Early detection of lung cancer biomarkers through biosensor technology: A review

Roointan, Amir; Mir, Tanveer Ahmad; Wani, Shadil Ibrahim; Mati-ur-Rehman,; Hussain, Khalil K.; Ahmed, Bilal; Abrahim, Shugufta; Savardashtaki, Amir; Gandomani, Ghazaal; Gandomani, Molood; Chinnappan, Raja; Akhtar, Mahmood Hassan

doi:10.1016/j.jpba.2018.10.017

Cited by 140 publications

(80 citation statements)

References 121 publications

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“…Lung cancer is classified histologically into two types: small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) [2,5,7,8]. The overall 5-year survival rate of lung cancer, including SCLC and NSCLC, is the lowest (18%) among all cancers [9]. In Taiwan, lung cancer is also the leading cancer death, where about 39.2 individuals per 100,000 die annually from lung cancer according to the report of the Department of Health, R.O.C.…”

Section: Introductionmentioning

confidence: 99%

Casticin Induces DNA Damage and Affects DNA Repair Associated Protein Expression in Human Lung Cancer A549 Cells

et al. 2020

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Casticin was obtained from natural plants, and it has been shown to exert biological functions; however, no report concerns the induction of DNA damage and repair in human lung cancer cells. The objective of this study was to investigate the effects and molecular mechanism of casticin on DNA damage and repair in human lung cancer A549 cells. Cell viability was determined by flow cytometric assay. The DNA damage was evaluated by 4’,6-diamidino-2-phenylindole (DAPI) staining and electrophoresis which included comet assay and DNA gel electrophoresis. The protein levels associated with DNA damage and repair were analyzed by western blotting. The expression and translocation of p-H2A.X were observed by confocal laser microscopy. Casticin reduced total viable cell number and induced DNA condensation, fragmentation, and damage in A549 cells. Furthermore, casticin increased p-ATM at 6 h and increased p-ATR and BRCA1 at 6–24 h treatment but decreased p-ATM at 24–48 h, as well as decreased p-ATR and BRCA1 at 48 h. Furthermore, casticin decreased p-p53 at 6–24 h but increased at 48 h. Casticin increased p-H2A.X and MDC1 at 6–48 h treatment. In addition, casticin increased PARP (cleavage) at 6, 24, and 48 h treatment, DNA-PKcs and MGMT at 48 h in A549 cells. Casticin induced the expressions and nuclear translocation of p-H2AX in A549 cells by confocal laser microscopy. Casticin reduced cell number through DNA damage and condensation in human lung cancer A549 cells.

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Section: Introductionmentioning

confidence: 99%

Casticin Induces DNA Damage and Affects DNA Repair Associated Protein Expression in Human Lung Cancer A549 Cells

et al. 2020

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“…Biosensors can be mainly classified as optical, electrochemical, and mass spectrometric. Among biosensors, electrochemical biosensors with a volumetric transducer displayed a great potential in the detection of biomolecules [112]. Electrochemical biosensors are widely used for the detection of various analytes because of their rapid response, great sensitivity, simplicity, cost-effective, miniaturization, and portable.…”

Section: Methodsmentioning

confidence: 99%

Electrochemical virus detections with nanobiosensors

Kaya

Karadurmuş

Özçelikay

et al. 2020

Nanosensors for Smart Cities

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“…The AuNSs (core diameter = 27 nm, branch length = 12 nm, tip diameter = 9 nm) coupled with carboxyl functionalisation of the GO film (thickness = 0.963 nm) reduced the LOD 32 times in comparison to an unenhanced GO-based sensor. Another interesting strategy used in developing SPR sensors is the use of molecularly imprinted polymers SPR sensors have also been widely explored for early stage cancer diagnosis [43][44][45][46][47][48][49]. They have been used for the detection of prominent cancer biomarkers such as cytokeratins [50][51][52], prostate specific antigen (PSA) [53][54][55], nucleic acids [56] and several other such molecules [57][58][59][60][61][62].…”

Section: Surface Plasmon Resonance Sensors For Biomedical Diagnosticsmentioning

confidence: 99%

Recent Progress in Optical Sensors for Biomedical Diagnostics

Pirzada

Altıntaş

2020

Micromachines

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In recent years, several types of optical sensors have been probed for their aptitude in healthcare biosensing, making their applications in biomedical diagnostics a rapidly evolving subject. Optical sensors show versatility amongst different receptor types and even permit the integration of different detection mechanisms. Such conjugated sensing platforms facilitate the exploitation of their neoteric synergistic characteristics for sensor fabrication. This paper covers nearly 250 research articles since 2016 representing the emerging interest in rapid, reproducible and ultrasensitive assays in clinical analysis. Therefore, we present an elaborate review of biomedical diagnostics with the help of optical sensors working on varied principles such as surface plasmon resonance, localised surface plasmon resonance, evanescent wave fluorescence, bioluminescence and several others. These sensors are capable of investigating toxins, proteins, pathogens, disease biomarkers and whole cells in varied sensing media ranging from water to buffer to more complex environments such as serum, blood or urine. Hence, the recent trends discussed in this review hold enormous potential for the widespread use of optical sensors in early-stage disease prediction and point-of-care testing devices.

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Early detection of lung cancer biomarkers through biosensor technology: A review

Cited by 140 publications

References 121 publications

Casticin Induces DNA Damage and Affects DNA Repair Associated Protein Expression in Human Lung Cancer A549 Cells

Casticin Induces DNA Damage and Affects DNA Repair Associated Protein Expression in Human Lung Cancer A549 Cells

Electrochemical virus detections with nanobiosensors

Recent Progress in Optical Sensors for Biomedical Diagnostics

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