Early detection of Schistosoma mansoni infection by touchdown PCR in a mouse model

Suzuki, Tomoyuki; Osada, Yoshio; Kumagai, Toru; Hamada, Atsuo; Okuzawa, Eiichi; Kanazawa, Tamotsu

doi:10.1016/j.parint.2006.05.004

Cited by 35 publications

(31 citation statements)

References 34 publications

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“…Indeed, PCR identification of S. mansoni in urine sediments has proved superior in diagnostic accuracy to the KK and urine CCA tests in areas of endemicity (224). PCR-based tests can detect CFPD in host serum from a very early schistosome infection (225,226), even in the first week postinfection (227), thus representing a useful adjunct for the early diagnosis of schistosomiasis. In combination with real-time PCR, the approach has proven valuable for monitoring therapeutic responses (222,228), as the amount of CFPD declines gradually following effective treatment.…”

Section: Detection Of Cell-free Parasite Dna In Serum and Other Body mentioning

confidence: 99%

Advances in the Diagnosis of Human Schistosomiasis

et al. 2015

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SUMMARY Schistosomiasis is a major neglected tropical disease that afflicts more than 240 million people, including many children and young adults, in the tropics and subtropics. The disease is characterized by chronic infections with significant residual morbidity and is of considerable public health importance, with substantial socioeconomic impacts on impoverished communities. Morbidity reduction and eventual elimination through integrated intervention measures are the focuses of current schistosomiasis control programs. Precise diagnosis of schistosome infections, in both mammalian and snail intermediate hosts, will play a pivotal role in achieving these goals. Nevertheless, despite extensive efforts over several decades, the search for sensitive and specific diagnostics for schistosomiasis is ongoing. Here we review the area, paying attention to earlier approaches but emphasizing recent developments in the search for new diagnostics for schistosomiasis with practical applications in the research laboratory, the clinic, and the field. Careful and rigorous validation of these assays and their cost-effectiveness will be needed, however, prior to their adoption in support of policy decisions for national public health programs aimed at the control and elimination of schistosomiasis.

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Section: Detection Of Cell-free Parasite Dna In Serum and Other Body mentioning

confidence: 99%

Advances in the Diagnosis of Human Schistosomiasis

et al. 2015

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“…However, this indicates that false-positive results might have been observed due to cross-reactivity with other helminth infections but this can be ruled, since the DNA from these worms is not amplified by the S. mansonispecific primers [10,12].…”

Section: Discussionmentioning

confidence: 99%

“…Using schistosome genome specific primers, allowed detection of the parasite DNA by polymerase chain reaction (PCR) in biological samples from infected subjects with superior specificity and sensitivity than the parasitological or the serological methods [7][8][9][10][11][12][13][14], as demonstrated by the capacity of the PCR to detect parasite DNA as early as 7 days post infection. While, the levels of the anti-worm IgG remained high at 23 weeks post-treatment, the PCR results turned negative at week 10 posttreatment [14].…”

Section: Introductionmentioning

confidence: 99%

Comparative sensitivities of various tests for diagnosing early Schistosoma mansoni infection in mice

Bahgat¹,

Ibrahim²,

Maghraby³

et al. 2011

J Bacteriol Parasitol

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Objective: We compared the diagnostic values of cercarial antigen preparation, cercarial secretions, soluble worm antigen preparation and worm vomit prepared from the parasite Schistosoma mansoni.Methods: Enzyme linked immunosorbant assay was used to detect IgG in plasma from Schistosoma mansoni infected mice. In parallel, specific primers for the parasite genome was used to detect S. mansoni DNA in plasma and urine from infected mice and hemolymph and tissues of infected Biomphalaria alexandrina snails by Polymerase chain reaction. Results:The results showed that all the above diagnostic approaches enabled infection to be diagnosed as early as three days post mice exposure to parasite cercariae. Conclusion:Cercarial secretions and worm vomit represent new useful economic crude antigens for preliminary detection of parasite active transmission or response to therapy in an endemic setting. Also, it was found that the detection of Schistosoma mansoni DNA in urine from infected mice was the most sensitive and specific (although expensive) method for infection diagnosis than all the others. Comparative sensitivities of various tests for diagnosing early Schistosoma mansoni infection in mice Journal of Bacteriology and ParasitologyCitation: Bahgat MM, Ibrahim AM, Maghraby AS, Rizk M, Megeed RA (2011) Comparative sensitivities of various tests for diagnosing early Schistosoma mansoni infection in mice. J Bacteriol Parasitol 2:116.

