Early Effects of Low Dose<sup>12</sup>C<sup>6+</sup>Ion or X-Ray Irradiation on Peripheral Blood Lymphocytes of Patients with Alimentary Tract Cancer

Chen, Yingtai; Chen, Xuezhong; Li, Yumin; Zhang, Hong; Xie, Youhua; Zhang, Xiaowei; Hui-zi, Ren; Wang, Yanling; Liao, Shiqi; He, Min; Ren, Jiangong; Zhang, Jieyu; Zhou, Xiadong; Zheng, Tongzhang; Kilfoy, Briseis A.; Zhang, Yawei

doi:10.2203/dose-response.10-015.chen

Cited by 10 publications

(8 citation statements)

References 46 publications

(64 reference statements)

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“…IFN-γ is a prominent activator of NK lymphocytes (23,24) and can exert a strong antineoplastic effect (25,26), which is not only directly cytocidal for tumor cells, but also stimulates the cytolytic functions of macrophages. It has been reported that the percentage of T-lymphocyte subsets and their protein levels in the supernatant were significantly increased at 24 h after exposure to 0.05 Gy 12 C 6+ ion irradiation (27). The findings of the current study demonstrated that, in mice exposed to priming radiation, the production of IL-1β was significantly enhanced and then gradually declined, although it remained elevated.…”

Section: Discussionsupporting

confidence: 57%

Immunological mechanism of low-dose priming radiation resistance in walker-256 tumor model mice

Feng

Qin

Guo

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. The aim of the present study was to investigate whether low-dose priming radiation induces antitumor immunity that can be augmented by the modulation of natural killer (NK) cell and cytokine activity using a mouse tumor model. Walker-256 cells were injected into the right flank of male BALB/c mice. At 7 days after inoculation, mice were divided into three groups, including group 1,2,3. In group 1 the mice were without radiation, in group 2 the mice were received 2 Gy radiation only, and in group 3 the mice were radiated with a priming dose of 75 mGy followed by 2 Gy radiation after 24 h. On day 21 following the radiation, the tumors were removed and the tumor index (tumor weight as a percentage of body weight) was calculated. At 1, 7, 14 and 21 days following the 2 Gy radiation, mouse splenocytes were isolated to analyze the NK activity and measure the production of the cytokines interleukin-1β, interferon-γ and tumor necrosis factor-α by ELISA. Apoptosis was also measured by flow cytometry. The results demonstrated that priming radiation significantly delayed the tumor growth and prolonged the median survival time to 38 days compared with the 31-day survival in the 2 Gy radiation group. The percentage of apoptotic cells was significantly higher in the mice that received 75 mGy + 2 Gy radiation compared with that in the mice that received 2 Gy alone; by contrast, mice that were not irradiated exhibited a relatively low level of apoptosis. The primed mice had a higher level of NK activity as compared with the mice exposed to 2 Gy radiation only or mice that were not irradiated. Furthermore, cytokine expression remained at a higher level in mice receiving priming dose of radiation compared that in the mice receiving only 2 Gy radiation. In conclusion, the results indicated that low-dose priming X-ray radiation may enhance the NK activity and the levels of cytokines, and that the immune response serves an important role in anticancer therapy, including radiotherapy.

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Section: Discussionsupporting

confidence: 57%

Immunological mechanism of low-dose priming radiation resistance in walker-256 tumor model mice

Feng

Qin

Guo

et al. 2017

Experimental and Therapeutic Medicine

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“…Long non-coding RNA MEG 3 has been a suggested as a biomarker for thyroid cancer and 131 I radio-resistance [50]. Proteomic studies on PTC tissue from humans have suggested NTRK1, metalloproteinases (MMP-1, MMP-9 and MMP-13) and Cathepsins (-W and -X), NF-KB and other apoptosis associated proteins as radiationinduced PTC biomarkers, together with SPANXA as an important protein for thyroid cancer development [51][52][53][54][55][56]. However, none of these were found in the present study.…”

