E2F Transcription Factors in Cancer, More than the Cell Cycle

Broeker, Carson D.; Andrechek, Eran R.

doi:10.1016/b978-0-12-820472-6.00102-x

Cited by 1 publication

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“…A large number of genes that code for these proteins have promoter sequences recognized by the transcription factor E2F-4. E2F is a family of transcription factors that are involved in cell cycle regulation and have several effects on the intrinsic apoptosis pathway [ 51 , 52 , 53 ]. Moreover, Garneau et al [ 54 ] reported that nuclear expression of E2F-4 induces cell death via multiple pathways in normal human intestinal epithelia.…”

Section: Discussionmentioning

confidence: 99%

Effects of Erythrodiol on the Antioxidant Response and Proteome of HepG2 Cells

et al. 2021

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Erythrodiol (EO) is a pentacyclic triterpenic alcohol found in olive tree leaves and olive oil, and it has important effects on the health properties and quality of olive oil. In this study, we characterized the cytotoxic effects of EO on human hepatocarcinoma (HepG2) cells by studying changes in cell viability, reactive oxygen species (ROS) production, antioxidant defense systems, and the proteome. The results reveal that EO markedly decreased HepG2 cell viability without changing ROS levels. The concentrations of glutathione and NADPH were significantly reduced, with selective changes in the activity of several antioxidant enzymes: glutathione peroxidase, glutathione reductase, glucose 6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase. Proteomic data reveal that EO led to the complete elimination or decreased abundance of 41 and 3 proteins, respectively, and the abundance of 29 proteins increased. The results of functional enrichment analysis show that important metabolic processes and the nuclear transport of mature mRNA were impaired, whereas AMP biosynthesis and cell cycle G2/M phase transition were induced. The transcription factors and miRNAs involved in this response were also identified. These potent antiproliferative effects make EO a good candidate for the further analysis of its hepatic antitumor effects in in vivo studies.

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