E-β-farnesene synergizes the influence of an insecticide to improve control of cabbage aphids in China

Cui, Liang-Liang; Dong, Jie; Francis, Frédéric; Liu, Yingjie; Heuskin, Stéphanie; Lognay, Georges; Chen, Julian; Bragard, Claude; Tooker, John F.; Liu, Yong

doi:10.1016/j.cropro.2012.01.003

Cited by 23 publications

(25 citation statements)

References 45 publications

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“…When under predator attack, the cornicles of aphids secrete droplets containing alarm pheromone to warn their conspecifics of potential threats, the main component of which is the sesquiterpene (E)-βfarnesene (EβF) (Pickett and Griffiths, 1980;Pickett et al, 1992;Francis et al, 2005). Besides its alarm activity, EβF also showed insecticidal activity at high doses (Qin et al, 2016), and could be used as a synergistic agent for controlling aphids when mixed with commercial insecticides (Cui et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

The terpenoid backbone biosynthesis pathway directly affects the biosynthesis of alarm pheromone in the aphid

Sun

2018

Insect Molecular Biology

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The terpenoid backbone biosynthesis pathway is responsible for the synthesis of different backbones for terpenoids; (E)-β-farnesene (EβF), a sesquiterpene, is the major component of aphid alarm pheromone. Our previous studies eliminated the possibility of host plants and endosymbionts as the sources of EβF, and we thus speculate that the terpenoid pathway might affect the biosynthesis of EβF in aphids. First, the transcriptional responses of four genes encoding farnesyl diphosphate synthase (FPPS), geranylgeranyl diphosphate synthase (GGPPS) and decaprenyl diphosphate synthase in the cotton aphid Aphis gossypii to simulated stimulation were analysed using quantitative real-time PCR, showing an immediate decrease in the transcript abundances of the four genes. Next, RNA-interference-mediated gene knockdown was performed, indicating that fpps knockdown caused a significant cost in terms of body size and fecundity. Finally, an association analysis of gene knockdown with the amount of EβF was conducted, revealing that the concentration of EβF per milligram of aphid was drastically decreased in response to fpps knockdown, whereas ggpps knockdown significantly raised the concentration of EβF. Our data support a peculiar mode of biosynthesis and storage of the aphid alarm pheromone that relies directly on the terpenoid backbone biosynthesis pathway in the aphid.

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Section: Introductionmentioning

confidence: 99%

The terpenoid backbone biosynthesis pathway directly affects the biosynthesis of alarm pheromone in the aphid

Sun

2018

Insect Molecular Biology

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“…2 More importantly, since EBF is used by the majority of aphid species as an alarm pheromone, 3 this sesquiterpene is a valuable chemical to control aphid pests in crops. 2a,4 To date, cDNAs coding for EBFS have been isolated from several plants, 4g,5 and some have been over-expressed in bacterial 2a, 6 and plant hosts. 2b,4f,g,7 The amino acid sequence of EBFSs, 2a amino acid sequence alignments 4h,5b,6a,c–e and molecular modeling suggest that EBFSs possess the characteristic class I terpene fold found in all sesquiterpene synthases.…”

Section: Introductionmentioning

confidence: 99%

“…2a,4 To date, cDNAs coding for EBFS have been isolated from several plants, 4g,5 and some have been over-expressed in bacterial 2a, 6 and plant hosts. 2b,4f,g,7 The amino acid sequence of EBFSs, 2a amino acid sequence alignments 4h,5b,6a,c–e and molecular modeling suggest that EBFSs possess the characteristic class I terpene fold found in all sesquiterpene synthases. 1c EBFS from Mentha x piperita has the diagnostic Asp-rich DDXXD motif (residues 301-305) that coordinates essential Mg 2+ -ions, and the non-catalytic N-terminal domain found in most plant-derived sesquiterpene synthases.…”

Section: Introductionmentioning

confidence: 99%

Probing the Mechanism of 1,4-Conjugate Elimination Reactions Catalyzed by Terpene Synthases

Faraldos

González

et al. 2012

J. Am. Chem. Soc.

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The reaction mechanisms of (E)-β-farnesene synthase (EBFS) and isoprene synthase (ISPS), enzymes that catalyze a formal regioespecific 1,4-conjugate elimination of hydrogen-diphosphate from (E, E)-farnesyl and dimethylallyl diphosphate (FDP and DMADP) to generate the semiochemicals (E)-β-farnesene and isoprene, respectively, were probed with substrate analogs and kinetic measurements. The results support stepwise reaction mechanisms through analogous enzyme-bound allylic cationic intermediates. For EBFS, we demonstrate that the elimination reaction can proceed via the enzyme-bound intermediate trans-nerolidyl diphosphate, while for ISPS the intermediacy of 2-methylbut-3-enyl 2-diphosphate can be inferred from the product outcome when deuterated DMADPs are used as substrates. Possible implications derived from the mechanistic details of the EBFS catalyzed reaction for the evolution of sesquiterpene synthases are discussed.

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“…Although chemical control is effective, overreliance on this method carries health and environmental risks associated with the residues that remain on plant leaves, along with the resistance that herbivores may develop, which in turn may lead to the resurgence of primary pests and outbreaks of secondary pests [10] . Therefore, it is prudent to explore alternatives to chemical control to improve the sustainability of pest management [11] . Methods that alter herbivore behavior or manipulate populations of natural enemies have been recognized as potential alternative tools for the control of insect pest populations [12] .…”

Section: Introductionmentioning

confidence: 99%

The influence of Rosemary plants (Rosmarinus officinalis) volatiles on Aphid (Myzus Persicae)

Cai¹,

Wang²,

Zhang³

et al. 2018

Proceedings of the 2017 3rd International Forum on Energy, Environment Science and Materials (IFEESM 2017)

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Volatile organic compounds (VOCs) can have an important influence on plant-insect interactions. In particular, insect behaviors are often influenced by the volatiles of both host plants and non-host plants. The objective of this study was to assess the behavioral responses of the aphid, Myzus persicae (Sulzer) to the volatiles of the woody perennial herb Rosemary ( Rosmarinus officinalis, family Lamiaceae). Our studies using a range doses of plants have shown that, at all of the different doses tested except the weight of rosemary was 0.5 g, volatiles emitted from rosemary plants showed repellent effects on M. persicae. These findings provide new insights into the interactions between R. officinalis plants and M. persicae, which will contribute to the development of new management strategies for biological control of insect pests

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E-β-farnesene synergizes the influence of an insecticide to improve control of cabbage aphids in China

Cited by 23 publications

References 45 publications

The terpenoid backbone biosynthesis pathway directly affects the biosynthesis of alarm pheromone in the aphid

The terpenoid backbone biosynthesis pathway directly affects the biosynthesis of alarm pheromone in the aphid

Probing the Mechanism of 1,4-Conjugate Elimination Reactions Catalyzed by Terpene Synthases

The influence of Rosemary plants (Rosmarinus officinalis) volatiles on Aphid (Myzus Persicae)

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