Section: Upstreammentioning

confidence: 99%

Long-term transcriptomic and proteomic effects in Sprague Dawley rat thyroid and plasma after internal low dose 131I exposure

et al. 2020

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Background Radioiodide (131I) is commonly used to treat thyroid cancer and hyperthyroidis.131I released during nuclear accidents, have resulted in increased incidence of thyroid cancer in children. Therefore, a better understanding of underlying cellular mechanisms behind 131I exposure is of great clinical and radiation protection interest. The aim of this work was to study the long-term dose-related effects of 131I exposure in thyroid tissue and plasma in young rats and identify potential biomarkers. Materials and methods Male Sprague Dawley rats (5-week-old) were i.v. injected with 0.5, 5.0, 50 or 500 kBq 131I (Dthyroid ca 1–1000 mGy), and killed after nine months at which time the thyroid and blood samples were collected. Gene expression microarray analysis (thyroid samples) and LC-MS/MS analysis (thyroid and plasma samples) were performed to assess differential gene and protein expression profiles in treated and corresponding untreated control samples. Bioinformatics analyses were performed using the DAVID functional annotation tool and Ingenuity Pathway Analysis (IPA). The gene expression microarray data and LC-MS/MS data were validated using qRT-PCR and ELISA, respectively. Results Nine 131I exposure-related candidate biomarkers (transcripts: Afp and RT1-Bb, and proteins: ARF3, DLD, IKBKB, NONO, RAB6A, RPN2, and SLC25A5) were identified in thyroid tissue. Two dose-related protein candidate biomarkers were identified in thyroid (APRT and LDHA) and two in plasma (DSG4 and TGM3). Candidate biomarkers for thyroid function included the ACADL and SORBS2 (all activities), TPO and TG proteins (low activities). 131I exposure was shown to have a profound effect on metabolism, immune system, apoptosis and cell death. Furthermore, several signalling pathways essential for normal cellular function (actin cytoskeleton signalling, HGF signalling, NRF2-mediated oxidative stress, integrin signalling, calcium signalling) were also significantly regulated. Conclusion Exposure-related and dose-related effects on gene and protein expression generated few expression patterns useful as biomarkers for thyroid function and cancer.

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“…The average dose we applied was 45.25 mGy. In a range of studies that involved human isolated PBMCs for the investigation effect of low-dose radiation, the average dose was about 50 mGy [47,[68][69][70]. Moreover, it was noted that analysis of radiation-induced genetic damage using white blood cells can be useful to estimate doses received by people in whole-body irradiation ("accidental"), where the detection limits correspond to 100 mGy X-ray (Book "The dosimetry of ionizing radiation," page 270, by Johan J. Broerse et al, Editor Kenneth Kase).…”

Section: Discussionmentioning

confidence: 99%

A New Insight on the Radioprotective Potential of Epsilon-Aminocaproic Acid

et al. 2020

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Background and objectives: The aim of the study was to scrutinize the ability of epsilon-aminocaproic acid (EACA) to prevent radiation-induced damage to human cells. Materials and Methods: Human peripheral blood mononuclear cells (PBMCs) were exposed to ionizing radiation at three low doses (22.62 mGy, 45.27 mGy, and 67.88 mGy) in the presence of EACA at the concentration of 50 ng/mL. Results: EACA was able to prevent cell death induced by low-dose X-ray radiation and suppress the formation of reactive oxygen species (ROS). EACA also demonstrated a capacity to protect DNA from radiation-induced damage. The data indicated that EACA is capable of suppression of radiation-induced apoptosis. Comparative tests of antioxidative activity of EACA and a range of free radical scavengers showed an ability of EACA to effectively inhibit the generation of ROS. Conclusions: This study showed that the pretreatment of PBMCs with EACA is able to protect the cells from radiation-elicited damage, including free radicals’ formation, DNA damage, and apoptosis.

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Early Effects of Low Dose¹²C⁶⁺Ion or X-Ray Irradiation on Peripheral Blood Lymphocytes of Patients with Alimentary Tract Cancer

Cited by 10 publications

References 46 publications

Immunological mechanism of low-dose priming radiation resistance in walker-256 tumor model mice

Immunological mechanism of low-dose priming radiation resistance in walker-256 tumor model mice

Long-term transcriptomic and proteomic effects in Sprague Dawley rat thyroid and plasma after internal low dose 131I exposure

A New Insight on the Radioprotective Potential of Epsilon-Aminocaproic Acid

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Early Effects of Low Dose12C6+Ion or X-Ray Irradiation on Peripheral Blood Lymphocytes of Patients with Alimentary Tract Cancer

Cited by 10 publications

References 46 publications

Immunological mechanism of low-dose priming radiation resistance in walker-256 tumor model mice

Immunological mechanism of low-dose priming radiation resistance in walker-256 tumor model mice

Long-term transcriptomic and proteomic effects in Sprague Dawley rat thyroid and plasma after internal low dose 131I exposure

A New Insight on the Radioprotective Potential of Epsilon-Aminocaproic Acid

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Early Effects of Low Dose¹²C⁶⁺Ion or X-Ray Irradiation on Peripheral Blood Lymphocytes of Patients with Alimentary Tract Cancer