Early embryonic exposure of ionizing radiations disrupts zebrafish pigmentation

Wang, Yupei; Zhou, Xin; Zhao, Baofeng; Ren, Xiaotang; Chen, Yuhong; Si, Jing; Zhou, Rong; Gan, Lu; Zhang, Hong

doi:10.1002/jcp.26922

Cited by 4 publications

(4 citation statements)

References 36 publications

(55 reference statements)

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“…Therefore, PEMFs exposure appears to enhance melanin in production and transport by modulating the phosphorylation states of ERK and p38 (Kim et al, 2018). This induces MITF and DCT, promoting pigmentation in zebrafish and facilitating the establishment of a pigmentation disease model via the p-ERK/p-p38 pathway (Jin and Thibaudeau, 1999;Richardson et al, 2008;Ye et al, 2011;Liu et al, 2013;Wang et al, 2018).…”

Section: Pulsed Electromagnetic Fields (Pemfs)mentioning

confidence: 99%

Zebrafish in dermatology: a comprehensive review of their role in investigating abnormal skin pigmentation mechanisms

Qu,

Yan,

Fang

et al. 2023

Front. Physiol.

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Skin pigmentation abnormalities, ranging from aesthetic concerns to severe hyperpigmentation disease, have profound implications for individuals’ psychological and economic wellbeing. The intricate etiology of hyperpigmentation and our evolving comprehension of its underlying mechanisms underscore the need for robust animal models. Zebrafish, renowned for their transparent embryos and genetic parallels to humans, have been spotlighted as a pivotal model for skin pigmentation studies. This review offers a concise overview of zebrafish skin attributes, highlighting the shared melanin production pathways with humans. We systematically dissect the diverse strategies to craft zebrafish models of abnormal skin pigmentation, spanning physical, chemical, and genetic interventions, while critically appraising the merits and constraints of each approach. Additionally, we elucidate the metrics employed to gauge the efficacy of these models. Concluding, we cast a visionary gaze on prospective breakthroughs in the domain, aiming to steer forthcoming efforts in refined zebrafish models for skin pigmentation research.

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Section: Pulsed Electromagnetic Fields (Pemfs)mentioning

confidence: 99%

Zebrafish in dermatology: a comprehensive review of their role in investigating abnormal skin pigmentation mechanisms

Qu,

Yan,

Fang

et al. 2023

Front. Physiol.

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“…For example, orange/red and dark coloration in guppies ( Poecilia reticulata ) is affected by TPT [ 16 ]. The exposure of zebrafish embryos to ionizing radiation led to the significant loss of pigmentation [ 28 ]. After cyhalothrin-based pesticide exposure, a deeper pigmentation was observed in common carp ( Cyprinus carpio ) [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

Triphenyltin Influenced Carotenoid-Based Coloration in Coral Reef Fish, Amphiprion ocellaris, by Disrupting Carotenoid Metabolism

Zhang,

Cai,

Hou

et al. 2023

Toxics

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Triphenyltin (TPT), a kind of persistent pollutant, is prevalent in the aquatic environment and could pose a threat to coral reef fish. However, little is known about the toxicity of TPT on coral reef fish, especially regarding the representative characteristics of body coloration. Therefore, this study chose the clownfish (Amphiprion ocellaris) in order to investigate the effects of TPT exposure on its carotenoid-based body coloration under the environmentally relevant concentrations (0, 1, 10 and 100 ng/L). After TPT exposure for 60 d, the carotenoid contents were decreased and histological damage in the liver was found, shown as nuclear pyknosis and shift, lipid deposition and fibrotic tissue hyperplasia. Liver transcriptomic analysis showed that TPT exposure interfered with oxidative phosphorylation and fatty acid metabolism pathways, which related to carotenoids uptake and metabolism. Furthermore, TPT exposure led to oxidative damage in the liver, which is responsible for the changes in the antioxidant capacity of enzymes, including GSH, MDA, POD, CAT and T-SOD. TPT exposure also affected the genes (Scarb1, CD36, Stard3 and Stard5) related to carotenoid absorption and transport, as well as the genes (GstP1 and Bco2) related to carotenoid deposition and decomposition. Taken together, our results demonstrate that TPT influenced carotenoid-based coloration in coral reef fish by disrupting carotenoid metabolism, which complements the ecotoxicological effects and toxic mechanisms of TPT and provides data for the body color biology of coral reef fishes.

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“…The zebrafish (Danio rerio) is a freshwater species belonging to the minnow family (Cyprinidae, order Cypriniformes) widely used as a vertebrate research model in developmental biology, toxicology, cancer research, environmental studies and drug development due to light transparency and general ease of maintenance and breeding. Zebrafish has also been employed extensively for studies on the developmental effects on ionizing radiation [24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Protective effects of phenformin on zebrafish embryonic neurodevelopmental toxicity induced by X-ray radiation

Gan

Guo

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Radiotherapy (RT) is a common treatment for head and neck cancers, but central nervous system function can be impaired by clinical radiation doses. This experimental study evaluated the protective efficacy of the anti-hyperglycaemic/anti-neoplastic agent phenformin against radiation-induced developmental toxicity in zebrafish embryos. Zebrafish embryos pre-treated with 25 lM phenformin 1 h before x-ray irradiation were compared to irradiation-only embryos for mortality, hatching rate, morphology, spontaneous movement, heart beat, larval swimming, activities of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), malondialdehyde content (MDA, a by-product of membrane lipid oxidation), and acetylcholinesterase (AChE) activity. In addition, expression levels of multiple genes related to neural development and apoptosis (sod2, bdnf, ache, p53, bax, and bcl-2) were compared by RT-PCR and associated protein expression levels by western blotting. Pre-treatment with phenformin increased hatching rate, spontaneous movement, heart beat, and larval motor activity, decreased mortality and malformation rate, increased SOD, CAT, and AChE activities, and reduced MDA compared to irradiation-only embryos. The mRNA expression levels of anti-apoptotic sod2, bdnf, ache, and bcl-2 were enhanced while mRNA expression of p53 and pro-apoptotic bax were reduced in the phenformin pre-treatment group. Further, p53, Bax, and c-H2AX (a biomarker of DNA damage) were downregulated while Bcl-2 and BDNF were upregulated by phenformin pre-treatment. Taken together, this study supports the protective efficacy of phenformin against radiation toxicity in zebrafish embryos by suppressing oxidative stress and ensuing apoptosis.

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Early embryonic exposure of ionizing radiations disrupts zebrafish pigmentation

Cited by 4 publications

References 36 publications

Zebrafish in dermatology: a comprehensive review of their role in investigating abnormal skin pigmentation mechanisms

Zebrafish in dermatology: a comprehensive review of their role in investigating abnormal skin pigmentation mechanisms

Triphenyltin Influenced Carotenoid-Based Coloration in Coral Reef Fish, Amphiprion ocellaris, by Disrupting Carotenoid Metabolism

Protective effects of phenformin on zebrafish embryonic neurodevelopmental toxicity induced by X-ray radiation

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