Early environmental enrichment affects neurobehavioral development and prevents brain damage in rats submitted to neonatal hypoxia-ischemia

Schuch, Clarissa Pedrini; Díaz, Ramiro Guedes; Deckmann, Iohanna; Rojas, Joseane Jiménez; Deniz, Bruna Ferrary; Pereira, Lenir Orlandi

doi:10.1016/j.neulet.2016.02.015

Cited by 34 publications

(24 citation statements)

References 42 publications

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“…Ear unfurling, fur appearance, teeth eruption, and eye opening all mark significant milestones in offspring development (Heyser, 2004), and impaired development of these milestones may predict later behavioral changes (Schuch et al, 2016). Eye opening was the only milestone significantly delayed in the current study.…”

Section: Discussionmentioning

confidence: 99%

Dietary choline levels modify the effects of prenatal alcohol exposure in rats

Idrus

Breit

Thomas

2017

Neurotoxicology and Teratology

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Prenatal alcohol exposure can cause a range of physical and behavioral alterations; however, the outcome among children exposed to alcohol during pregnancy varies widely. Some of this variation may be due to nutritional factors. Indeed, higher rates of fetal alcohol spectrum disorders (FASD) are observed in countries where malnutrition is prevalent. Epidemiological studies have shown that many pregnant women throughout the world may not be consuming adequate levels of choline, an essential nutrient critical for brain development, and a methyl donor. In this study, we examined the influence of dietary choline deficiency on the severity of fetal alcohol effects. Pregnant Sprague-Dawley rats were randomly assigned to receive diets containing 40, 70, or 100% recommended choline levels. A group from each diet condition was exposed to ethanol (6.0 g/kg/day) from gestational day 5 to 20 via intubation. Pair-fed and ad lib lab chow control groups were also included. Physical and behavioral development was measured in the offspring. Prenatal alcohol exposure delayed motor development, and 40% choline altered performance on the cliff avoidance task, independent of one another. However, the combination of low choline and prenatal alcohol produced the most severe impairments in development. Subjects exposed to ethanol and fed the 40% choline diet exhibited delayed eye openings, significantly fewer successes in hind limb coordination, and were significantly overactive compared to all other groups. These data suggest that suboptimal intake of a single nutrient can exacerbate some of ethanol’s teratogenic effects, a finding with important implications for the prevention of FASD.

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Section: Discussionmentioning

confidence: 99%

Dietary choline levels modify the effects of prenatal alcohol exposure in rats

Idrus

Breit

Thomas

2017

Neurotoxicology and Teratology

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“…It has also been established that impaired development may represent a predictive factor of behavioral modifications in adulthood (Heyser, 2004; Berk, 2006) and through the evaluation of neonatal reflexes, it is possible to identify the persistence or absence of reflexes and detect developmental delay (Schuch et al, 2016). Physical and neurobehavioral development are considered a sign of brain maturation and follow a sequence of the appearance of reflexes and maturation of motor skills (Fox, 1965; Heyser, 2004).…”

Section: Discussionmentioning

confidence: 99%

Cafeteria diet during the gestation period programs developmental and behavioral courses in the offspring

Ribeiro

Batista

Veronesi

et al. 2018

Intl J of Devlp Neuroscience

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The objective of this study was to investigate the effect of exposure to maternal consumption of a hyperenergetic, highly palatable diet, known as the cafeteria diet, during the gestation period on the development and behavior of offspring. For this, we used pregnant female mice that were fed a normal or a cafeteria diet during the gestation period. The evaluation of maternal behavior in lactating dams was performed from the second to the eighth day postpartum (PND 2-8). Weight gain, feed intake, and energy intake were recorded during the gestation period. In the offspring, reflex parameters and physical development were evaluated during the lactation period and when they reached adolescence. Behavioral performance was evaluated in light-dark, open-field, and play behavior tests. In addition, biochemical parameters of the dams and the adolescent offspring were evaluated. The cafeteria diet during gestation altered maternal behavior and the onset of physical and neurodevelopmental landmarks and had an impact on emotional and play behavior in adolescent offspring. In conclusion, our results demonstrate that exposure to maternal consumption of a cafeteria diet during the gestation period can program developmental and behavioral courses in the offspring.

