Early Checking of Genetic Stability of Cork Oak Somatic Embryos by AFLP Analysis

Hornero, Javier; Martinez, I.; Celestino, C.; Gallego, Francisco Javier; Torres, Vicente E.; Toribio, M.

doi:10.1086/320784

Cited by 33 publications

(27 citation statements)

References 31 publications

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“…Some genetic molecular markers, such as RFLP (DeVerno et al 1994), RAPD (Isabel et al 1993;Fourré et al 1997) and AFLP (Hornero et al 2001), have been used for analysis of true-to-typeness. Microsatellites can serve as highly sensitive markers for monitoring genetic variation that may signal potential deleterious mutations during in vitro culture, because they reflect a relatively high rate of mutation and corresponding degree of genetic variability (Burg et al 1999;Leroy et al 2000;Wilhelm et al 2005;Lopes et al 2006;Burg et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Analysis of genetic stability at SSR loci during somatic embryogenesis in maritime pine (Pinus pinaster)

et al. 2009

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Somatic embryogenesis (SE) is a propagation tool of particular interest for accelerating the deployment of new high-performance planting stock in multivarietal forestry. However, genetic conformity in in vitro propagated plants should be assessed as early as possible, especially in long-living trees such as conifers. The main objective of this work was to study such conformity based on genetic stability at simple sequence repeat (SSR) loci during somatic embryogenesis in maritime pine (Pinus pinaster Ait.). Embryogenic cell lines (ECLs) subjected to tissue proliferation during 6, 14 or 22 months, as well as emblings regenerated from several ECLs, were analyzed. Genetic variation at seven SSR loci was detected in ECLs under proliferation conditions for all time points, and in 5 out of 52 emblings recovered from somatic embryos. Three of these five emblings showed an abnormal phenotype consisting mainly of plagiotropism and loss of apical dominance. Despite the variation found in somatic embryogenesis-derived plant material, no correlation was established between genetic stability at the analyzed loci and abnormal embling phenotype, present in 64% of the emblings. The use of microsatellites in this work was efficient for monitoring mutation events during the somatic embryogenesis in P. pinaster. These molecular markers should be useful in the implementation of new breeding and deployment strategies for improved trees using SE.

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Section: Introductionmentioning

confidence: 99%

Analysis of genetic stability at SSR loci during somatic embryogenesis in maritime pine (Pinus pinaster)

et al. 2009

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“…Conservation policies will depend to a large extent on our knowledge of historic and genetic relationship among these species. AFLP has proved to be a powerful marker technology to assess diversity and genetic differentiation among plant populations, to help establish reliable taxonomic relationships between species or different genotypes of the same species (MARIETTE et al, 2002;VAN DER MERWE et al, 2000;MACE et al, 1999;ZHANG et al, 1999;SINGH et al, 1999;PAUL et al, 1997), check genetic stability in somatic embryos (HORNERO et al, 2001a) or identify molecular markers linked to disease resistance and economically important traits (CERVERA et al, 1996;THOMAS et al, 1995). In Q. suber, quality of cork is economically the most relevant trait.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of Two Evergreen Oaks [Quercus suber (L.) and Quercus ilex subsp. rotundifolia (Lam.)] in Portugal using AFLP Markers

Coelho

Lima²,

Neves

et al. 2006

Silvae Genetica

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The genetic variability of cork oak (Quercus suber, L.) in Portugal was evaluated by AFLP using five primer combinations. Three hundred and thirteen trees from three geographically contrasting regions exhibited a high level of genetic variation. The genetic profile of each individual is composed of 291 loci, randomly positioned in the genome and consists of monomorphic and polymorphic fragments. Similarities and dissimilarities among the individuals were quantitatively evaluated by numerical taxonomy. The overall sample shows a proportion of AFLP polymorphic markers of 71%, denoting a high level of variability. Ninety percent of the polymorphic markers identified in cork oak genotypes are uniformly distributed throughout the cork oak populations of Algarve, Alentejo and Trás-os-Montes regions. The coefficients of genetic similarity vary from 0.61 to 0.88 implying that 60% of fragments found are common. A sample of 52 holm oak [Quercus ilex subsp. rotundifolia (Lam.)] trees from overlapping areas was also analysed by AFLP with the same five primer combinations. However the codification of markers together with those selected on cork oak profiles was feasible with only one primer combination due to an apparent much higher polymorphism. AFLP and numerical taxonomy analysis enabled to differentiate the taxa and showed that the level of similarity observed between the profiles of the individuals from holm oak species was lower than that observed in cork oak, implying that apparently the degree of polymorphism is higher in Q. ilex subsp. rotundifolia than that quantified in Q. suber. A Bayesian approach was used to assess Q. suber total genetic diversity (Ht = 0.2534, P < 0.001) of which 1.7% (Fst = 0.0172, P < 0.001) was assigned to differences among populations. Analysis of molecular variance (AMOVA) showed that most genetic variation is comprised within populations (96%) while 3.6% is among populations (Φst = 0.036, P < 0.001). Differences among populations within geographic regions account for 2.6% (Φsc = 0.026, P < 0.001) of the total variation and only 1.3% (Φct = 0.013, P = 0.007) is attributed to variation among regions denoting little differentiation of populations over a range of 700 km.

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“…Microsatellites and AFLP are among the most used markers in Quercus. AFLP markers detected changes in cork oak embryogenic lines (Hornero et al, 2001). We also used AFLP to test putative genetic instability during the developed cryopreservation/somatic embryogenesis processes of cork oak (Fernandes et al, 2008).…”

Section: Tissuementioning

confidence: 99%

Somatic Embryogenesis and Cryopreservation in Forest Species: The Cork Oak Case Study

Santos¹

2012

Current Frontiers in Cryopreservation

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Early Checking of Genetic Stability of Cork Oak Somatic Embryos by AFLP Analysis

Cited by 33 publications

References 31 publications

Analysis of genetic stability at SSR loci during somatic embryogenesis in maritime pine (Pinus pinaster)

Analysis of genetic stability at SSR loci during somatic embryogenesis in maritime pine (Pinus pinaster)

Genetic Diversity of Two Evergreen Oaks [Quercus suber (L.) and Quercus ilex subsp. rotundifolia (Lam.)] in Portugal using AFLP Markers

Somatic Embryogenesis and Cryopreservation in Forest Species: The Cork Oak Case Study

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