E. coli electroeradication on a closed loop circuit by using milli-, micro- and nanosecond pulsed electric fields: Comparison between energy costs

Guionet, Alexis; David, Fabienne; Zaepffel, Clément; Coustets, Mathilde; Helmi, Karim; Cheype, C.; Packan, Denis; Garnier, Jean‐Pierre; Blanckaert, Vincent; Teissié, Justin

doi:10.1016/j.bioelechem.2014.08.021

Cited by 28 publications

(11 citation statements)

References 37 publications

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“…In the present study we show in vitro results for the efficacy of 600-ns PEF to inactivate Escherichia coli and Lactobacillus acidophilus. While the effects of nsPEF have been studied on E. coli (Chalise et al 2006;Perni et al 2007;Guionet et al 2014Guionet et al , 2015Novickij et al 2018) and other species such as Staphylococcus aureus (Chaturongakul and Kirawanich 2012; Vadlamani et al 2018;Novickij et al 2019), Salmonella typhimurium (Perni et al 2007) and Bacillus subtilis (Katsuki et al 2002); we chose to compare the effects of E. coli with L. acidophilus for two reasons. First, several studies have suggested that cell size and shape play a critical role in transmembrane potential charging and subsequent membrane pore formation (Kandušer and Miklavčič 2008;Khan and El-Hag 2011).…”

Section: Discussionmentioning

confidence: 99%

600-ns pulsed electric fields affect inactivation and antibiotic susceptibilities of Escherichia coli and Lactobacillus acidophilus

et al. 2020

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Cell suspensions of Escherichia coli and Lactobacillus acidophilus were exposed to 600-ns pulsed electric fields (nsPEFs) at varying amplitudes or High-23.5 kV cm −1 ) and pulse numbers (0 (sham), 1, 5, 10, 100 or 1000) at a 1 hertz (Hz) repetition rate. The induced temperature rise generated at these exposure parameters, hereafter termed thermal gradient, was measured and applied independently to cell suspensions in order to differentiate inactivation triggered by electric field (E-field) from heating. Treated cell suspensions were plated and cellular inactivation was quantified by colony counts after a 24-hour (h) incubation period. Additionally, cells from both exposure conditions were incubated with various antibiotic-soaked discs to determine if nsPEF exposure would induce changes in antibiotic susceptibility. Results indicate that, for both species, the total delivered energy (amplitude, pulse number and pulse duration) determined the magnitude of cell inactivation. Specifically, for 18.5 and 23.5 kV cm −1 exposures, L. acidophilus was more sensitive to the inactivation effects of nsPEF than E. coli, however, for the 13.5 kV cm −1 exposures E. coli was more sensitive, suggesting that L. acidophilus may need to meet an E-field threshold before significant inactivation can occur. Results also indicate that antibiotic susceptibility was enhanced by multiple nsPEF exposures, as observed by increased zones of growth inhibition. Moreover, for both species, a temperature increase of ≤ 20 °C (89% of exposures) was not sufficient to significantly alter cell inactivation, whereas none of the thermal equivalent exposures were sufficient to change antibiotic susceptibility categories.

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Section: Discussionmentioning

confidence: 99%

600-ns pulsed electric fields affect inactivation and antibiotic susceptibilities of Escherichia coli and Lactobacillus acidophilus

et al. 2020

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“…He-O 2 plasma is the most efficient plasma for bacterial inactivation and seems to involve an electric field associated with the ionization front or, generated in the plasma environment. The inactivation of bacteria by electrochemical means has been well characterized [ 59 ]. It involves different mechanisms such as changes in the membrane potential which surely may lead to local ion flux imbalances.…”

Section: Discussionmentioning

confidence: 99%

Oxidative modification and electrochemical inactivation of Escherichia coli upon cold atmospheric pressure plasma exposure

et al. 2017

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Cold atmospheric pressure plasmas (CAPPs) are known to have bactericidal effects but the mechanism of their interaction with microorganisms remains poorly understood. In this study the bacteria Escherichia coli were used as a model and were exposed to CAPPs. Different gas compositions, helium with or without adjunctions of nitrogen or oxygen, were used. Our results indicated that CAPP induced bacterial death at decontamination levels depend on the duration, post-treatment storage and the gas mixture composition used for the treatment. The plasma containing O2 in the feeding gas was the most aggressive and showed faster bactericidal effects. Structural modifications of treated bacteria were observed, especially significant was membrane leakage and morphological changes. Oxidative stress caused by plasma treatment led to significant damage of E. coli. Biochemical analyses of bacterial macromolecules indicated massive intracellular protein oxidation. However, reactive oxygen and nitrogen species (RONS) are not the only actors involved in E. coli’s death, electrical field and charged particles could play a significant role especially for He-O2 CAPP.

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“…As such, our system is adaptable as a continuous oil and polysaccharide extraction process at low energy cost. In the flow treatment process, electric field application to treat large volumes was examined for bacterial eradication and protein extraction from microalgae [ 9 , 10 , 12 , 40 ]. In particular, it is possible to design a system using a derivation to separate algae culture from a bioreactor, treat it by nsPEF, extract oil and polysaccharide by in-line decantation (lamellar decanter might help to lead single cells on the bottom part and matrix on the upper part), and then return the medium and surviving algae to the bioreactor, as shown in Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Once permeabilized, some intracellular elements may be extracted from cells; protein, for example, can be extracted from intracellular algae using PEF [ 9 – 11 ]. Regarding pulse length, nanosecond pulsed electric fields (nsPEF) have been shown to be more energy efficient than millisecond or microsecond pulsed electric fields (msPEF or µsPEF); as nsPEF pulses are shorter, they generally consume less energy even when applied voltages are higher [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

A new mechanism for efficient hydrocarbon electro-extraction from Botryococcus braunii

Guionet

Hosseini

Teissié

et al. 2017

Biotechnol Biofuels

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BackgroundRecent understanding that specific algae have high hydrocarbon production potential has attracted considerable attention. Botryococcus braunii is a microalga with an extracellular hydrocarbon matrix, which makes it an appropriate green energy source.ResultsThis study focuses on extracting oil from the microalgae matrix rather than the cells, eliminating the need for an excessive electric field to create electro-permeabilization. In such a way, technical limitations due to high extraction energy and cost can be overcome. Here, nanosecond pulsed electric fields (nsPEF) with 80 ns duration and 20–65 kV/cm electric fields were applied. To understand the extraction mechanism, the structure of the algae was accurately studied under fluorescence microscope; extraction was quantified using image analysis; quality of extraction was examined by thin-layer chromatography (TLC); and the cell/matrix separation was observed real-time under a microscope during nsPEF application. Furthermore, optimization was carried out by screening values of electric fields, pulse repetition frequencies, and energy spent.ConclusionsThe results offer a novel method applicable for fast and continues hydrocarbon extraction process at low energy cost.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-017-0724-1) contains supplementary material, which is available to authorized users.

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E. coli electroeradication on a closed loop circuit by using milli-, micro- and nanosecond pulsed electric fields: Comparison between energy costs

Cited by 28 publications

References 37 publications

600-ns pulsed electric fields affect inactivation and antibiotic susceptibilities of Escherichia coli and Lactobacillus acidophilus

600-ns pulsed electric fields affect inactivation and antibiotic susceptibilities of Escherichia coli and Lactobacillus acidophilus

Oxidative modification and electrochemical inactivation of Escherichia coli upon cold atmospheric pressure plasma exposure

A new mechanism for efficient hydrocarbon electro-extraction from Botryococcus braunii

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