Early Changes in Cement–Bone Fixation Using a Novel Rat Knee Replacement Model

Mann, Kenneth A.; Miller, Mark A.; Amendola, Richard L.; Cyndari, Karen I.; Horton, Jason A.; Damron, Timothy A.; Oest, Megan E.

doi:10.1002/jor.24390

Cited by 6 publications

(14 citation statements)

References 39 publications

(65 reference statements)

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“…As such, the highest fluid shear stresses should occur at the cement border. Indeed, with in vivo service, we find that resorption initiates at the cement border in the rat model 13 as well as in human retrievals 9 . The cement border also has direct access to marrow, which serves as a primary reservoir for bone remodeling cells.…”

Section: Discussionmentioning

confidence: 69%

“…For example, the cement volume in the rat knee replacement would be three orders of magnitude smaller than the human knee replacement, and cement volume would influence the amount of heat generated by the polymerizing cement 36 . Thermal necrosis that might occur in human implantation would not be anticipated in the rat 13 . In the rat model, the proximal tibial growth plate remains open throughout life.…”

Section: Discussionmentioning

confidence: 99%

“…Nonsurvival, bilateral tibial knee replacement surgery was performed on four rats (8 knees) at 12 weeks of age. Details of the implant design and surgical procedure are provided elsewhere 13 . Briefly, under isoflurane anesthesia, a parapatellar arthrotomy was performed, the patella was dislocated laterally, and a 1.0 mm diameter surgical burr was used to remove epiphyseal bone above the growth plate (Figure 1A).…”

Section: Methodsmentioning

confidence: 99%

“…To explore the biological and mechanobiological changes of trabecular resorption for cement‐bone interdigitated regions, a cemented rat knee replacement model was developed 13 . This preclinical model has features consistent with human joint replacement including loading from a tibial tray implant through a cement layer that is interdigitated with trabecular bone.…”

Section: Introductionmentioning

confidence: 99%

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Similitude of cement‐bone micromechanics in cemented rat and human knee replacement

Mann

Miller

Tatusko

et al. 2020

Journal Orthopaedic Research

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A preclinical rat knee replacement model was recently developed to explore the biological and mechanobiological changes of trabecular resorption for cement‐bone interdigitated regions. The goal here was to evaluate the relevance of this model compared with human knee replacement with regards to functional micromechanics. Eight nonsurvival, cemented knee replacement surgeries were performed, the interdigitated gap morphology was quantified, and interface micromotion between cement and bone was measured for 1 to 5 bodyweight loading. Computational fluid dynamics modeling of unit cell geometries with small gaps between trabeculae and cement was used to estimate fluid flow. Gap width (3.6 μm) was substantially smaller compared with cement‐bone gaps reported in human knee replacement (11.8 μm). Micromotion at the cement‐bone border was also decreased for the rat knee replacement (0.48 μm), compared with human (1.97 μm), for 1 bodyweight loading. However, the micromotion‐to‐gap width ratio (0.19 and 0.22 for, rat and human), and estimated fluid shear stress (6.47 and 7.13 Pa, for rat and human) were similar. Replicating the fluid dynamic characteristics of cement‐bone interdigitated regions in human knee replacements using preclinical models may be important to recapitulate trabecular resorption mechanisms due to proposed supraphysiologic fluid shear stress. Statement of clinical significance: local cement‐bone micromotion due to joint loading may contribute to the process of clinical loosening in total joint replacements. This work shows that while micromotion and gap morphology are diminished for the rat knee model compared to human, the motion‐to‐gap ratio, and corresponding fluid shear stress are of similar magnitudes

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Section: Discussionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Similitude of cement‐bone micromechanics in cemented rat and human knee replacement

Mann

Miller

Tatusko

et al. 2020

Journal Orthopaedic Research

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“…Niels et al [34] used alloy prosthesis on the femur side and high density polyethylene prosthesis on the tibia side, both with cementless xation to made knee replacement model, but the prosthesis was loose and displaced after the operation. Kenneth [35] used polyetheretherketones(PEEK) material to make a rat model of tibial knee replacement, which was xed with bone cement successfully. Carli [36] et al made a mouse model of tibial knee replacement with titanium prosthesis.…”

