Early dengue virus interactions: the role of dendritic cells during infection

Souza, Higo Fernando Santos; Almeida, Bianca da Silva; Boscardin, Sílvia Beatriz

doi:10.1016/j.virusres.2016.07.001

Cited by 10 publications

(10 citation statements)

References 126 publications

(133 reference statements)

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“…Therefore, the autopsy studies do not indicate the tropism of DENV in major cases of DENV infection. (2) The contradictory results obtained during the tropism studies of various organs (brain, thymus, heart, kidney, GI tract and bone marrow) can be explained depending on the fact that the severity of DENV infection depends on multiple factors such as virus strain and its virulence, age of the individual, sex, high BMI, immune status and host genetics [1,[5][6][7]. Similarly, the above factors along with the sample preparation technique, and the stage (primary or secondary) and severity (DF, DHF or DSS) of the infection when the patient died may also contribute to DENV tropism.…”

Section: Discussionmentioning

confidence: 99%

“…DENV is an enveloped virus, with a virion size of 50nm, and contains a positive-sense single-stranded RNA of approximately 11kb in size, packaged by viral capsid (C) proteins. The genome has one open reading frame (ORF) and encodes a single polyprotein that is further processed to generate three structural proteins, namely capsid(C), pre-membrane/membrane (prM/M) and envelope (E), and seven non-structural proteins, namely NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5 [1]. The envelope of the virus is a host-derived lipid bilayer, surrounded by 180 copies of two different glycoproteins (prM and E) [2].…”

Section: Introductionmentioning

confidence: 99%

“…DENV infection is endemic in hot climatic regions of the world and according to WHO 2017 reports, an estimated 390 million DENV infection cases per year have been reported-of which, 96 million cases display the symptoms of the disease [4]. The acuteness of the infection depends on various factors such as virus strain and its virulence, age of the individual, sex, high BMI, immune status and host genetics [1,[5][6][7]. It leads to asymptomatic infection in the majority of cases but, in some circumstances, it may lead to self-healing, mild flu-like symptoms known as Dengue fever (DF) or other severe forms of the disease such as Dengue hemorrhagic fever (DHF).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Insight into the Tropism of Dengue Virus in Humans

Begum

Das

Mukherjee

et al. 2019

Viruses

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In tropical and subtropical zones, arboviruses are among the major threats to human life, affecting a large number of populations with serious diseases. Worldwide, over three hundred million people are infected with dengue virus (DENV) every year as per the World Health Organization (WHO). DENV-mediated disease severity ranges from a mild fever to hemorrhagic fever and shock syndrome. Patients suffering from severe infection might experience multi-organ failure, cardiomyopathy and even encephalopathy, further complicating the disease pathogenesis. In life-threatening cases, DENV has been reported to affect almost all organs of the human body. In this review, we discuss the organ tropism of DENV in humans in depth as detected in various autopsy studies. Keeping in mind the fact that there is currently no DENV-specific antiviral, it is of utmost importance to achieve a vivid picture of the susceptible cells in humans which might help in designing antivirals against DENV, especially targeting those tissues in which infection might lead to life-threatening conditions.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Insight into the Tropism of Dengue Virus in Humans

Begum

Das

Mukherjee

et al. 2019

Viruses

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“…Five distinct serotypes of DENV, a single-stranded RNA arbovirus, has been identified, and all cause dengue fever. Typically, DENV infection induces multiple hematologic abnormalities, including leukopenia, neutropenia, and thrombocytopenia ( 59 ). BM biopsies isolated during DENV infection are characterized by abnormal megakaryopoiesis, reticulocytopenia, and granulocytopenia ( 60 , 61 ).…”

Section: Dengue Virus (Denv)-induced Bm Failurementioning

confidence: 99%

Microbe-Induced Inflammatory Signals Triggering Acquired Bone Marrow Failure Syndromes

2017

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Acquired bone marrow failure syndromes encompass a unique set of disorders characterized by a reduction in the effective production of mature cells by the bone marrow (BM). In the majority of cases, these syndromes are the result of the immune-mediated destruction of hematopoietic stem cells or their progenitors at various stages of differentiation. Microbial infection has also been associated with hematopoietic stem cell injury and may lead to associated transient or persistent BM failure, and recent evidence has highlighted the potential impact of commensal microbes and their metabolites on hematopoiesis. We summarize the interactions between microorganisms and the host immune system and emphasize how they may impact the development of acquired BM failure.

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“…Although the small volume of initially injected mosquito saliva contains fewer than 100 plaque forming units (PFU) of DENV [12], when the DENV becomes amplified, various clinical symptoms develop [13]. Therefore, as the initial target cells of DENV, DCs are critical for primary replication in the human epidermis/dermis during the early infection phase [14,15]. Moreover, another group has demonstrated that primary DCs showed ADE in DENV infection [16].…”

Section: Introductionmentioning

confidence: 99%

Dengue virus susceptibility in novel immortalized myeloid cells

Yamanaka

Miyazaki²,

Shimizu³

et al. 2020

Heliyon

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Human dendritic cells (DCs) are the main target cells of dengue virus (DENV). Because humans injected with even a small volume of DENV from mosquito saliva display a high level of viremia, DCs are expected to be highly susceptible to DENV. In the present study, we assessed the efficiency of DENV infection using the novel immortalized human myeloid cell lines iPS-ML and iPS-DC. To prepare the DC-like myeloid cell line (iPS-DC), iPS-ML cells were cultured in the presence of IL-4 for 72 h. iPS-DC cells were the most susceptible to DENV, followed by iPS-ML, Vero and K562 cells. In contrast, the highest infective yield titer was observed in Vero cells. To investigate further uses of iPS-ML and iPS-DC, these cells were applied to an assay measuring antibody-dependent enhancement (ADE) activity in DENV infection. Serum samples collected from healthy Thai participants and mouse monoclonal antibodies displayed similar ADE activity patterns when examined with iPS-ML, iPS-DC, or K562 cells, the last of which are usually used in conventional ADE assays. Interestingly, iPS-ML cells showed greater susceptibility to ADE activity than iPS-DC and K562 cells. Here, we demonstrated the potential utility of the novel immortalized human myeloid cell lines iPS-ML and iPS-DC in future research on DENV.

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Early dengue virus interactions: the role of dendritic cells during infection

Cited by 10 publications

References 126 publications

Insight into the Tropism of Dengue Virus in Humans

Insight into the Tropism of Dengue Virus in Humans

Microbe-Induced Inflammatory Signals Triggering Acquired Bone Marrow Failure Syndromes

Dengue virus susceptibility in novel immortalized myeloid cells

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