E2F4 regulates cell cycle to mediate embryonic development in pigs

Jiang, Wen-Jie; Sun, Ming‐Hong; Li, Xiaohan; Lee, Songhee; Heo, Geun; Zhou, Dongjie; Cui, Xiang-Shun

doi:10.1016/j.theriogenology.2022.10.040

Cited by 3 publications

(3 citation statements)

References 36 publications

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“…Quantitative reverse transcription-PCR was performed using a fast real-time PCR system (ABI StepOnePlus). Real-time quantitative PCR (qPCR) was performed according to previous article ( Jiang et al, 2023a ). 18S rRNA was used as the reference gene.…”

Section: Methodsmentioning

confidence: 99%

Knockdown of Y-box binding protein 1 induces autophagy in early porcine embryos

Jiang,

Lee,

Heo

et al. 2023

Front. Cell Dev. Biol.

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Y-box binding protein 1 (YBX1) plays important roles in RNA stabilization, translation, transcriptional regulation, and mitophagy. However, its effects on porcine preimplantation embryos remain unclear. In this study, we knocked down YBX1 in the one-cell (1C) stage embryo via small interfering RNA microinjection to determine its function in porcine embryo development. The mRNA level of YBX1 was found to be highly expressed at the four-cell (4C) stage in porcine embryos compared with one-cell (1C) and two-cell (2C) stages. The number of blastocysts was reduced following YBX1 knockdown. Notably, YBX1 knockdown decreased the phosphatase and tensin homolog-induced kinase 1 (PINK1) and parkin RBR E3 ubiquitin protein ligase (PRKN) mRNA levels. YBX1 knockdown also decreased PINK1, active mitochondria, and sirtuin 1 levels, indicating reduced mitophagy and mitochondrial biogenesis. Furthermore, YBX1 knockdown increased the levels of glucose-regulated protein 78 (GRP78) and calnexin, leading to endoplasmic reticulum (ER) stress. Additionally, YBX1 knockdown increased autophagy and apoptosis. In conclusion, knockdown of YBX1 decreases mitochondrial function, while increasing ER stress and autophagy during embryonic development.

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Section: Methodsmentioning

confidence: 99%

Knockdown of Y-box binding protein 1 induces autophagy in early porcine embryos

Jiang,

Lee,

Heo

et al. 2023

Front. Cell Dev. Biol.

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“…For real‐time reverse transcription‐polymerase chain reaction (RT‐PCR), we used a previously reported method 25 . Briefly, mRNA from 30 to 50 blastocysts per group was extracted using the DynaBeads mRNA Direct Kit (61012; Thermo Fisher Scientific), according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

“…For real-time reverse transcription-polymerase chain reaction (RT-PCR), we used a previously reported method. 25 Briefly, mRNA from 30 to 50 blastocysts per group was extracted using the DynaBeads mRNA Direct Kit (61012; Thermo Fisher Scientific), according to the manufacturer's instructions. cDNA was obtained via reverse transcription of mRNA using Oligo (dT) 20 primer and SuperScript III Reverse Transcriptase (Thermo Fisher Scientific).…”

Section: Real-time Reverse Transcription-polymerase Chain Reactionmentioning

confidence: 99%

GRP78 acts as a cAMP/PKA signaling modulator through the MC4R pathway in porcine embryonic development

Heo,

Lee,

Kim

et al. 2023

The FASEB Journal

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Glucose‐regulated protein 78 (GRP78) binds to and stabilizes melanocortin 4 receptor (MC4R), which activates protein kinase A (PKA) by regulating G proteins. GRP78 is primarily used as a marker for endoplasmic reticulum stress; however, its other functions have not been well studied. Therefore, in this study, we aimed to investigate the function of GRP78 during porcine embryonic development. The developmental quality of porcine embryos, expression of cell cycle proteins, and function of mitochondria were evaluated by inhibiting the function of GRP78. Porcine oocytes were activated to undergo parthenogenesis, and blastocysts were obtained after 7 days of in vitro culture. GRP78 function was inhibited by adding 20 μM HA15 to the in vitro culture medium. The inhibition in GRP78 function led to a decrease in G proteins release, which subsequently downregulated the cyclic adenosine monophosphate (cAMP)/PKA pathway. Ultimately, inhibition of GRP78 function induced the inhibition of CDK1 and cyclin B expression and disruption of the cell cycle. In addition, inhibition of GRP78 function regulated DRP1 and SIRT1 expression, resulting in mitochondrial dysfunction. This study provides new insights into the role of GRP78 in porcine embryonic development, particularly its involvement in the regulation of the MC4R pathway and downstream cAMP/PKA signaling. The results suggest that the inhibition of GRP78 function in porcine embryos by HA15 treatment may have negative effects on embryo quality and development. This study also demonstrated that GRP78 plays a crucial role in the functioning of MC4R, which releases the G protein during porcine embryonic development.

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Proteomic analysis reveals that cigarette smoke exposure diminishes ovarian reserve in mice by disrupting the CREB1-mediated ovarian granulosa cell proliferation-apoptosis balance

Xu,

Li,

et al. 2024

Ecotoxicology and Environmental Safety

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E2F4 regulates cell cycle to mediate embryonic development in pigs

Cited by 3 publications

References 36 publications

Knockdown of Y-box binding protein 1 induces autophagy in early porcine embryos

Knockdown of Y-box binding protein 1 induces autophagy in early porcine embryos

GRP78 acts as a cAMP/PKA signaling modulator through the MC4R pathway in porcine embryonic development

Proteomic analysis reveals that cigarette smoke exposure diminishes ovarian reserve in mice by disrupting the CREB1-mediated ovarian granulosa cell proliferation-apoptosis balance

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