Earlier detection of sepsis by Candida parapsilosis using three-dimensional cytographic anomalies on the Mindray BC-6800 hematological analyzer

Gioia, Antonio La; Bombara, M; Fiorini, Fabiana; Dell'Amico, MariaChiara; Devito, Alessandra; Isola, Patrizia; Marelli, P; Fiorini, Marcello

doi:10.1515/cclm-2015-1120

Cited by 11 publications

(9 citation statements)

References 5 publications

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“…In this way, we could observe that microscopic features observed in the “in vitro” Candidemia are not different from those reported in case of in ‘in vivo’ BSI regardless of the type of Candida. 4 5 8 17 18 Even the correspondence from CBC and LDC on simulated candidemia as well as on clinical BSI has already been described 6 7 13 14. In addition, in our experimental conditions C. albicans has grown in the BACTEC system just as the clinical materials.…”

Section: Discussionmentioning

confidence: 79%

“…In addition, pseudo-erythroblastosis has been described 8 14. These changes were obtained even by adding to PB samples various amount of different Candida types to simulate, in vitro, the haematological7 13 or microbiological11 features of candidemia.…”

Section: Discussionmentioning

confidence: 99%

“…For this purpose, various amounts of C. albicans were added to PB samples to simulate BSI, which in vivo are characterised by the presence of both extracellular and phagocytised yeasts 1 2 8. Each blood sample was analysed in the BC-6800 in different times.…”

Section: Methodsmentioning

confidence: 99%

“…We have recently reported that the presence of Candida parapsilosis circulating in PB as well as the neutrophils (NE) containing phagocytised yeast blastospores cause morphological changes in cytograms of BC-6800 and that these cytographic features can be helpful in an earlier diagnosis of sepsis caused by this yeast 8. Since Candida albicans is the most prevalent species that can cause yeast bloodstream infections (BSIs),9 10 we evaluated if the cellular counting and cytographic anomalies of the BC-6800 Analyzer could be even useful in these cases just like as observed in cases of C. parapsilosis sepsis.…”

Section: Introductionmentioning

confidence: 99%

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Cytographic changes on BC-6800 Haematological Analyzer related to the presence ofCandida albicansin peripheral blood. A new tool to suspect candidemia?

et al. 2016

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Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cytographic changes on BC-6800 Haematological Analyzer related to the presence ofCandida albicansin peripheral blood. A new tool to suspect candidemia?

et al. 2016

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“…The BC-6800 haematology analyzer uses SF-Cube technology to achieve three-dimensional cell analysis for WBC, reticulocyte (RET) and NRBC with clinically relevant suspect flags [19, 20]. It provides a dedicated flag named ‘infected RBC?’ and parameters ‘InR#/InR‰’ to represent the possible presence of Plasmodium parasites and the causative agent of malaria infection.…”

Section: Discussionmentioning

confidence: 99%

Infected RBC flag/parameter provided by Mindray BC-6800 haematology analyzer aid the diagnosis of malaria

Sun

Xiang

Chen

et al. 2019

Malar J

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Background The Mindray BC-6800 haematology analyzer (BC-6800) provides a dedicated flag ‘Infected RBC’ (InR) and the number of InR (InR#)/the permillage of InR (InR‰) in routine blood testing as a screening tool for malaria in endemic areas. This study sought to evaluate the effectiveness of the BC-6800 flag parameter for aiding the diagnosis of malaria. Methods A total of 181 samples were tested using the Mindray BC-6800 haematology analyzer, including 117 malaria-infected samples collected from Yunnan, China, and 64 samples from healthy controls. Microscopy examination was conducted as reference when stained thick blood film revealed the presence of malaria parasites identified as Plasmodium vivax and Plasmodium falciparum . The receiver operating characteristic (ROC) curve analysis was developed using Analyse-it v4.92.3. The Kappa value was determined to evaluate the agreement between BC-6800 and light microscopy. Results The sensitivity of InR‰ generated by BC-6800 for P. vivax and P. falciparum was 88.3 and 24.1%, respectively; specificity of InR‰ for malaria parasites was 84.3 and 84.3%, respectively; positive predictive value and negative predictive value was 89.4 and 82.7% for P. vivax, and 52.8 and 60.3% for P. falciparum . There was a strong correlation between ΔWBC and InR‰ (R 2 = 0.9731 for P. vivax and R 2 = 0.9757 for P. falciparum ). There was also a significant correlation between parasitaemia and InR# in P. vivax -infected samples (R 2 = 0.734). InR# was evaluated using ROC curve analysis, the area under the ROC curve is 0.95 with a 95% confidence interval of 0.926 to 0.974, and the cut-off value is 0.01 × 10 9 /L for P. vivax . However, the ring stage and the early trophozoite stage of Plasmodium cannot be detected easily on BC-6800, possibly because of the small size and low nucleic acid content of these stages. Conclusions The findings suggest that the flag ‘InR’ and the parameters ‘InR#/InR‰’ provided by the BC-6800 haematology analyzer could be used to screen for malaria in a clinical setting.

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Detecting erroneous blood counts

2021

Blood Cells

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Earlier detection of sepsis by Candida parapsilosis using three-dimensional cytographic anomalies on the Mindray BC-6800 hematological analyzer

Cited by 11 publications

References 5 publications

Cytographic changes on BC-6800 Haematological Analyzer related to the presence ofCandida albicansin peripheral blood. A new tool to suspect candidemia?

Cytographic changes on BC-6800 Haematological Analyzer related to the presence ofCandida albicansin peripheral blood. A new tool to suspect candidemia?

Infected RBC flag/parameter provided by Mindray BC-6800 haematology analyzer aid the diagnosis of malaria

Detecting erroneous blood counts

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