruses 2020, 12, 19 2 of 20 raising concerns about the neurological tropism of the virus [12]. In May 2015, the first case of ZIKV infection was reported in Brazil [13] and the virus rapidly spread throughout the country and much of Latin America, causing the largest recorded epidemic of the virus to date [14]. The Brazilian epidemic raised great international concern because of severe birth defects, including microcephaly, in neonates born to mothers infected by ZIKV during pregnancy [3,15,16].ZIKV is transmitted mainly through the bite of infected mosquitoes from the genus Aedes, although other vectors may also be involved in the transmission [17][18][19][20][21]. Additionally, other routes of ZIKV transmission have been identified, including blood transfusions, transplacental, perinatal, and sexual intercourse [22][23][24]. ZIKV infection usually causes a self-limiting and a mild illness, where the majority of cases are asymptomatic and, when present, symptoms include fever, headache, rash, conjunctivitis, and arthralgia [25]. In regions where there is a circulation of other arboviruses, such as DENV and chikungunya (CHIKV), the clinical diagnosis of ZIKV infection becomes extremely difficult because of common symptoms. Therefore, laboratory-based molecular diagnosis is of fundamental importance to correctly identify the etiologic agent [3,26].Given the lack of approved vaccines and antivirals against ZIKV, a rapid and reliable point-of-care (POC) diagnostic test for detection of ZIKV is urgently required for control and prevention measures and to increase the diagnostic capacity of ZIKV-affected, mainly in low-resource areas [27,28]. Z...

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Section: Lamp For Detection Of Zikv In Mosquito Samplesmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review

Silva

Pardee

Pena

2019

Viruses

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“…There is extensive evidence that Ae. aegypti mosquitoes are able to transmit Zika virus in both the laboratory [117–128] and in the field [129,130]. Ae.…”

Section: Resultsmentioning

confidence: 99%

Arbovirus Vectors of Epidemiological Concern in the Americas: A Scoping Review of Entomological Studies on Zika, Dengue and Chikungunya Virus Vectors

Jones

Kulkarni

Davidson

et al. 2019

Preprint

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27 Background 28 Three arthorpod-borne viruses (arboviruses) causing human disease have been the focus 29 of a large number of studies in the Americas since 2013 due to their global spread and 30 epidemiological impacts: Zika, dengue, and chikungunya viruses. A large proportion of 31 infections by these viruses are asymptomatic. However, all three viruses are associated with 32 moderate to severe health consequences in a small proportion of cases. Two mosquito species, 33 Aedes aegypti and Aedes albopictus, are among the world's most prominent arboviral vectors, 34 and are known primary vectors for all three viruses in the Americas. 35 Objectives 2 36 This review summarizes the state of the entomological literature surrounding the biology 37 and ecology of vectors of Zika, dengue and chikungunya viruses and factors affecting virus 38 transmission. The rationale of the review was to elucidate consensus and discord between 39 studies, and guide future research based on identified knowledge gaps. 40 Results 41 A total of 196 studies were included in the scoping review after initial screening and 42 subsequent exclusion of out-of-scope studies, secondary data publications, duplicate records, and 43 studies unavailable in English language. 44 Key findings 45 Temperature and humidity have the strongest impact on mosquito distribution and 46 dynamics, development of immatures and arborviral infection rates. Low socioeconomic status 47 and related factors, including poor infrastructure, inconsistent access to water, and high 48 household resident density, have been consistently associated with arbovirus vector occurrence. 49 Effects of interspecific competition on arboviral vector species is currently poorly understood. 50 Vector competence for Zika virus is well established for Ae. aegypti and Ae. albopictus. 51 Information on Zika virus vector transmission dynamics is sparse in contrast to the wealth of 52 research available for dengue and chikungunya viruses. 53 Conclusions 54Based on the internationally recognized urgency of Zika virus infection as a public health 55 concern, further research on arbovirus vectors and transmission dynamics is of pressing need. 56 57

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“…The geographical presence of this mosquito vector has dramatically increased in the last few decades, leading to an expanded transmission of these arboviruses (12–14). The lack of vaccines and treatment against these arboviruses highlights the importance of mosquito control and surveillance strategies (15). Current and future mosquito-targeted control strategies are, and will be, having a significant epidemiological impact but also require mosquito and pathogen surveillance (15).…”

Section: Introductionmentioning

confidence: 99%

“…The lack of vaccines and treatment against these arboviruses highlights the importance of mosquito control and surveillance strategies (15). Current and future mosquito-targeted control strategies are, and will be, having a significant epidemiological impact but also require mosquito and pathogen surveillance (15). Surveillance of geographical distribution of the vector mosquitoes and the pathogens they carry is an essential component of disease prevention and control.…”

Section: Introductionmentioning

confidence: 99%

Field-deployable molecular diagnostic platform for arbovirus and Wolbachia detection in Aedes aegypti

Rutkowski

Dong

Dimopoulos

2020

Preprint

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26Background: Surveillance of mosquito infection status is critical for planning and deployment 27 of proper mosquito control initiatives. Concurrently, Wolbachia is being widely used as a control 28 method for arboviral transmission. Point-of-care (POC) detection assays are necessary for 29 monitoring the infection prevalence and geographic range of viruses as well as Wolbachia in 30 mosquito vector populations. We therefore assessed the novel qPCR bCUBE molecular 31 diagnostic system as a tool for virus and Wolbachia detection. 32 Results: We developed a reliable, specific, and sensitive diagnostic assay for detecting Zika 33 virus and dengue virus serotype 2 using the real-time qPCR platform bCUBE. With bCUBE-34 based qRT-PCR, both Wolbachia bacterium and virus RNA could be reliably detected in 35 individually infected Ae. aegypti mosquitoes and in pools of 5, 10, or 15 mosquitoes. 36 Conclusions: The portable qPCR bCUBE diagnostic platform is capable of detecting Zika and 37 dengue virus as well as Wolbachia in mosquitoes and therefore has potential as a practical field-38 deployable diagnostic test for vector-borne disease surveillance programs. 39 40 41 42 43 44 45 46 47

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Early Evidence for Zika Virus Circulation among Aedes aegypti Mosquitoes, Rio de Janeiro, Brazil

Cited by 43 publications

References 10 publications

Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review

Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review

Arbovirus Vectors of Epidemiological Concern in the Americas: A Scoping Review of Entomological Studies on Zika, Dengue and Chikungunya Virus Vectors

Field-deployable molecular diagnostic platform for arbovirus and Wolbachia detection in Aedes aegypti

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