E. coli RNA polymerase interacts homologously with two different promoters

Siebenlist, Ulrich; Simpson, Robert B.; Gilbert, Walter

doi:10.1016/0092-8674(80)90613-3

Cited by 902 publications

(473 citation statements)

References 17 publications

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“…Futhermore, three nucleotides 5' of ATG is no G and the positions 5 to 10 3' of A from the ATG are rich in A and T. These homologies to the genes of E. coli indicate that Acinetobacter uses similar signals for the initiation of translation. This lends additional support to the assumption that the ATG in position 121 (see Figure 2) is indeed the start codon for the mutarotase precursor, The initiation site of transcription is not obvious from homologies with E. coli promotors (41). No Table III).…”

Section: Discussionmentioning

confidence: 64%

Acinetobacter calcoaceticusencoded mutarotase: nucleotide sequence analysis of the gene and characterization of its secretion inEscherichia coli

Gatz

Hillen

1986

Nucl Acids Res

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Section: Discussionmentioning

confidence: 64%

Acinetobacter calcoaceticusencoded mutarotase: nucleotide sequence analysis of the gene and characterization of its secretion inEscherichia coli

Gatz

Hillen

1986

Nucl Acids Res

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“…This region is 241 bases upstream of the initiation codon for ORF2, which is the longest open reading frame in IS66. The sequence T-G-T-C-A-T, found at position 870 in the upper strand, is also found in the -10 region of the promoter for the araC gene of E. coli (30). The sequence A-T-G-A-C-A, which is located 21 bases upstream of the -10 region, resembles the consensus sequence of the -35 region, T-T-G-A-C-A, in the E. coli promoter (30).…”

Section: Discussionmentioning

confidence: 99%

“…The sequence T-G-T-C-A-T, found at position 870 in the upper strand, is also found in the -10 region of the promoter for the araC gene of E. coli (30). The sequence A-T-G-A-C-A, which is located 21 bases upstream of the -10 region, resembles the consensus sequence of the -35 region, T-T-G-A-C-A, in the E. coli promoter (30). No other obvious promoter sequence was found between this region and the initiation codon of ORF2.…”

Section: Discussionmentioning

confidence: 99%

Nucleotide sequence of the insertion sequence found in the T-DNA region of mutant Ti plasmid pTiA66 and distribution of its homologues in octopine Ti plasmid.

Machida¹,

Sakurai²,

Kiyokawa³

et al. 1984

Proc. Natl. Acad. Sci. U.S.A.

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The octopine tumor-inducing (Ti) plasmid pTiA66 has an insertion mutation in its T region (the DNA region incorporated into the plant genome) that results in the slow growth of crown gall tumors. These tumors exhibit hormonal autonomy different from that of the crown gall tumors caused by wild-type Ti plasmids. In the present study, the nucleotide sequences of both the DNA segment inserted into pTiA66 and its target site have been determined. The inserted segment is 2548 base pairs long and has 20-base-pair terminal inverted repeats. An 8-base-pair sequence at the target site is duplicated at both integration junctions. These structural features of the insert suggest that it is a bacterial insertion sequence (IS) element, which we have named IS66. Blot-hybridization analyses using IS66 probes revealed that genomes of octopine Ti plasmids contain at least three sequences homologous to IS66: two homologues are located in the virulence region and one is located between the left-hand (TL-DNA) and right-hand (TR-DNA) portions of T-DNA. The chromosome of Agrobacterium tumefaciens A66 also contains two sequences highly homologous to IS66. These results suggest that the mutant pTiA66 plasmid was generated by translocation of one of the sequences showing homology with IS66 into the T region. The fact that a sequence homologous to IS66 is present between TL-DNA and TR-DNA also suggests that the octopine T region was split into two portions, TL-DNA and TR-DNA, by translocation of IS66 or its relatives. Thus, IS66 may cause genetic and structural variations of the T region and the vir region of the octopine Ti plasmids.Tumor-inducing (Ti) plasmids harbored by oncogenic strains of Agrobacterium tumefaciens cause formation of tumors on dicotyledonous plants, called crown galls (1). Cells isolated from the crown gall tumors are characterized by their unlimited proliferation in medium lacking phytohormones, such as cytokinins and auxins, that are required for the culture of normal plant cells (2). It has been shown that a specific DNA region of the Ti plasmid, called the T-DNA region (Fig. 1), causes tumor formation when transferred into plant chromosomes and that the T-DNA persists in the chromosomes of the tumor cells (3-6). The virulence (vir) region of the Ti plasmid (Fig. 1) is located outside the T region and is also required for tumorigenesis by the Ti plasmid. However, this region is probably not stably maintained in the tumor cells (7-10).The Ti plasmids are classified on the basis of the novel amino acid metabolites, such as octopine or nopaline, whose syntheses are directed by T-DNA genes in the tumor cells (11-15). The octopine T region is composed of twQ DNA segments near each other in the Ti plasmid (16) (Fig. 1), whereas the nopaline T region consists of one contiguous DNA segment (17, 18). The chromosomes of the tumor cells caused by the octopine strain contain either one or two portions of the T region (16). All tumor cells that have been analyzed contained the left portion of T-DNA (TL-DNA) but not all...

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“…Confirmation of the role of these sequences awaits the purification and sequencing of the argE and argC gene products, (33) showing that a stretch of DNA, ranging from positions -48 to +25 on Figure 4, was protected by RNA polymerase against (32).…”

Section: Introductionmentioning

confidence: 99%

The regulatory region of the divergentargECBHoperon inEscherichia coliK-12

et al. 1982

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The nucleotide sequence of the control region of the divergent argECBH operon has been established in the wild type and in mutants affecting expression of these genes. The argE and argCBH promoters face each other and overlap with an operator region containing two domains which may act as distinct repressor binding sites. A long leader sequence -not involved in attenuation -precedes argCBH. Overlapping of the argCBH promoter and the region involved in ribosome mobilization for argE translation explains the dual effect of some mutations. Mutations causing semi-constitutive expression of argE improve putative promoter sequences within argC. Implications of these results regarding control mechanisms in amino acid biosynthesis and their evolution are discussed.

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E. coli RNA polymerase interacts homologously with two different promoters

Cited by 902 publications

References 17 publications

Acinetobacter calcoaceticusencoded mutarotase: nucleotide sequence analysis of the gene and characterization of its secretion inEscherichia coli

Acinetobacter calcoaceticusencoded mutarotase: nucleotide sequence analysis of the gene and characterization of its secretion inEscherichia coli

Nucleotide sequence of the insertion sequence found in the T-DNA region of mutant Ti plasmid pTiA66 and distribution of its homologues in octopine Ti plasmid.

The regulatory region of the divergentargECBHoperon inEscherichia coliK-12

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