Early and specific diagnosis of seropositivity to HIVs by an enzyme-linked immunosorbent assay using env-derived synthetic peptides

Fenouillet, Emmanuel; Am, Sorensen; Lacroix, Martial; Coutellier, A; Herson, S.; Fretz-Foucault, C; Jc, Gluckman

doi:10.1097/00002030-199011000-00013

Cited by 6 publications

(5 citation statements)

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“…Average A(n = 32) B (n = 30) C(n = 31) A peptide corresponding to gf)4I immunodominant epitope [16] (gp41 IDE; aa 590-620; described in [17]) was purchased from IAF Biochem (Laval, Quebec, Canada).…”

Section: According To the Ciinica] Categorymentioning

confidence: 99%

“…After saturation for 1 h at 20 C with PBS, 0 3% Twecn-20. and washing with wash buffer provided with the Detect HIV Kit (IAF Biochem) [17], 100/J of scrum (diluted as indicated below in the sample diluent ofthe kit) were added for 90 min at 20 C. After three washes, peroxidase-coupled mouse anti-human IgG antibodies (IAF Biochem) (diluted 1:40 in the diluent of the kit! were added for I h. After four washes, tetramethylbenzidine (IAF Biochem) (diluted 1:5 in the buffer of the kit) was added for 15 min at 20X, and the reaction was stopped with H2SO4.…”

Section: Quantification Of Anti-v3 Antibodiesmentioning

confidence: 99%

“…Peptides from the Agence Nationale pour la Recherche sur le SIDA (Paris, France) were synthesized by Neosystem SA (Strasbourg, France) according to the Los Alamos database sequences of HIV-1 strains MN. NY5, WMJ2, SF2, SC, RF and CDC4 (Table I) A peptide corresponding to gf)4I immunodominant epitope [16] (gp41 IDE; aa 590-620; described in [17]) was purchased from IAF Biochem (Laval, Quebec, Canada).…”

Section: Peptides and Proteinmentioning

confidence: 99%

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Anti-V3 antibody reactivity correlates with clinical stage of HIV-1 infection and with serum neutralizing activity

Fenouillet

Blanes

Benjouad

et al. 1995

Clinical and Experimental Immunology

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SUMMARY During HIV‐1 infection, the current tenet is that only anti‐gag antibodies decline, while those directed at env remain stable. Among the latter antibodies, those directed to the V3 domain of gpl20 are assumed to play a role in the immune surveillance against HIV‐1. We investigated the correlation between anti‐V3 antibody levels and the clinical stage of infection and the ability to neutralize syncytium formation. Using a V3‐specific antigen‐limited ELISA, we analysed the antibody levels of a panel of 93 HIV‐1+ sera to V3 peptides derived from different HIV‐1 strains and from the North American/European consensus sequence V3(Cs). Sera preferentially recognized V3 peptides from the representative V3(MN) strain and V3(Cs). Antibody reactivity to V3(MN) or V3(Cs) actually declined in relation with progression to AIDS, while antibodies against whole recombinant gpt60 or gp41 immunodominant epitope remained stable. There was a strong correlation (P < 00001) between anti‐V3 (Cs)/V3(MN) antibody levels and serum titres that neutralized HIV‐1MN‐mediated syncytia. Serology based on V3‐specific antigen‐limited ELISA indicates that anti‐V3 antibody reactivity may decline during the course of HIV infection.

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Section: According To the Ciinica] Categorymentioning

confidence: 99%

Section: Quantification Of Anti-v3 Antibodiesmentioning

confidence: 99%

Section: Peptides and Proteinmentioning

confidence: 99%

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Anti-V3 antibody reactivity correlates with clinical stage of HIV-1 infection and with serum neutralizing activity

Fenouillet

Blanes

Benjouad

et al. 1995

Clinical and Experimental Immunology

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“…Ob Peptid-ELISA den Western Blot und den RIPA als Bestätigungstest ablösen werden, läßt sich zur Zeit nicht mit Sicherheit sagen. Einige Untersuchungen deuten darauf hin, daß mit diesen Verfahren eine hohe Sensitivität, Spezifität und ein hoher prädiktiver Wert erreicht werden können (15). Bisher fehlen aber Studien an großen Kollektiven, in denen verschiedene Peptid-ELISAs mit anderen ELISA-Verfahren und dem Western Blot oder RIPA verglichen worden sind.…”

Section: Antikörperbestätigungstestsunclassified

“…Da jedes infektiöse Partikel aber ein Molekül Nukleinsäure enthält, erschien es den Forschern möglich, die Nukleinsäure des Virus zu isolieren und durch molekularbiologische Verfahren zu verstärken. Dies gelang den Wissenschaftlern von Chiron Corporation im Jahre 1987 (2,15). Die aus Serum extrahierte Nukleinsäure wurde am CDC (Center for Disease Control, Atlanta/USA) ausführlich charakterisiert, sie enthielt mehr als 10 6 Schimpansen-fnfektionsdosen (chimp infectious dose, CID) pro ml.…”

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1991

LaboratoriumsMedizin

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A viräl agent that is neither hepatitis Ävirus nor hepatitis B virus has been recognized äs the cause of most cases of transfusion-associated hepatitis, and for years the disease has been referred to äs non A, non B hepatitis (NANBH). This agent acccounts for one-third of all cases of acute hepatitis in industrialized nations, and the majority ofinfections progress to a chronic state (1). Detection ofthe specific virus and its antibodies eluded scientific efforts until 1987, when a small piece of nucleic acid from the virus was isolated by molecular cloning (2). This clone proved to contain the genetic code for a viral protein, and the protein could be used in an immunoassay to detect antibodies in the'sera of infected patients (3). The agent which contained this nucleic acid was named hepatitis C (HCV), and subsequent studies have proven that HCV causes most cases of NANBH in Europe, America, and Japan (1). Commercial tests for anti HCV have now been developed and are being used diagnostically or for screening donor blood. In order to better Interpret the clinical significance of a positive fest result or the efficiency of the screening test, it is useful to review its performance characteristics. This review examines data particlarly äs they apply to detectability and specificity. Second generation antibody tests have recently been developed, and the performance of the Abbott Laboratories second generation is presented and compared to the first generation methods.

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Immune Response to Retroviral Infection

Norley

Kurth

1994

The Retroviridae

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Early and specific diagnosis of seropositivity to HIVs by an enzyme-linked immunosorbent assay using env-derived synthetic peptides

Cited by 6 publications

References 0 publications

Anti-V3 antibody reactivity correlates with clinical stage of HIV-1 infection and with serum neutralizing activity

Anti-V3 antibody reactivity correlates with clinical stage of HIV-1 infection and with serum neutralizing activity

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Immune Response to Retroviral Infection

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