Section: Discussionmentioning

confidence: 99%

Experimental Study of the Effects of Hypoxia Simulator on Osteointegration of Titanium Prosthesis in Osteoporotic Rats

Liu

Kang

et al. 2021

Preprint

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Purpose: Poor osseointegration is the key reason for implant failure after arthroplasty, whether in osteoporotic or normal bone conditions. To date, osseointegration remains a major challenge. Recent studies have shown that deferoxamine(DFO) can accelerate osteogenesis by activation of the hypoxia signal pathway. The purpose of this study is to test the following hypothesis: after knee replacement, intra-articular injection of DFO will promote osteogenesis and osseointegration with titanium prosthesis in the bones of osteoporotic rats.Materials and Methods: 90 female sprague-dawley rats were used for the experiment. Ovariectomy and knee arthroplasty were performed. Then, the rats were randomly divided into DFO and control group(n=40 per group). The two groups were treated by intraarticular injection of DFO and saline respectively. After 2 weeks, polymerase chain reaction(PCR) and immunohistochemistry were used to evaluate the levels of HIF-1a, VEGF and CD31. After 12 weeks, the specimens were examined by micro CT, biomechanics and histopathology to evaluate osteogenesis and osseointegration.Results: The results of PCR showed mRNA levels of VEGF and CD31 in DFO group were significantly higher than those in control group. The immunohistochemistry results indicated positive cell expressions of HIF-1a, VEGF and CD31 in DFO group were also higher. Compared to control group, the microCT parameters of BMD, BV/TV, TB.N, TB.Th were significantly higher. The maximal pull-out force and the bone-to-implant contact (BIC) value were also higher . Conclusions: The local administration of DFO which is used to activate HIF-1a signaling pathway can promote osteogenesis and osseointegration with the prosthesis in osteoporotic bone.

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Progressive loss of implant fixation in a preclinical rat model of cemented knee arthroplasty

Mann

Miller

Rossow

et al. 2021

Journal Orthopaedic Research

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Aseptic loosening of total knee arthroplasty continues to be a challenging clinical problem. The progression of the loosening process, from the initial well-fixed component, is not fully understood. In this study, loss of fixation of cemented hemiarthroplasty was explored using 9-month-old Sprague-Dawley rats with 0, 2, 6, 12, 26 week end points. Morphological and cellular changes of cement-bone fixation were determined for regions directly below the tibial tray (epiphysis) and distal to the tray (metaphysis). Loss of fixation, with a progressive increase in cement-bone gap volume was found in the epiphysis (0.162 mm 3 /week), but did not progress appreciably in the metaphysis (0.007 mm 3 /week). In the epiphysis, there was an early and sustained elevation of osteoclasts adjacent to the cement border and development of a fibrous tissue layer between the cement and bone. There was early formation of bone around the cement in the metaphysis, resulting in a condensed bone layer without osteoclastic bone resorption or development of a fibrous tissue layer. Implant positioning was also an important factor in the cement-bone gap formation, with greater gap formation for implants that were placed medially on the tibial articular surface. Loss of fixation in the rat model mimicked patterns found in human arthroplasty where cement-bone gaps initiate under the tibial tray, at the periphery of the implant. This preclinical model could be used to study early biological response to cemented fixation and associated contributions of mechanical instability, component alignment, and periprosthetic inflammation.

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Early Changes in Cement–Bone Fixation Using a Novel Rat Knee Replacement Model

Cited by 6 publications

References 39 publications

Similitude of cement‐bone micromechanics in cemented rat and human knee replacement

Similitude of cement‐bone micromechanics in cemented rat and human knee replacement

Experimental Study of the Effects of Hypoxia Simulator on Osteointegration of Titanium Prosthesis in Osteoporotic Rats

Progressive loss of implant fixation in a preclinical rat model of cemented knee arthroplasty

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