E. coli Alpha Hemolysin and Properties

Bakás, Laura Susana; Sabina, Maté; Romina, Vazquez; Herlax, Vanesa Silvana

doi:10.5772/33383

Cited by 4 publications

(3 citation statements)

References 166 publications

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“…Biofilm of UPEC provides a nutrient-rich environment which promotes growth and persistence of microorganisms at the site of infection, and protects bacteria from antimicrobial substances 1 , 14 . Moreover, UPEC strains often express and secrete a labile pore-forming toxin known as α- hemolysin production that is mainly associated with more virulent UPEC strains 15 , 16 .…”

Section: Introductionmentioning

confidence: 99%

Phenotypic Assays to Determine Virulence Factors of Uropathogenic Escherichia coli (UPEC) Isolates and their Correlation with Antibiotic Resistance Pattern

Tabasi

Karam

Habibi

et al. 2015

Osong Public Health and Research Perspectives

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ObjectivesUrinary tract infection caused by uropathogenic Escherichia coli (UPEC) strains is one of the most important infections in the world. UPEC encode widespread virulence factors closely related with pathogenesis of the bacteria. The purpose of this study was to evaluate the presence of different phenotypic virulence markers in UPEC isolates and determine their correlation with antibiotic resistance pattern.MethodsUPEC isolates from patients with different clinical symptoms of UTI were collected and screened for biofilm and hemolysin production, mannose resistant, and mannose sensitive hemagglutination (MRHA and MSHA, respectively). In addition, antimicrobial resistance pattern and ESBL-producing isolates were recorded.ResultsOf the 156 UPEC isolates, biofilm and hemolysin formation was seen in 133 (85.3%) and 53 (34%) isolates, respectively. Moreover, 98 (62.8%) and 58 (37.2%) isolates showed the presence of Types 1 fimbriae (MSHA) and P fimbriae (MRHA), respectively. Our results also showed a relationship between biofilm formation in UPEC isolated from acute cystitis patients and recurrent UTI cases. Occurrence of UTI was dramatically correlated with the patients' profiles. We observed that the difference in antimicrobial susceptibilities of the biofilm and nonbiofilm former isolates was statistically significant. The UPEC isolates showed the highest resistance to ampicillin, tetracycline, amoxicillin, and cotrimoxazole. Moreover, 26.9% of isolates were ESBL producers.ConclusionThis study indicated that there is a relationship between the phenotypic virulence traits of the UPEC isolates, patients' profiles, and antibiotic resistance. Detection of the phenotypic virulence factors could help to improve understanding of pathogenesis of UPEC isolates and better medical intervention.

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Section: Introductionmentioning

confidence: 99%

Phenotypic Assays to Determine Virulence Factors of Uropathogenic Escherichia coli (UPEC) Isolates and their Correlation with Antibiotic Resistance Pattern

Tabasi

Karam

Habibi

et al. 2015

Osong Public Health and Research Perspectives

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show abstract

“…However, as shown in Ref. 65 it is possible to include extra states in the optimization of the support functions in case that the user is interested in low-energy conduction states. Overall, we see that, thanks to the in-situ optimization of the support functions, the linear scaling version of BigDFT is able to correctly reproduce the electronic structure of a metallic system.…”

Section: Density Of Statesmentioning

confidence: 99%

Linear scaling DFT calculations for large tungsten systems using an optimized local basis

Mohr

Eixarch

Amsler

et al. 2018

Nuclear Materials and Energy

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Density Functional Theory (DFT) has become the quasi-standard for ab-initio simulations for a wide range of applications. While the intrinsic cubic scaling of DFT was for a long time limiting the accessible system size to some hundred atoms, the recent progress with respect to linear scaling DFT methods has allowed to tackle problems that are larger by many orders of magnitudes. However, as these linear scaling methods were developed for insulators, they cannot, in general, be straightforwardly applied to metals, as a finite temperature is needed to ensure locality of the density matrix. In this paper we show that, once finite electronic temperature is employed, the linear scaling version of the BigDFT code is able to exploit this locality to provide a computational treatment that scales linearly with respect to the number of atoms of a metallic system. We provide prototype examples based on bulk Tungsten, which plays a key role in finding safe and long-lasting materials for Fusion Reactors. We believe that such an approach might help in opening the path towards novel approaches for investigating the electronic structure of such materials, in particular when large supercells are required.

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“…Presence of clumping was taken as positive for haemagglutination. Controls were set up using nutrient broth without bacteria culture and washed blood group "O" red cells (25).…”

Section: Haemagglutination Testmentioning

confidence: 99%

Phenotypic Assay of Virulence Factors, Biofilms and Antibiotic Resistance among Enterobacterial Uropathogens from Cancer Patients

Adenipekun,

Lawal,

Nwaokorie

et al. 2018

JBMS

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Background: Virulence factors are associated with Enterobacterial uropathogens in cancer patients. Antimicrobial resistance in association with biofilm formation has intensified the pathogenicity of these uropathogens leading to high morbidity and therapeutic failures. Objective: This study determined and correlated the phenotypic virulence factors, antimicrobial resistance and biofilm forming Enterobacterial uropathogens in cancer patients. Materials and Methods: One hundred and seven (107) samples were collected randomly and processed between April and October, 2018. Twenty-five (25) isolates were recovered and characterized. Antimicrobial susceptibility testing against ten antimicrobials was performed using Kirby Bauer method. Extended spectrum beta-lactamase (ESBL) was determined with double disk synergy test. Virulence markers haemagglutinin was detected with haemagglutination test, haemolysin was detected by red cell haemolysis and biofilm formation was determined with the Congo red agar on all the isolates. Results: Out of the twenty-five (25) isolates, E. coli (72%), Klebsiella pneumoniae (24%) and Proteus mirabilis (4%) were isolated. 92% isolates were resistant to cefotaxime and 80% resistance to amoxicillin-clavulanic acid. 24% were positive for, ESBL, 4% for haemolysin test, 60% for haemagglutination and 40% for biofilm formation. From this study virulence markers were distributed among the isolates, however antibiotic resistance did not determine the presence or type of virulence markers. Conclusion: The present results may assist in developing strategic management of antibiotic therapy in cancer patients with urinary tract infections.

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E. coli Alpha Hemolysin and Properties

Cited by 4 publications

References 166 publications

Phenotypic Assays to Determine Virulence Factors of Uropathogenic Escherichia coli (UPEC) Isolates and their Correlation with Antibiotic Resistance Pattern

Phenotypic Assays to Determine Virulence Factors of Uropathogenic Escherichia coli (UPEC) Isolates and their Correlation with Antibiotic Resistance Pattern

Linear scaling DFT calculations for large tungsten systems using an optimized local basis

Phenotypic Assay of Virulence Factors, Biofilms and Antibiotic Resistance among Enterobacterial Uropathogens from Cancer Patients

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