E6/E7 of HPV Type 16 Promotes Cell Invasion and Metastasis of Human Breast Cancer Cells

Yasmeen, Amber; Bismar, Tarek A.; Kandouz, Mustapha; Foulkes, William D.; Desprez, Pierre-Yves; Moustafa, Ala‐Eddin Al

doi:10.4161/cc.6.16.4555

Cited by 87 publications

(95 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Separately, we reported that the presence of E6/E7 of HPV type 16 is correlated with Id-1 overexpression in human invasive and metastatic breast cancer tissues in Canadian women. Moreover, we revealed that E6/E7 of HPV type 16 affects Id-1 deregulation through the activation of its promoter (Yasmeen et al, 2007a). Therefore, the present study clearly suggests that Id-1 is the downstream target of E6/E7 of HPV types 18, 31, 33 and 35 in human breast cancer progression.…”

Section: Discussionmentioning

confidence: 58%

“…Moreover, we demonstrated that E6/E7 onco-proteins of HPV type 16 convert non-invasive breast cancer cells to an invasive phenotype (Yasmeen et al, 2007a); this is accompanied by an overexpression of Id-1, which regulates cell invasion and metastasis of human breast cancer cells (Desprez et al, 1998;Fong et al, 2003). In this study, we investigated the relation between high-risk HPVs and Id-1 expression in breast cancer tissues from Syrian women.…”

Section: Discussionmentioning

confidence: 99%

“…Regarding the association between high-risk HPV and tumour aggressiveness, recent studies, including ours, have reported that the presence of HPV type 16, in human breast cancer, is correlated with invasive carcinomas (Kroupis et al, 2006;Yasmeen et al, 2007a). Moreover, we demonstrated that E6/E7 onco-proteins of HPV type 16 convert non-invasive breast cancer cells to an invasive phenotype (Yasmeen et al, 2007a); this is accompanied by an overexpression of Id-1, which regulates cell invasion and metastasis of human breast cancer cells (Desprez et al, 1998;Fong et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, studies that found HPV-positive samples revealed that certain types of high-risk HPV infections are linked to specific geographic locations. According to this observation, we recently reported that HPV type 16 is the only type of high-risk HPV present in breast cancer tissues of Canadian women (Yasmeen et al, 2007a).…”

mentioning

confidence: 99%

See 3 more Smart Citations

High-risk human papillomavirus infections in breast cancer in Syrian women and their association with Id-1 expression: a tissue microarray study

et al. 2008

View full text Add to dashboard Cite

High-risk human papillomaviruses (HPVs) could be important risk factors for breast carcinogenesis and metastasis. Based on this hypothesis, we recently studied the effect of E6/E7 onco-proteins of high-risk HPV type 16 in two non-invasive human breast cancer cell lines, BT20 and MCF7; we reported that E6/E7 converts these cell lines to invasive cells. This is accompanied by an overexpression of Id-1, which is an important regulator of breast metastasis. In this investigation, we examined the presence of highrisk HPVs (16, 18, 31, 33 and 35) and the expression of their E6 onco-protein as well as their correlation with Id-1 gene expression, using polymerase chain reaction (PCR) and tissue microarray (TMA) analysis, respectively, in a cohort of 113 Syrian breast cancer patients. We found that high-risk HPV types 16, 18, 31, 33 and 35 are present in 8.84, 9.73, 7.07, 55.75 and 37.16% of our samples, respectively, which represent invasive breast cancers. Overall, 69 (61.06%) of the 113 samples are HPV positive; among these specimens 24 tissues (34.78%) are coinfected with more than one HPV type. Furthermore, we report that the expression of the E6 onco-protein of these high-risk HPVs is correlated with Id-1 overexpression in the majority of invasive breast cancer tissue samples. Our data suggest that high-risk HPV infections are associated with human breast cancer progression in Syrian women.

