Early blastocyst expansion in euploid and aneuploid human embryos: evidence for a non-invasive and quantitative marker for embryo selection

Huang, Tian-Sen; Huang, David H; Ahn, Hyeong Jun; Arnett, C.M.; Huang, Christopher T.F.

doi:10.1016/j.rbmo.2019.01.010

Cited by 29 publications

(19 citation statements)

References 65 publications

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“…Our group and others have previously shown that blastocyst euploidy rates differ by age, morphology, and expansion rate (Huang et al, 2019;McDaniel et al, 2020;Minasi et al, 2016). At our center the euploidy rate is 76% at age 26 and decreases to 24% at age 43.…”

Section: Introductionsupporting

confidence: 43%

Effect of age and morphology on sustained implantation rate after euploid blastocyst transfer

Awadalla

Vestal

McGinnis

et al. 2021

Reproductive BioMedicine Online

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Section: Introductionsupporting

confidence: 43%

Effect of age and morphology on sustained implantation rate after euploid blastocyst transfer

Awadalla

Vestal

McGinnis

et al. 2021

Reproductive BioMedicine Online

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“…To our knowledge, this is the first time that the trophectoderm cell cycle length is measured, although the rapid expansion has been associated with an integrative cellular mitosis in trophectoderm cells (64). To ensure that the ccTroph length was representative of each embryo, we performed the measurement on two different cells for 100 embryos without obtaining significant variations (unpublished data).…”

Section: Discussionmentioning

confidence: 99%

Novel and conventional embryo parameters as input data for artificial neural networks: an artificial intelligence model applied for prediction of the implantation potential

Bori

Payá

Alegre

et al. 2020

Fertility and Sterility

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“…The observed smaller size and lower expansion rate for embryos that did not result in an ongoing pregnancy might have a genetic cause. A previous study observed in a small cohort of embryos undergoing preimplantation genetic testing for aneuploidy (PGT-A) that euploid blastocysts expanded significantly faster than aneuploid blastocysts [ 25 ]. Another study compared mitotic spindles in slow and fast developing human blastocysts.…”

Section: Discussionmentioning

confidence: 99%

Longitudinal surface measurements of human blastocysts show that the dynamics of blastocoel expansion are associated with fertilization method and ongoing pregnancy

Marion

Chavli

Laven

et al. 2022

Reprod Biol Endocrinol

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Background Despite all research efforts during this era of novel time-lapse morphokinetic parameters, a morphological grading system is still routinely being used for embryo selection at the blastocyst stage. The blastocyst expansion grade, as evaluated during morphological assessment, is associated with clinical pregnancy. However, this assessment is performed without taking the dynamics of blastocoel expansion into account. Here, we studied the dynamics of blastocoel expansion by comparing longitudinal blastocoel surface measurements using time-lapse embryo culture. Our aim was to first assess if this is impacted by fertilization method and second, to study if an association exists between these measurement and ongoing pregnancy. Methods This was a retrospective cohort study including 225 couples undergoing 225 cycles of in vitro fertilization (IVF) treatment with time-lapse embryo culture. The fertilization method was either conventional IVF, intracytoplasmic sperm injection (ICSI) with ejaculated sperm or ICSI with sperm derived from testicular sperm extraction (TESE-ICSI). This resulted in 289 IVF embryos, 218 ICSI embryos and 259 TESE-ICSI embryos that reached at least the full blastocyst stage. Blastocoel surface measurements were performed on time-lapse images every hour, starting from full blastocyst formation (tB). Linear mixed model analysis was performed to study the association between blastocoel expansion, the calculated expansion rate (µm2/hour) and both fertilization method and ongoing pregnancy. Results The blastocoel of both ICSI embryos and TESE-ICSI embryos was significantly smaller than the blastocoel of IVF embryos (beta -1121.6 µm2; 95% CI: -1606.1 to -637.1, beta -646.8 µm2; 95% CI: -1118.7 to 174.8, respectively). Still, the blastocoel of transferred embryos resulting in an ongoing pregnancy was significantly larger (beta 795.4 µm2; 95% CI: 15.4 to 1575.4) and expanded significantly faster (beta 100.9 µm2/hour; 95% CI: 5.7 to 196.2) than the blastocoel of transferred embryos that did not, regardless of the fertilization method. Conclusion Longitudinal blastocyst surface measurements and expansion rates are promising non-invasive quantitative markers that can aid embryo selection for transfer and cryopreservation. Trial registration Our study is a retrospective observational study, therefore trial registration is not applicable.

show abstract

Early blastocyst expansion in euploid and aneuploid human embryos: evidence for a non-invasive and quantitative marker for embryo selection

Cited by 29 publications

References 65 publications

Effect of age and morphology on sustained implantation rate after euploid blastocyst transfer

Effect of age and morphology on sustained implantation rate after euploid blastocyst transfer

Novel and conventional embryo parameters as input data for artificial neural networks: an artificial intelligence model applied for prediction of the implantation potential

Longitudinal surface measurements of human blastocysts show that the dynamics of blastocoel expansion are associated with fertilization method and ongoing pregnancy

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