Early candidate biomarkers in urine of Walker-256 lung metastasis rat model

Wei, Jing; Ni, N.; Zhang, Linpei; Gao, Youhe

doi:10.1101/306050

Cited by 6 publications

(8 citation statements)

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“…The results of the W256 liver tumour model were compared with three different previously published studies from our laboratory: (1) W256 subcutaneous model (Wu, Guo & Gao, 2017); (2) W256 lung metastasis model (Wei et al, 2018); (3) W256 intracerebral tumour model . In (1), the tumour-bearing rats were subcutaneously inoculated with 2 × 10 6 viable W256 cells in 200 µL of PBS into the right flank of the animal.…”

Section: Comparison Methods Of Different W256 Tumour Modelsmentioning

confidence: 99%

“…Protein AMBP, a liver-specific precursor, is also a precursor of the heme-binding protein that counteracts the disruption of free haemoglobin (Van den Figure 4 The overlapping differential proteins in urine samples of the four different W256 tumour models. The comparison data is from previously published studies (Wu, Guo & Gao, 2017;Wei et al, 2018;Zhang et al, 2018). The comparison data is from previously published studies (Wu, Guo & Gao, 2017;Wei et al, 2018;Zhang et al, 2018).…”

Section: Comparison Of Urinary Proteins In Different Tumour Modelsmentioning

confidence: 99%

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Early changes in the urine proteome in a rat liver tumour model

Zhang

Gao

2020

PeerJ

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Background Urine, as a potential biomarker source among body fluids, can accumulate many early changes in the body due to the lack of mechanisms to maintain a homeostatic state. This study aims to detect early changes in the urinary proteome in a rat liver tumour model. Methods The tumour model was established with the Walker-256 carcinosarcoma cell line (W256). Urinary proteins at days 3, 5, 7 and 11 were profiled by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Compared with controls, differential proteins were selected. Associations of differential proteins with cancer were retrieved. Results At days 3, 5, 7 and 11, five, fifteen, eleven and twelve differential proteins were identified, respectively. Some of the differential proteins were reported to be associated with liver cancer. This differential urinary protein pattern was different from the patterns in W256 subcutaneous, lung metastasis and intracerebral tumour models. Conclusions This study demonstrates that (1) early changes in urinary proteins can be found in the rat liver tumour model; (2) urinary proteins can be used to differentiate the same tumour cells grown in different organs.

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Section: Comparison Methods Of Different W256 Tumour Modelsmentioning

confidence: 99%

Section: Comparison Of Urinary Proteins In Different Tumour Modelsmentioning

confidence: 99%

Early changes in the urine proteome in a rat liver tumour model

Zhang

Gao

2020

PeerJ

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“…7). at early stages based on the data obtained from the studies of our laboratory published before [11,111,112] .…”

Section: Functional Analysis Of Differential Proteinsmentioning

confidence: 99%

Early changes in the urine proteome in a rat liver tumor model

Zhang

Gao

2019

Preprint

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Urine, as a potential biomarker source among the body fluids, can accumulate many changes in the body due to the lack of a mechanism to maintain a homeostatic state. Previous studies have demonstrated that proteomic technology can find many potential biomarkers to reflect different diseases in the urine.This study aims to detect early changes in the urinary proteome in a rat liver tumor model. The tumor model was established with the Walker-256 carcinosarcoma cell line (W256). Compared to before the injection, ninety-five differential proteins were significantly changed in the experimental rats. At day 3, twelve proteins were identified in the absence of pathological changes, and four of them were altered at all four time-points (B2MG, VCAM1, HA11, and LG3BP). Seven had previously been associated with liver cancer. At day 5, fifty-two differential proteins were identified. At day 7 and day 11, there was a significant decrease in the body weight of the rats, and tumor tissue was observed in the liver. Fifty-two and forty differential proteins were changed significantly at day 7 and day 11, respectively. Of the proteins that were identified at these three time-points, and twenty-four were reported to be associated with liver cancer. Comparing the differential urinary proteins and biological processes of liver tumor model with those in different models of W256 grown in other organs, specific differential protein patterns were found among the four models, which indicates that the differential urinary proteins can reflect the differences when the same tumor cell grown in different organs.Significance: This study demonstrated that (1) the rat liver tumor model caused early changes in urinary proteins may give new insight into the early diagnosis of liver cancer; (2) the same tumor cell grown in different organs can be reflected in differential urinary proteins.All rats were housed under a standard 12-h light/12-h dark cycle, and the room temperature and humidity were maintained at a standard level (22 ± 1 °C, 65-70%). Tumor cell line and CultureWalker 256 tumor cells were cultured in the ascitic fluid of Wistar rats. All cells were harvested from the rats who were given an intraperitoneal injection of 107 Walker 256 carcinoma cells after two cycles of 7 d cell passage. Then, W256 cells were resuspended in phosphate-buffered saline (PBS) before injection. The viability of the cells was detected by the Trypan blue exclusion test using a Neubauer chamber. Rat models of liver cancerA tumor-bearing animal model was established in this study. All Wistar rats were randomly divided into different groups: the control group (n =5), and the Walker-256 (W256) tumor-bearing group (n =12).After anesthesia, the left medial lobe of the liver was exposed. W256 cells (2.5× 10 5 ) were visually injected under the hepatic capsule into this lobe. The injection volume is 0.1 ml. An equal volume of PBS was also injected into the same location of the control rats. Histological analysisIn the W256 model, the livers of the experimental and cont...

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“…In the model of focal segmental glomerulosclerosis induced by doxorubicin, the urinary proteins were continuously changing with disease progression [7]. There are other models of organ diseases as follows: bleomycin-induced pulmonary fibrosis model [8], experimental autoimmune myocarditis model [9], rat model of bacterial meningitis [10], glioblastoma animal model [11], tumor model of the subcutaneous implantation of Walker 256 cells [12], rat model of chronic pancreatitis induced by diethyldithiocarbamate [13], rat model of NuTu-19 lung metastasis [14], Walker 256 intracerebral tumor model [15], and Walker 256 lung metastasis rat model [16]. The urinary proteome changes significantly before the clinical signs and histopathological changes have occurred.…”

Section: Introductionmentioning

confidence: 99%

Early urine proteome changes in an implanted bone cancer rat model

Wang

Qin

et al. 2019

Preprint

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In this study, Walker 256 cells were implanted into rat tibiae. Urine samples were then collected on days 3, 5, 7, and 13 and were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). With label-free quantification, 25 proteins were found to change significantly in the urine of the tumor group mice compared with the proteins in the urine of the control group mice; this was even the case when there were no significant lesions identified in the Computed Tomography(CT) examination. Among these differentially proteins, 7 were reported to be associated with tumor bone metastasis. GO analysis shows that the differential proteins on day 3 were involved in several responses, including the acute phase response, the adaptive immune response and the innate immune response. The differentially proteins on day 7 were involved in the mineral absorption pathway. The differentially proteins on day 13 were involved in vitamin D binding and calcium ion binding. These processes may be associated with bone metastasis. Our results demonstrate that urine could sensitively reflect the changes in the early stage of implanted bone cancer; this provides valuable clues for future studies of urine biomarkers for tumor bone metastasis.

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Early candidate biomarkers in urine of Walker-256 lung metastasis rat model

Cited by 6 publications

References 212 publications

Early changes in the urine proteome in a rat liver tumour model

Early changes in the urine proteome in a rat liver tumour model

Early changes in the urine proteome in a rat liver tumor model

Early urine proteome changes in an implanted bone cancer rat model

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