Early detection of Alzheimer's disease using a biosensor based on electrochemically-reduced graphene oxide and gold nanowires for the quantification of serum microRNA-137

Azimzadeh, Mostafa; Nasirizadeh, Navid; Rahaie, Mahdi; Naderi-Manesh, Hossein

doi:10.1039/c7ra09767k

Cited by 92 publications

(61 citation statements)

References 90 publications

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“…Another example of miRNA detection is the work performed by Azimzaeh et al [ 87 ] for the detection of miRNA-137 using a screen-printed carbon electrode (SPCE) modified with electrochemically-reduced GO and gold nanowires for enhancing the sensitivity of the biosensor and facilitating the immobilization of single stranded DNA (ssDNA) probe ( Figure 2 B). As electrochemical tag, doxorubicin was employed, due to its capacity to get intercalated in double stranded oligonucleotides [ 88 ] and also its electroactive properties, so that correlating directly to the concentration of hybridized biomarker.…”

Section: Electrochemical Biosensors For Alzheimer’s Disease Diagnomentioning

confidence: 99%

“…Schematic representation of miRNA biosensor based on the modification of a SPCE using GO, gold nanowires, thiolated RNA probe as recognition element and doxorubicin as reporter signal due to its intercalating capacity (top) and DPV curves of decreasing concentrations of target miRNA and the corresponding calibration curve (bottom). Reproduced from [ 87 ]—Published by The Royal Society of Chemistry.…”

Section: Figurementioning

confidence: 99%

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Electrochemical Biosensors Based on Nanomaterials for Early Detection of Alzheimer’s Disease

Toyos-Rodríguez

Alonso

Escosura‐Muñiz

2020

Sensors

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Alzheimer’s disease (AD) is an untreatable neurodegenerative disease that initially manifests as difficulty to remember recent events and gradually progresses to cognitive impairment. The incidence of AD is growing yearly as life expectancy increases, thus early detection is essential to ensure a better quality of life for diagnosed patients. To reach that purpose, electrochemical biosensing has emerged as a cost-effective alternative to traditional diagnostic techniques, due to its high sensitivity and selectivity. Of special relevance is the incorporation of nanomaterials in biosensors, as they contribute to enhance electron transfer while promoting the immobilization of biological recognition elements. Moreover, nanomaterials have also been employed as labels, due to their unique electroactive and electrocatalytic properties. The aim of this review is to add value in the advances achieved in the detection of AD biomarkers, the strategies followed for the incorporation of nanomaterials and its effect in biosensors performance.

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Section: Electrochemical Biosensors For Alzheimer’s Disease Diagnomentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Electrochemical Biosensors Based on Nanomaterials for Early Detection of Alzheimer’s Disease

Toyos-Rodríguez

Alonso

Escosura‐Muñiz

2020

Sensors

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“…Biosensors are analytical devices that convert biological responses into electrical, optical, or mass-sensitive signals. Biosensors use output elements to obtain the quantity of the target molecules [11]. Over time, biosensors have increased in importance due to advantages, such as rapid real-time detection, portability, ease of use, and multi-pathogen detection in field and laboratory analyses [12].…”

Section: Introductionmentioning

confidence: 99%

Glycoprotein- and Lectin-Based Approaches for Detection of Pathogens

et al. 2020

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Infectious diseases alone are estimated to result in approximately 40% of the 50 million total annual deaths globally. The importance of basic research in the control of emerging and re-emerging diseases cannot be overemphasized. However, new nanotechnology-based methodologies exploiting unique surface-located glycoproteins or their patterns can be exploited to detect pathogens at the point of use or on-site with high specificity and sensitivity. These technologies will, therefore, affect our ability in the future to more accurately assess risk. The critical challenge is making these new methodologies cost-effective, as well as simple to use, for the diagnostics industry and public healthcare providers. Miniaturization of biochemical assays in lab-on-a-chip devices has emerged as a promising tool. Miniaturization has the potential to shape modern biotechnology and how point-of-care testing of infectious diseases will be performed by developing smart microdevices that require minute amounts of sample and reagents and are cost-effective, robust, and sensitive and specific. The current review provides a short overview of some of the futuristic approaches using simple molecular interactions between glycoproteins and glycoprotein-binding molecules for the efficient and rapid detection of various pathogens at the point of use, advancing the emerging field of glyconanodiagnostics.

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“…Speci cally, for exible chemo resistive sensors.Au nanowires (AuNWs) are attractive because they possess minimal cross-sectional area but have the ability to increase the oodcurrent along the axial current direction, resulting in higher conductance changes compared to the typical zero-dimensional (0-D) nanomaterials [44,45]. The chemoreceptive characteristics of AuNWs such as the capability to serve as biocompatible surface for the immobilisation of biomolecules in a correct orientation which enhances the binding a nity of antigen-antibody and the ability to support a swift electron-transfer which causes the ow of one-way electron between two electrodes are key considerations in designing the MC nano biosensor [46][47][48][49][50][51]. Chemoreceptive and also conductometric characteristics played by the AuNWs are both important for biosensor transduction mechanism.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, Characterisation and Cytotoxicity of Gold Nanowires for Ultra-Sensitive Biosensor Development

Lah

Gray

Trigueros

2020

Preprint

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With the long-term goal of developing an ultra-sensitive microcantilever-based nano biosensor for versatile biomarker detection, new controlled bioreceptor-analytes systems are being explored to overcome the disadvantages of conventional ones. Gold nanowires (AuNWs) have been used as a probe to overcome the tolerance problem that occurs in response to changes in environmental conditions. However, the cytotoxicity of AuNWs is still unclear. Here, we examined the cytotoxicity of AuNWs systems using both commercial and as-synthesised AuNWs. In vitro experiments show that commercial AuNWs with an average quoted length of 6 µm are highly toxic against Gram-negative Escherichia coli (E. coli) at 50 µg/ml. However, this toxicity is due to the presence of CTAB surfactant not by the nanostructure. Conversely, the as-synthesised AuNWs with an average length of 9.5 µm show non-cytotoxicity even at the maximum viable concentration (330µg/ml). These findings may lead to the development of potentially life-saving cytotoxicity -free nano biosensors for an early diagnostic of potential diseases.

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Early detection of Alzheimer's disease using a biosensor based on electrochemically-reduced graphene oxide and gold nanowires for the quantification of serum microRNA-137

Abstract: Serum miR-137 is quantified for the early detection of Alzheimer's disease using a electrochemically reduced graphene oxide and gold nanowire modified electrode.

Cited by 92 publications

References 90 publications

Electrochemical Biosensors Based on Nanomaterials for Early Detection of Alzheimer’s Disease

Electrochemical Biosensors Based on Nanomaterials for Early Detection of Alzheimer’s Disease

Glycoprotein- and Lectin-Based Approaches for Detection of Pathogens

Synthesis, Characterisation and Cytotoxicity of Gold Nanowires for Ultra-Sensitive Biosensor Development

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