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“…The first primer targeted to the 97-bp repeated DNA sequence, DraI, which is specific to S. haematobium (10). The second primer targeted to the 121-bp tandem repeated DNA sequence, which is specific to S. mansoni (11,25). These specific DNA sequences are not contained in the DNA of S. japonicum.…”

Section: Case Reportmentioning

confidence: 99%

Cerebral Schistosomiasis Due to Schistosoma haematobium Confirmed by PCR Analysis of Brain Specimen

et al. 2011

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The case of a 25-year-old Japanese male who had cerebral schistosomiasis caused by Schistosoma haematobium is reported here. Although serum antibody tests showed a cross-reaction with other helminths and no ova were excreted in urine or feces, the existence of Schistosoma haematobium in the brain was confirmed by PCR analysis. CASE REPORTIn October 2009, a 25-year-old Japanese man was admitted to a local community hospital in Japan with a 1-week history of mild headache and sporadic paraphasia. He had worked as an agricultural consultant in the Republic of Malawi from April 2007 to June 2009. During his stay, he lived with local residents, consumed water from a well, and had swum in a lake at least twice. He had been in excellent health until October 2009, except for a Giardia lamblia infection in 2008. At the community hospital, a computed tomography (CT) scan of the patient's head showed four hyperdense and edematous lesions in the left parietal lobe, and these lesions were suspected to be related to tropical infectious diseases due to the fact that the onset of his symptoms appeared soon after his return from the Republic of Malawi. Subsequently, the patient was referred to our institution for further workup.Upon presentation to our institute, the patient's temperature was 36.8°C, his pulse was 60 beats per minute (bpm), and his blood pressure was 120/70 mm Hg. Although the patient was alert and appropriate at a glance, verbal paraphasia was occasionally observed. Laboratory evaluation revealed the following: white blood cell count, 8,780/l (67.5% neutrophils, 25.0% lymphocytes, 1.5% eosinophils); serum C-reactive protein, 0.03 mg/dl; IgE, 18 U/ml; HIV antibody negative; toxoplasma IgM and IgG negative; and Entamoeba antibody negative. A magnetic resonance imaging (MRI) scan of the brain with gadolinium enhancement showed a couple of ill-defined, heterogeneously enhancing lesions. They were each approximately 10 mm in diameter, in the left parietal lobe, with increased intensity of the signal on the T1-weighted image (Fig. 1). A lumbar puncture was not performed. The patient's headache and nausea worsened rapidly, and we were obliged to relieve his symptoms as soon as possible. Based on the clinical presentation and characteristic imaging finding, we clinically concluded that the cerebral lesions were neurocysticercosis. Albendazole (15 mg/kg of body weight per day) was administered with dexamethasone (0.1 mg/kg per day) for a total of 8 days. The patient's headache and nausea then subsided, and the verbal paraphasia disappeared. The findings from an MRI scan of the brain were improved but still remained.One week after the initiation of treatment, the results of the commercially available serum enzyme-linked immunosorbent assay (ELISA; SRL, Tokyo, Japan), which can detect IgG antibody for 12 helminthic diseases as a screening (22), were reported: Spirometra erinacei (also known as Spirometra mansoni) antibody on admission was positive, whereas Taenia solium antibody was negative. Schistosoma species are not ...

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Early detection of Schistosoma mansoni infection by touchdown PCR in a mouse model

Cited by 35 publications

References 34 publications

Advances in the Diagnosis of Human Schistosomiasis

Advances in the Diagnosis of Human Schistosomiasis

Comparative sensitivities of various tests for diagnosing early Schistosoma mansoni infection in mice

Cerebral Schistosomiasis Due to Schistosoma haematobium Confirmed by PCR Analysis of Brain Specimen

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