Early Diagnosis of Acute Myocardial Infarction Using Immunosensors and Immunotests

Chan, Cangel Pui-yee; Cheng, Wendy; Glatz, Jan F. C.; Voort, D. van der; Sanderson, John E.; Hempel, Albrecht; Lehmann, Matthias; Renneberg, Ilka; Renneberg, Reinhard

doi:10.1081/al-120023625

Cited by 22 publications

(7 citation statements)

References 27 publications

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“…Analyses were performed with the SAS software package V8.2 (SAS Institute, Cary, NC). The sample size was determined according to our primary objective (comparison of sensitivity) and to previous studies which has evaluated the diagnostic performance of the CardioDetect® assay as compared to conventional biomarkers of myonecrosis [16,17].…”

Section: Study End Point and Statistical Analysismentioning

confidence: 99%

Pre-hospital detection of acute myocardial infarction with ultra-rapid human fatty acid-binding protein (H-FABP) immunoassay

Ecollan

Collet

Boon

et al. 2007

International Journal of Cardiology

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Section: Study End Point and Statistical Analysismentioning

confidence: 99%

Pre-hospital detection of acute myocardial infarction with ultra-rapid human fatty acid-binding protein (H-FABP) immunoassay

Ecollan

Collet

Boon

et al. 2007

International Journal of Cardiology

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“…A one-step H-FABP immunotest so-called CardioDetect® designed to detect H-FABP in whole blood samples is now commercially available [4][5][6]. The test result is available within 15 min after addition of blood samples.…”

Section: Introductionmentioning

confidence: 99%

Human heart-type fatty acid-binding protein for on-site diagnosis of early acute myocardial infarction

Liao

Chan

Cheung

et al. 2009

International Journal of Cardiology

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“…The immunoassay is one the most important analytical methods for clinical diagnosis and biochemical studies because of its extremely high specificity (Chan et al 2003). Of the various solid-phase immunoassay formats in current use, the sandwich immunoassay typically carried out in polystyrene microtiter plates is superior to other types of heterogeneous solid phase immunoassays with respect to sensitivity, specificity and kinetics (Diamandis and Christopoulos 1996).…”

Section: Introductionmentioning

confidence: 99%

Antibody immobilization on to polystyrene substrate—on-chip immunoassay for horse IgG based on fluorescence

Darain

Gan

Tjin

2009

Biomed Microdevices

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A simple microfluidic immunoassay card was developed based on polystyrene (PS) substrate for the detection of horse IgG, an inexpensive model analyte using fluorescence microscope. The primary antibody was captured onto the PS based on covalent bonding via a self-assembled monolayer (SAM) of thiol to pattern the surface chemistry on a gold-coated PS. The immunosensor chip layers were fabricated from sheets by CO(2) laser ablation. The functionalized PS surfaces after each step were characterized by contact angle measurement, X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM). After the antibody-antigen interaction as a sandwich immunoassay with a fluorescein isothiocyanate (FITC)-conjugated secondary antibody, the intensity of fluorescence was measured on-chip to determine the concentration of the target analyte. The present immunosensor chip showed a linear response range for horse IgG between 1 microg/ml and 80 microg/ml (r = 0.971, n = 3). The detection limit was found to be 0.71 microg/ml. The developed microfluidic system can be extended for various applications including medical diagnostics, microarray detection and observing protein-protein interactions.

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Early Diagnosis of Acute Myocardial Infarction Using Immunosensors and Immunotests

Cited by 22 publications

References 27 publications

Pre-hospital detection of acute myocardial infarction with ultra-rapid human fatty acid-binding protein (H-FABP) immunoassay

Pre-hospital detection of acute myocardial infarction with ultra-rapid human fatty acid-binding protein (H-FABP) immunoassay

Human heart-type fatty acid-binding protein for on-site diagnosis of early acute myocardial infarction

Antibody immobilization on to polystyrene substrate—on-chip immunoassay for horse IgG based on fluorescence

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