show abstract

Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

High-risk human papillomavirus infections in breast cancer in Syrian women and their association with Id-1 expression: a tissue microarray study

et al. 2008

View full text Add to dashboard Cite

show abstract

“…Furthermore, we found that HPV type 16 is present in all invasive and metastatic breast cancers and less frequently in in-situ breast cancer in contrast to normal mammary tissue. 14 Recently, Woods Ignatoski et al reported that E6/E7 of HPV type 16 and ErB-2 cooperate with endogenous epidermal growth factor-receptor (EGF-R/ErbB1) to fully transform human breast immortalized cell line, MCF-10A. 15 Our group has developed a new in vitro model to study the effects of E6/E7 of HPV type 16 and ErbB-2 in human normal oral epithelial (NOE) cells.…”

Section: Introductionmentioning

confidence: 99%

ErbB-2 Receptor Cooperates with E6/E7 Oncoproteins of HPV Type 16 in Breast Tumorigenesis

Yasmeen

Bismar²,

Dekhil³

et al. 2007

Cell Cycle

Self Cite

View full text Add to dashboard Cite

Previously published online as a Cell Cycle E-publication: http://www.landesbioscience.com/journals/cc/article/4949 KEY woRDsBreast tumorigenesis, transgenic mice, ErbB-2 and E6/E7 of high-risk HPV ACKnowlEDGEMEnTsWe are grateful to Drs G. Batist, M. AlaouiJamali and Foulkes for their support of this work. We are thankful to Dr. A. Darnel for his critical reading of the manuscript. We also thank Dr. T. Li-Qun and G. Mousavi for their technical assistance. This work is supported by the Canadian Institutes for Health Research (Ala-Eddin Al Moustafa) and the National Colorectal Cancer Campaign (Ala-Eddin Al Moustafa and Tarek A. Bismar). ABsTRACTThe ErbB-2 receptor is over-expressed in roughly 30% of human breast cancers. Moreover, approximately 50% of breast cancers are positive for high-risk human papillomaviruses (HPVs). Recently, we reported that ErbB-2 cooperates with E6/E7 oncoproteins of HPV type 16 to induce neoplastic transformation of human normal oral epithelial cells. We also demonstrated that E6/E7 of HPV type 16 converts noninvasive breast cancer cells to an invasive form. In order to investigate the effect of ErbB-2/E6/E7 cooperation in breast carcinogenesis, we generated double transgenic mice carrying ErbB-2 and E6/E7 of HPV type 16 under mouse mammary tumor virus (MMTV) and human keratin 14 promoters, respectively. Within six months, these double transgenic mice developed large and extensive invasive breast cancer in comparison to ErbB-2 or E6/E7 singly transgenic mice. Histological analysis of ErbB-2/ E6/E7 transgenic mice tumors showed the presence of invasive breast carcinomas. However, the breast tissues from ErbB-2 and E6/E7 transgenic mice showed only in-situ cancer and normal mammary phenotype, respectively. In parallel, we examined the cooperation effect of ErbB-2 and E6/E7 in the human breast cancer cell line, BT20; in comparison to ErbB-2 and E6/E7 alone as well as wild type cells, we found that ErbB 2/E6/E7 together stimulate colony formation and cell migration in the BT20 cell line. Furthermore, we found that b-catenin is constitutively phosphorylated by c-Src and consequently trans-located to the nucleus in ErbB-2/E6/E7-breast cancer cells. These findings provide evidence that the ErbB-2 receptor cooperates with high-risk HPVs in breast tumorigenesis via b-catenin activation.

show abstract

Cyclooxygenase‐2 expression and clinical parameters in laryngeal squamous cell carcinoma, vocal fold nodule, and laryngeal atypical hyperplasia

Sayar

Özdemir

et al. 2012

Head & Neck

View full text Add to dashboard Cite

show abstract

E6/E7 of HPV Type 16 Promotes Cell Invasion and Metastasis of Human Breast Cancer Cells

Cited by 87 publications

References 19 publications

High-risk human papillomavirus infections in breast cancer in Syrian women and their association with Id-1 expression: a tissue microarray study

High-risk human papillomavirus infections in breast cancer in Syrian women and their association with Id-1 expression: a tissue microarray study

ErbB-2 Receptor Cooperates with E6/E7 Oncoproteins of HPV Type 16 in Breast Tumorigenesis

Cyclooxygenase‐2 expression and clinical parameters in laryngeal squamous cell carcinoma, vocal fold nodule, and laryngeal atypical hyperplasia

Contact Info

Product

Resources

About