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“…It was also evaluated the following reflexes over the cited days: 1) Righting reflex: the time to turn from the supine to the prone position; 2) Cliff aversion reflex: rats were placed in the edge of a table with their forelimbs overhanging. It was recorded the time to turn 90° from the edge with a maximum of 60 s; 3) Negative geotaxis: pups were placed in a inclined board (45°) with their head down and the time to turn around and climb up the board was verified (maximum of 60 s); 4) Gait: rats were placed in the center of a circle (13 cm diameter) and the time to escape off the circle with both forelimbs was recorded (maximum of 30 s) (Schuch et al, 2016; Lubics et al, 2005).…”

Section: Methodsmentioning

confidence: 99%

Gestational folic acid supplementation does not affects the maternal behavior and the early development of rats submitted to neonatal hypoxia‐ischemia but the high supplementation impairs the dam's memory and the Na⁺, K⁺ ‐ ATPase activity in the pup's hippocampus

Deniz

Confortim

Deckmann

et al. 2018

Intl J of Devlp Neuroscience

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Folic acid (FA) is a B‐complex vitamin important to the development of the fetus, being supplemented during pregnancy. Our recent findings showed that gestation supplementation (normal and excess doses) prevented the cognitive deficits and BDNF imbalance in adult rats that were submitted to neonatal hypoxia‐ischemia (HI). To better understand this protective effect, the present study aimed to evaluate whether FA supplementation could be related to (1) maternal behavior, memory and Na+, K+ ‐ ATPase activity in the hippocampus of the dams; (2) on somatic growth, early neurobehavioral development and Na+, K+ ‐ ATPase activity in the hippocampus of the offspring; and (3) the effects of this supplementation in pups submitted to neonatal HI. Pregnant Wistar rats were divided into three groups, according to the diet they received during gestation: standard diet (SD), supplemented with 2 mg/kg of FA (FA2 – normal dose) and supplemented with 20 mg/kg of FA (FA20 –excessive dose). At the 7th PND pups were submitted to the Levine‐Vannucci model of HI. During weaning the maternal behavior, the somatic growth and the neurobehavior development of pups were assessed. After weaning, the memory of the dams (by the Ox‐maze task) and the Na+, K+ ‐ ATPase activity in the hippocampus of both dams and offspring were evaluated. Considering the dams (1), both doses of FA did not alter the maternal behavior or the Na+, K+ ‐ ATPase activity in the hippocampus, but a memory deficit was observed in the high FA‐supplemented mothers. Considering the offspring (2), both FA doses did not affect the somatic growth or the neurobehavior development, but the FA20 pups had a decreased Na+, K+ ‐ ATPase activity in the hippocampus. The FA supplementation did not change the parameters evaluated in the HI rats (3) and did not prevent the decreased Na+, K+ ‐ ATPase activity in the hippocampus of the HI pups. These results indicate that normal FA supplementation dose does not influence the maternal behavior and memory and does not impact on the offspring early development in rats. Further studies are needed to confirm the effects of the high FA supplementation dose in the dams’ memory and in the Na+, K+ ‐ ATPase activity in the hippocampus of the offspring.

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Early environmental enrichment affects neurobehavioral development and prevents brain damage in rats submitted to neonatal hypoxia-ischemia

Cited by 34 publications

References 42 publications

Dietary choline levels modify the effects of prenatal alcohol exposure in rats

Dietary choline levels modify the effects of prenatal alcohol exposure in rats

Cafeteria diet during the gestation period programs developmental and behavioral courses in the offspring

Gestational folic acid supplementation does not affects the maternal behavior and the early development of rats submitted to neonatal hypoxia‐ischemia but the high supplementation impairs the dam's memory and the Na⁺, K⁺ ‐ ATPase activity in the pup's hippocampus

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Early environmental enrichment affects neurobehavioral development and prevents brain damage in rats submitted to neonatal hypoxia-ischemia

Cited by 34 publications

References 42 publications

Dietary choline levels modify the effects of prenatal alcohol exposure in rats

Dietary choline levels modify the effects of prenatal alcohol exposure in rats

Cafeteria diet during the gestation period programs developmental and behavioral courses in the offspring

Gestational folic acid supplementation does not affects the maternal behavior and the early development of rats submitted to neonatal hypoxia‐ischemia but the high supplementation impairs the dam's memory and the Na+, K+ ‐ ATPase activity in the pup's hippocampus

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Gestational folic acid supplementation does not affects the maternal behavior and the early development of rats submitted to neonatal hypoxia‐ischemia but the high supplementation impairs the dam's memory and the Na⁺, K⁺ ‐ ATPase activity in the pup's hippocampus