e0177 Exploration new methods for establishment of porcine model of acute myocardial infarction

Abstract

Aims To investigate the effect of HIF-1a on MSC under hypoxia condition. Materials and methods We transfected HIF-1a into MSC of P3 generation through liposome 2000, and observed the expression of green fluorescence protein in order to assess transfecting efficiency. G418 was used to screen stable transfected cells, and limited dilution method used for monoclone culture of screened cells. We identified the stable HIF-1a transfected MSC through the cell surface antigen testing. We compared the growth state among stable transfected MSC with HIF-1a, vacant plasmid transfected MSC and untransfected MSC under hypoxia condition, and the expression of HIF-1a mRNA, VEGF mRNA, HIF-1a protein and VEGF protein was tested. Objective To observe the effect of ghrelin on reducing the apoE À/À mice plasma IL-8, MCP-1, TNFa level and the NFkBp65 expression in vascular wall and the regression of atherosclerotic plaque. Method 8 week ApoE À/À mice were fed with Western style meals, and the same age mice C57BL/6J fed with the same meals as control. In the eighth week, ApoE À/À mice were assigned to ghrelin intraperitoneal injection and saline injection group randomly in the twelfth week.All of the groups had blood drawn from eye sockets, with isolated plasma used to measure IL-8, MCP-1, TNFa by ELISA. Mice were killed for examination with stereomicroscopy and paraffin imbedding for HE and immunohistochemistry, and frozen section for red oil stain. Objective The aim of this study was to investigate the effects of rosuvastatin on the expression of homocysteine-induced expression of matrix metalloproteinase-2 (MMP-2) and cell migration in rat vascular smooth muscle cells (VSMC).Methods Cultured rat VSMC were incubated with different concentrations of Hcy and rosuvastatin (Hcy 1000 mmol/l) in vitro for 24, 48 and 72 h. The expression of MMP-2 was determined by using the methods of gelatin zymography and western blotting. Objective To explore and develop one optimise method that it could establish the porcine model of acute myocardial infraction more safer, quicker, convenient than routine methods. Methods 30 animals with health condition, mean weight 26.564.8 kg; The pigs were divided into two groups randomly, group A (n¼13) and group B (n¼17), according to different method. Angioplasty balloon was positioned in the mid-distal of left anterior