“…Otx2, sox3, and sox10 factors, involved in NC differentiation and neural development, may not co-operate with foxd3 to mediate repression but rather compete for underlying binding sequences to promote multipotent NC cell differentiation (Beby and Lamonerie, 2013, Carney et al., 2006, Dee et al., 2008). Conversely, e2f6 factor is known to function as a transcriptional repressor that associates with Polycomb repressive complexes (PRC1 and PRC2) (Gaubatz et al., 1998, Leseva et al., 2013, Trimarchi et al., 2001). We explored e2f6/foxd3 co-operation in transcriptional repression by scanning four different e2f6 motifs across all foxd3-bound regions associated with the genes upregulated in 14ss foxd3 -mutant embryos (Figure 3E) and found that 82.3% of them were significantly enriched for e2f6 binding (chi-square test; ∗∗∗ p < 0.0004).…”