Early Events in the Ethylene Biosynthetic Pathway – Regulation of the Pools of Methionine and<i>S</i>‐Adenosylmethionine

Bürstenbinder, Katharina; Sauter, Margret

doi:10.1002/9781118223086.ch2

Cited by 13 publications

(9 citation statements)

References 148 publications

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“…Of interest is the antioxidant GSH, which has been a central molecule determi-nant of cellular redox homeostasis (Noctor et al, 2012). Moreover, the biosynthesis of methionine from cysteine resulted in the hormone ethylene, polyamines, and nicotine amine, all of which are involved to varying degrees in the modulation of the plant response to stresses (Burstenbinder and Sauter, 2012;Liu JH et al, 2015;Lee et al, 2017).…”

Section: Kegg Pathways Of Degsmentioning

confidence: 99%

Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean

et al. 2020

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Genes related to the response to drought stress in leaf and root tissue of drought-susceptible (DS) and tolerant (DT) genotypes were characterized by RNA-Seq. In total, 54,750 transcripts, representative of 28,590 genes, were identified; of these, 1,648 were of high-fidelity (merge of 12 libraries) and described for the first time in the Andean germplasm. From the 1,239 differentially expressed genes (DEGs), 458 were identified in DT, with a predominance of genes in categories of oxidative stress, response to stimulus and kinase activity. Most genes related to oxidation-reduction terms in roots were early triggered in DT (T75) compared to DS (T150) suggestive of a mechanism of tolerance by reducing the damage from ROS. Among the KEGG enriched by DEGs up-regulated in DT leaves, two related to the formation of Sulfur-containing compounds, which are known for their involvement in tolerance to abiotic stresses, were common to all treatments. Through qPCR, 88.64% of the DEGs were validated. A total of 151,283 variants were identified and functional effects estimated for 85,780. The raw data files were submitted to the NCBI database. A transcriptome map revealed new genes and isoforms under drought. These results supports a better understanding of the drought tolerance mechanisms in beans.

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Section: Kegg Pathways Of Degsmentioning

confidence: 99%

Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean

et al. 2020

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“…1) requires decarboxylated S-adenosylmethionine (dcSAM). SAM is a molecule central to ethylene biosynthesis, because it is synthesized from methionine (Met), and acts as substrate for formation of 1-aminocyclopropane-1-carboxylic acid (ACC)-a direct precursor of ethylene (Wang and others 2002;B} urnsterbinder and Sauter 2012). Therefore, a competition between PAs and ethylene biosynthesis is suggested.…”

Section: Introductionmentioning

confidence: 99%

Polyamines and Nitric Oxide Link in Regulation of Dormancy Removal and Germination of Apple (Malus domestica Borkh.) Embryos

Krasuska

Ciąćka

Bogatek

et al. 2013

J Plant Growth Regul

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Polyamines (PAs) belong to plant growth regulators and in complex with classical phytohormones take part in regulation of seed dormancy and germination. Although the impact of reactive oxygen (ROS) and nitrogen (RNS) species on seed germination is well described, the cross talk of PAs with ROS/RNS has never been analyzed. Due to the close connection of PAs and ethylene biosynthetic pathways to arginine (Arg)-dependent NO biosynthesis we investigated production of nitric oxide (NO), peroxynitrite (ONOO -) and the level of O 2 •-or H 2 O 2 in apple embryos, germination of which was PA regulated. PAs: putrescine (Put) and spermidine (Spd) in contrast to spermine (Spm) stimulated germination of apple embryos. Among amino acids, stimulation of germination was observed in Arg and ornithine (Orn) only. Dormancy removal of embryos by PAs was associated with increased accumulation of H 2 O 2 and O 2•-in embryonic axes. At the same stage of completion of sensu stricto germination the stimulatory effect of PAs (Put and Spd) and amino acids, mainly Arg and Orn, was accompanied by enhanced NO and ONOO -production in embryonic axis. The beneficial effect of PAs (Put and Spd) and their precursors on germination of apple embryos was removed by NO scavenging, suggesting a crucial role of NO in termination of embryo germination and radicle growth. Moreover, activity of polyamine oxidase in embryo axes was greatly enhanced by embryo fumigation with NO. Our data demonstrate the interplay of RNS/ROS with PAs and point to NO action as an integrator of endogenous signals activating germination.

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“…Therefore, slightly decreased putrescine and spermidine and significantly decreased SAM contents indicate that they may have been used for spermine synthesis or that spermine may not have been efficiently catalysed. Ethylene is synthesised from SAM [49] and the reduced SAM content might have affected ethylene synthesis. We observed that NbClpC1/C2-suppressed plants completed their life cycle four weeks later than the control plants.…”

Section: Discussionmentioning

confidence: 99%

Co-Suppression of NbClpC1 and NbClpC2, Encoding Clp Protease Chaperons, Elicits Significant Changes in the Metabolic Profile of Nicotiana benthamiana

Ali

Baek

2020

Plants

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Metabolites in plants are the products of cellular metabolic processes, and their differential amount can be regarded as the final responses of plants to genetic, epigenetic, or environmental stresses. The Clp protease complex, composed of the chaperonic parts and degradation proteases, is the major degradation system for proteins in plastids. ClpC1 and ClpC2 are the two chaperonic proteins for the Clp protease complex and share more than 90% nucleotide and amino acid sequence similarities. In this study, we employed virus-induced gene silencing to simultaneously suppress the expression of ClpC1 and ClpC2 in Nicotiana benthamiana (NbClpC1/C2). The co-suppression of NbClpC1/C2 in N. benthamiana resulted in aberrant development, with severely chlorotic leaves and stunted growth. A comparison of the control and NbClpC1/C2 co-suppressed N. benthamiana metabolomes revealed a total of 152 metabolites identified by capillary electrophoresis time-of-flight mass spectrometry. The co-suppression of NbClpC1/C2 significantly altered the levels of metabolites in glycolysis, the tricarboxylic acid cycle, the pentose phosphate pathway, and the purine biosynthetic pathway, as well as polyamine and antioxidant metabolites. Our results show that the simultaneous suppression of ClpC1 and ClpC2 leads to aberrant morphological changes in chloroplasts and that these changes are related to changes in the contents of major metabolites acting in cellular metabolism and biosynthetic pathways.

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Early Events in the Ethylene Biosynthetic Pathway – Regulation of the Pools of Methionine andS‐Adenosylmethionine

Cited by 13 publications

References 148 publications

Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean

Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean

Polyamines and Nitric Oxide Link in Regulation of Dormancy Removal and Germination of Apple (Malus domestica Borkh.) Embryos

Co-Suppression of NbClpC1 and NbClpC2, Encoding Clp Protease Chaperons, Elicits Significant Changes in the Metabolic Profile of Nicotiana benthamiana

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