Early bacterial dependent induction of inducible nitric oxide synthase (iNOS) in epithelial cells upon transfer of CD45RBhigh CD4+ T cells in a model for experimental colitis

Dijkstra, Gerard; Yuvaraj, Saravanan; Jiang, Han-Qing; Bun, Judy C. A. M.; Moshage, Han; Kushnir, Natasha; Peppelenbosch, Maikel P.; Cebra, John J.; Bos, Nicolaas

doi:10.1002/ibd.20262

Cited by 18 publications

(15 citation statements)

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“…The NFκB mediated inducible nitric oxide synthase was equally increased in the inflamed ileum of both ATG16L1 genotypes36 (see online supplementary figure S3A). Except for 1 patient homozygous for the ATG16L1 protective allele, the defensin 5 and 6 gene expression (Paneth cell function) decreased upon inflammation in all other patients (p<0.05 for risk allele group) (figure 4A,B).…”

Section: Resultsmentioning

confidence: 93%

TheATG16L1–T300Aallele impairs clearance of pathosymbionts in the inflamed ileal mucosa of Crohn's disease patients

Sadabad

Regeling

Goffau

et al. 2014

Gut

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Section: Resultsmentioning

confidence: 93%

TheATG16L1–T300Aallele impairs clearance of pathosymbionts in the inflamed ileal mucosa of Crohn's disease patients

Sadabad

Regeling

Goffau

et al. 2014

Gut

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“…Recently, Kretzmann et al [43] showed that inhibition of iNOS may contribute to the therapeutic effect of glutamine in rats with 2,4,6-trinitrobenzene sulfonic acid-induced colitis. Dijkstra et al [44] also reported that the expression of epithelial iNOS is highly bacterium-specific and can correlate with the severity of the disease, suggesting an important role for this enzyme in the development of IBD. Therefore, the anti-colitis activity of W. chinesis may be mediated in part via its effect on inhibition of iNOS activation in colon tissues.…”

Section: Discussionmentioning

confidence: 98%

Dietary Uptake of Wedelia chinensis Extract Attenuates Dextran Sulfate Sodium-Induced Colitis in Mice

Huang

Wen²,

Chen

et al. 2013

PLoS ONE

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ScopeTraditional medicinal herbs are increasingly used as alternative therapies in patients with inflammatory diseases. Here we evaluated the effect of Wedelia chinensis, a medicinal herb commonly used in Asia, on the prevention of dextran sulfate sodium (DSS)-induced acute colitis in mice. General safety and the effect of different extraction methods on the bioactivity of W. chinensis were also explored.Methods and ResultsC57BL/6 mice were administrated hot water extract of fresh W. chinensis (WCHF) orally for one week followed by drinking water containing 2% DSS for nine days. WCHF significantly attenuated the symptoms of colitis including diarrhea, rectal bleeding and loss of body weight; it also reduced the shortening of colon length and histopathological damage caused by colonic inflammation. Among four W. chinensis extracts prepared using different extraction techniques, WCHF showed the highest anti-colitis efficacy. Analyses of specific T-cell regulatory cytokines (TNF-α, IL-4, IFN-γ, IL-17, TGF-β, IL-12) revealed that WCHF treatment can suppress the Th1 and Th17, but not Th2, responses in colon tissues and dendritic cells of DSS-induced colitis mice. A 28-day subacute toxicity study showed that daily oral administration of WCHF (100, 500, 1000 mg/kg body weight) was not toxic to mice.ConclusionTogether, our findings suggest that specific extracts of W. chinensis have nutritional potential for future development into nutraceuticals or dietary supplements for treatment of inflammatory bowel disease.

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“…Changes in the microbial content of the gut also lead to an imbalance between the beneficial metabolic byproducts and bacterial toxic compounds [14]. Furthermore, both culture-dependent methods and molecular approaches have shown that dysbiosis of the gut bacterial community leads to overexpression of antigenic bacterial surface proteins, which results in an exaggerated immune response and chronic immune-mediated inflammatory mucosal damage in IBD patients [15–17]. In stools from patients with colorectal carcinoma, an increase in the proportion of bacteria such as Bacteroides spp.…”

Section: Introductionmentioning

confidence: 99%

Gut microbial diversity in health and disease: experience of healthy Indian subjects, and colon carcinoma and inflammatory bowel disease patients

Bamola

Ghosh

Kapardar

et al. 2017

Microbial Ecology in Health and Disease

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Background: The intestinal microbiota, through complex interactions with the gut mucosa, play a key role in the pathogenesis of colon carcinoma and inflammatory bowel disease (IBD). The disease condition and dietary habits both influence gut microbial diversity. Objective: The aim of this study was to assess the gut microbial profile of healthy subjects and patients with colon carcinoma and IBD. Healthy subjects included ‘Indian vegetarians/lactovegetarians’, who eat plant produce, milk and milk products, and ‘Indian non-vegetarians’, who eat plant produce, milk and milk products, certain meats and fish, and the eggs of certain birds and fish. ‘Indian vegetarians’ are different from ‘vegans’, who do not eat any foods derived wholly or partly from animals, including milk products. Design: Stool samples were collected from healthy Indian vegetarians/lactovegetarians and non-vegetarians, and colon cancer and IBD patients. Clonal libraries of 16S ribosomal DNA (rDNA) of bacteria were created from each sample. Clones were sequenced from one representative sample of each group. Approximately 500 white colonies were picked at random from each sample and 100 colonies were sequenced after amplified rDNA restriction analysis. Results: The dominant phylum from the healthy vegetarian was Firmicutes (34%), followed by Bacteroidetes (15%). The balance was reversed in the healthy non-vegetarian (Bacteroidetes 84%, Firmicutes 4%; ratio 21:1). The colon cancer and IBD patients had higher percentages of Bacteroidetes (55% in both) than Firmicutes (26% and 12%, respectively) but lower Bacteroidetes:Firmicutes ratios (3.8:1 and 2.4:1, respectively) than the healthy non-vegetarian. Bacterial phyla of Verrucomicrobiota and Actinobacteria were detected in 23% and 5% of IBD and colon patients, respectively. Conclusions: Ribosomal Database Project profiling of gut flora in this study population showed remarkable differences, with unique diversity attributed to different diets and disease conditions.

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Early bacterial dependent induction of inducible nitric oxide synthase (iNOS) in epithelial cells upon transfer of CD45RBhigh CD4+ T cells in a model for experimental colitis

Abstract: These findings demonstrate that the expression of epithelial iNOS is highly bacterium-specific and correlates with the severity of disease, suggesting an important role for this enzyme in the development of IBD.

Cited by 18 publications

References 40 publications

TheATG16L1–T300Aallele impairs clearance of pathosymbionts in the inflamed ileal mucosa of Crohn's disease patients

TheATG16L1–T300Aallele impairs clearance of pathosymbionts in the inflamed ileal mucosa of Crohn's disease patients

Dietary Uptake of Wedelia chinensis Extract Attenuates Dextran Sulfate Sodium-Induced Colitis in Mice

Gut microbial diversity in health and disease: experience of healthy Indian subjects, and colon carcinoma and inflammatory bowel disease patients

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