Early development of human hematopoietic and acquired immune systems in new born NOD/Scid/Jak3null mice intrahepatic engrafted with cord blood-derived CD34+ cells

Abstract

An animal model in which the human immune system can be reconstituted is necessary to study acquired immunity in vivo. We report here a novel model, the NOD/SCID/JAK3(null) mouse, for the human immune system's development. Newborn mice transplanted with human cord blood CD34(+) cells intrahepatically, developed human T and B cells, and myeloid and plasmacytoid dendritic cells. The T and B cells had a naïve to memory phenotype, and included plasma cells. The human acquired immune system can be reconstituted from CD34(+) cells in NOD/SCID/JAK3(null) mice. This model is a powerful tool for the study of human immunity.

Establishment of a Patient-Derived Tumor Xenograft Model and Application for Precision Cancer Medicine

“…NOD/SCID mice have the advantages of accepting normal and malignant human hematopoietic cells with high efficiency as well as human solid tumors. 8) Recently, NOG (NOD/Scid/ IL2Rγ null ), 9) NSG (NOD/Scid/IL2Rγ null ), 10) and NOJ (NOD/ Scid/Jak3 null ) 11) mice with complete loss of NK cells have been established based on NOD/SCID mice (Figs. 2, 3).…”

Current advances in humanized mouse models

“…25 To overcome this issue, various improved strains based on these immunodeficient mice have been developed or are being developed by several groups, including our group. 34,35 In Figure 1, the history of the development of immunodeficient humanized mice and their respective improvements are summarized. These mice have been established in NOG/NSG or BRG backgrounds.…”

22‐Oxa‐1,25‐dihydroxyvitamin D3 efficiently inhibits tumor growth in inoculated mice and primary histoculutre of cholangiocarcinoma

“…15 NOJ male mice (ages 8-10 weeks) were housed and monitored in the animal research facility according to institutional guidelines. All experimental procedures and protocols were approved by the Institutional Animal Care and Use Committee at Kumamoto University.…”