Early effects of carbon-ion irradiation on murine lymphocytes and thymocytes

Xie, Youhua; Zhang, Hong; Bingrong, Dang; Bing, Tao; Jiang, Hao; Guo, Hongyun; Wang, Xiaohu

doi:10.1016/j.asr.2007.04.092

Cited by 1 publication

(1 citation statement)

References 19 publications

(19 reference statements)

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“…every day, then the Low-TP (20 mg/kg·BW), Middle-TP (40 mg/kg·BW) and High-TP (80 mg/kg·BW) were administered with the corresponding concentration TP by i.p., and the work lasted 10 days ( Figure 1 ). 12 h after the last i.p., we measured body weight of each mouse, and then collected blood from the retro-orbital axis while under anesthesia and immediately took the liver, thymus and spleen after that mice were killed and weighted thymus and spleen to calculate immune organ index [ 23 ] (thymus index = thymus weight (mg) × 10/body weight (g), spleen index = spleen weight (mg) × 10/body weight (g)). Blood serum was collected with 3000 r/min for 15 min at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

Immunomodulatory Effect of Tremella Polysaccharides against Cyclophosphamide-Induced Immunosuppression in Mice

Zhou

Chen

et al. 2018

Molecules

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Polysaccharides are closely associated with immune regulation, but there are different polysaccharide effects from different sources. In this study, the aim was to investigate the effect of tremella polysaccharides (TP) in cyclophosphamide-induced immunodeficient mice. We observed the thymus and spleen index, liver and spleen pathological changes, and the levels of IL-2, IL-12, INF-γ, TGF-β and Ig G in serum, and we also noted the mRNA expression of IL-1β, IL-4, IL-12 and TGF-β in liver and spleen. Besides, we also measured the best effects of different doses of TP (Low-TP was 20 mg/kg·BW, Middle-TP was 40 mg/kg·BW, and High-TP was 80 mg/kg·BW) on cyclophosphamide-induced immunosuppressed mice. The results were remarkable, and suggested that TP had a significant effect for enhancing immunity in cyclophosphamide-induced immunosuppression, and the immune enhancement of High-TP had the best results in TP-treated mice. It could significantly increase the thymus and spleen index, alleviate pathological features of immunosuppression such as the arrangement of liver sinusoid and hepatic plates was disordered, massive inflammatory cells infiltrated and fatty degeneration of hepatocytes in liver, and red pulp and white pulp were intermixed, splenic corpuscles demolished and disappeared, splenic sinusoid extended, and lymphocytes of spleen were reduced in spleen. Besides, it could also up-regulate serum levels of IL-2, IL-12, INF-γ and Ig G, reduce the level of TGF-β in serum, markedly promote mRNA expression of IL-1β, IL-4 and IL-12 in liver and spleen, and suppress mRNA expression of TGF-β. Above all, TP showed preventive effect for cyclophosphamide-induced immunosuppressed mice.

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Section: Methodsmentioning

confidence: 99%