Eag1 potassium channel immunohistochemistry in the CNS of adult rat and selected regions of human brain

Martin, Sabine; Oliveira, Cilene Lino de; Queiroz, Fernanda Mello de; Pardo, Luis A.; Stühmer, Walter; Bel, Elaine Del

doi:10.1016/j.neuroscience.2008.05.019

Cited by 59 publications

(84 citation statements)

References 35 publications

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“…Antibodies used for diagnostic purposes must detect the corresponding protein only in the affected tissues and should not give a signal in cells not expressing the protein of interest. According to previous reports of Eag1 expression (15,25), Eag1 protein was detected in human hippocampus and cells from a cervical cancer primary culture (Fig. 1A and B, respectively).…”

Section: Resultssupporting

confidence: 77%

Eag1 potassium channels as markers of cervical dysplasia

et al. 2011

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Abstract.Human ether à-go-go 1 (Eag1) potassium channels are potential tumor markers and therapeutic targets for several types of malignancies, including cervical cancer. Estrogens and human papilloma virus oncogenes regulate Eag1 gene expression, suggesting that Eag1 may already be present in pre-malignant lesions. Therefore, Eag1 could be used as an early marker and/or a potential risk indicator for cervical cancer. Consequently, we studied Eag1 protein expression by immunochemistry in cervical cancer cell lines, normal keratinocytes, cervical cytologies from intraepithelial lesions, biopsies from cervical intraepithelial neoplasias (CIN 1, 2 and 3) and in normal smears from patients taking or not taking estrogens. Two hundred and eighty-six samples obtained by liquid-based cytology and fifteen CIN biopsies were studied. We observed Eag1 protein expression in the cervical cancer cell lines, as opposed to normal keratinocytes. Eag1 was found in 67% of the cervical cytologies from low-grade intra epithelial lesions and in 92% of the samples from high-grade intraepithelial lesions, but only in 27% of the normal samples. Noteworthy, morphologically normal cells obtained from dysplastic samples also exhibited Eag1 expression. In CIN biopsies we found that the higher the grade of the lesion, the broader the Eag1 protein distribution. Almost 50% of the normal patients taking estrogens displayed Eag1 expression. We suggest Eag1 as a potential marker of cervical dysplasia and a risk indicator for developing cervical lesions in patients taking estrogens. Eag1 detection in cervical cancer screening programs should help to improve early diagnosis and decrease mortality rates from this disease.

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Section: Resultssupporting

confidence: 77%

Eag1 potassium channels as markers of cervical dysplasia

et al. 2011

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“…The ether-a-go-go (Eag1) or K v 10.1 potassium channels are widely expressed in the mammalian and rodent adult brain, 94 but their physiological function is not fully understood. The Eag1 channels are localized primarily in axons, with about 25% being present in the membrane of presynaptic-terminals.…”

Section: Potassium Channelsmentioning

confidence: 99%

Surface dynamics of voltage-gated ion channels

et al. 2016

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Neurons encode information in fast changes of the membrane potential, and thus electrical membrane properties are critically important for the integration and processing of synaptic inputs by a neuron. These electrical properties are largely determined by ion channels embedded in the membrane. The distribution of most ion channels in the membrane is not spatially uniform: they undergo activity-driven changes in the range of minutes to days. Even in the range of milliseconds, the composition and topology of ion channels are not static but engage in highly dynamic processes including stochastic or activity-dependent transient association of the pore-forming and auxiliary subunits, lateral diffusion, as well as clustering of different channels. In this review we briefly discuss the potential impact of mobile sodium, calcium and potassium ion channels and the functional significance of this for individual neurons and neuronal networks.

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“…The slides were incubated with the recombinant singlechain anti-Eag1 antibody fused to alkaline phosphatase (scFv62PhoA; optimal concentration 10 lg/ll; dilution 1:50 v/v, (Hemmerlein et al 2006). The following negative controls were performed Martin et al 2008): (a) no labeling in the brain tissue ensued when the primary antibody was replaced with normal serum; (b) preadsorption of the antibody to the corresponding fusion protein (0.5 lg/ll final concentration) containing the specific epitope used for immunization at RT for 1 h completely abolished Eag1-immunopositive reaction confirming the selectivity of scFv62-PhoA for Eag1; (c) Western Blot analysis with hEag1 antibody shows a band migrating at around 130 kDa in total brain. Tissue sections were deparaffined by rinsing twice for 20 min with xylene.…”

Section: Histological Proceduresmentioning

confidence: 99%

“…In rodents and humans (Ludwig et al 1994;Occhiodoro et al 1998;Pardo et al 1999;Hemmerlein et al 2006), Eag1 channels are mainly expressed in the adult brain where their function is still unknown. Several studies (Ludwig et al 2000;Saganich et al 2001 andMartin et al 2008) reported a mapping of Eag1, where the highest Eag1 expression levels occur in the olfactory bulb, cerebral cortex, hippocampus, cerebellum, and hypothalamus of the adult rat. However, due to the lack of specific pharmacological agents, the precise neurophysiological role of Eag1 in mammalian neurons remains elusive.…”

Section: Introductionmentioning

confidence: 99%

Ether-à-go-go 1 (Eag1) Potassium Channel Expression in Dopaminergic Neurons of Basal Ganglia is Modulated by 6-Hydroxydopamine Lesion

et al. 2011

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The ether à go-go (Eag) gene encodes the voltage-gated potassium (K(+)) ion channel Kv10.1, whose function still remains unknown. As dopamine may directly affect K(+) channels, we evaluated whether a nigrostriatal dopaminergic lesion induced by the neurotoxin 6-hydroxydopamine (6-OHDA) would alter Eag1-K(+) channel expression in the rat basal ganglia and related brain regions. Male Wistar rats received a microinjection of either saline or 6-OHDA (unilaterally) into the medial forebrain bundle. The extent of the dopaminergic lesion induced by 6-OHDA was evaluated by apomorphine-induced rotational behavior and by tyrosine hydroxylase (TH) immunoreactivity. The 6-OHDA microinjection caused a partial or complete lesion of dopaminergic cells, as well as a reduction of Eag1+ cells in a manner proportional to the extent of the lesion. In addition, we observed a decrease in TH immunoreactivity in the ipsilateral striatum. In conclusion, the expression of the Eag1-K(+)-channel throughout the nigrostriatal pathway in the rat brain, its co-localization with dopaminergic cells and its reduction mirroring the extent of the lesion highlight a physiological circuitry where the functional role of this channel can be investigated. The Eag1-K(+) channel expression in dopaminergic cells suggests that these channels are part of the diversified group of ion channels that generate and maintain the electrophysiological activity pattern of dopaminergic midbrain neurons.

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Eag1 potassium channel immunohistochemistry in the CNS of adult rat and selected regions of human brain

Cited by 59 publications

References 35 publications

Eag1 potassium channels as markers of cervical dysplasia

Eag1 potassium channels as markers of cervical dysplasia

Surface dynamics of voltage-gated ion channels

Ether-à-go-go 1 (Eag1) Potassium Channel Expression in Dopaminergic Neurons of Basal Ganglia is Modulated by 6-Hydroxydopamine Lesion

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