Early changes in the urine proteome in a diethyldithiocarbamate-induced chronic pancreatitis rat model

Zhang, Linpei; Li, Yuqiu; Gao, Youhe

doi:10.1016/j.jprot.2018.07.015

Cited by 32 publications

(25 citation statements)

References 35 publications

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“…However, urine has been shown to provide clues for the early diagnosis of disease in a variety of animal models in our previous work. [34][35][36][37][38][39][40][41] The W256 breast carcinoma cell line has been used to construct a rat model of brain metastases that has a histopathology similar to that of patients with brain metastases. [42][43][44][45] Direct intracerebral injection of tumor cells can lead to tumor cell growth in the brain, which is the most well-controlled and reproducible method for establishing animal models of brain metastases.…”

Section: Discussionmentioning

confidence: 99%

Dynamic urinary proteomic analysis in a Walker 256 intracerebral tumor model

Zhang

Meng

et al. 2019

Cancer Medicine

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Background Patients with primary and metastatic brain cancer have an extremely poor prognosis, mostly due to the late diagnosis of disease. Urine, which lacks homeostatic mechanisms, is an ideal biomarker source that accumulates early and highly sensitive changes to provide information about the early stage of disease. Methods A rat model mimicking the local tumor growth process in the brain was established with intracerebral Walker 256 (W256) cell injection. Urine samples were collected on days 3, 5, and 8 after injection, and then analyzed by liquid chromatography coupled with tandem mass spectrometry. Results In the intracerebral W256 model, no obvious clinical manifestations or abnormal magnetic resonance imaging (MRI) signals were found on days 3 or 5; at these time points, 9 proteins were changed significantly in the urine of all eight tumor rats. On day 8, when tumors were detected by MRI, 25 differential proteins were identified, including 10 that have been reported to be closely related to brain metastasis or primary tumors. The differential urinary proteome was compared with those from the subcutaneous W256 model and the intracerebral C6 model. Few differential proteins overlapped, and specific differential protein patterns were observed among the three models. Conclusions These findings demonstrate that early changes in the urine proteome can be detected in the intracerebral W256 model. The urinary proteome can reflect the difference when tumor cells with different growth characteristics are inoculated into the brain and when identical tumor cells are inoculated into different areas, specifically, the subcutis and the brain.

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Section: Discussionmentioning

confidence: 99%

Dynamic urinary proteomic analysis in a Walker 256 intracerebral tumor model

Zhang

Meng

et al. 2019

Cancer Medicine

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“…In the model of focal segmental glomerulosclerosis induced by doxorubicin, the urinary proteins were continuously changing with disease progression [7]. There are other models of organ diseases as follows: bleomycin-induced pulmonary fibrosis model [8], experimental autoimmune myocarditis model [9], rat model of bacterial meningitis [10], glioblastoma animal model [11], tumor model of the subcutaneous implantation of Walker 256 cells [12], rat model of chronic pancreatitis induced by diethyldithiocarbamate [13], rat model of NuTu-19 lung metastasis [14], Walker 256 intracerebral tumor model [15], and Walker 256 lung metastasis rat model [16]. The urinary proteome changes significantly before the clinical signs and histopathological changes have occurred.…”

Section: Introductionmentioning

confidence: 99%

Early urine proteome changes in an implanted bone cancer rat model

Wang

Qin

et al. 2019

Preprint

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In this study, Walker 256 cells were implanted into rat tibiae. Urine samples were then collected on days 3, 5, 7, and 13 and were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). With label-free quantification, 25 proteins were found to change significantly in the urine of the tumor group mice compared with the proteins in the urine of the control group mice; this was even the case when there were no significant lesions identified in the Computed Tomography(CT) examination. Among these differentially proteins, 7 were reported to be associated with tumor bone metastasis. GO analysis shows that the differential proteins on day 3 were involved in several responses, including the acute phase response, the adaptive immune response and the innate immune response. The differentially proteins on day 7 were involved in the mineral absorption pathway. The differentially proteins on day 13 were involved in vitamin D binding and calcium ion binding. These processes may be associated with bone metastasis. Our results demonstrate that urine could sensitively reflect the changes in the early stage of implanted bone cancer; this provides valuable clues for future studies of urine biomarkers for tumor bone metastasis.

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“…Changes caused by chronic pancreatitis can be reflected in early urinary proteins. New clues for the early diagnosis of chronic pancreatitis have even been found with a small number of animal models [7]. In the urine of bacterial meningitis rat models, differential urinary proteins have also been detected, many of which have been reported as biomarkers of bacterial meningitis in the cerebrospinal fluid and blood [8].…”

Section: Introductionmentioning

confidence: 99%

A device to collect exhaled breath to study biomarkers in small animal models

Hou

et al. 2019

Preprint

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Biomarkers are measurable changes associated with disease. Exhaled breath provides many signs of bodily changes and has been proposed to be a good early biomarker source because it lacks homeostatic mechanisms. Earlier biomarker detection can provide earlier diagnosis, which can bring about more choices and more time for treatment. The benefit of studying exhaled breath in animal models is that most interfering factors can be avoided, and earlier changes in disease may be found. However, there is currently no device that can effectively collect exhaled breath from small animals. In this study, such a device was designed, constructed, and used for the study of candidate biomarkers from the exhale breaths of a papain-induced pulmonary emphysema rat model.

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Early changes in the urine proteome in a diethyldithiocarbamate-induced chronic pancreatitis rat model

Cited by 32 publications

References 35 publications

Dynamic urinary proteomic analysis in a Walker 256 intracerebral tumor model

Dynamic urinary proteomic analysis in a Walker 256 intracerebral tumor model

Early urine proteome changes in an implanted bone cancer rat model

A device to collect exhaled breath to study biomarkers in small animal models

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