E-cadherin junction formation involves an active kinetic nucleation process

Biswas, Kabir H.; Hartman, Kevin; Yu, Cheng-han; Harrison, O.J.; Song, Hang; Smith, Adam W.; Huang, William; Lin, Wan Chen; Guo, Zhenhuan; Padmanabhan, Anup; Troyanovsky, Sergey M.; Dustin, Michael L.; Shapiro, Lawrence; Honig, Barry; Zaidel-Bar, Ronen; Groves, Jay T.

doi:10.1073/pnas.1513775112

Cited by 90 publications

(120 citation statements)

References 78 publications

(92 reference statements)

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“…We first induced spatial localization of the nanoprobes, and hence E-cadherin, by placing the magnetic tweezer 2 μm above a target subcellular location (weak force mode). We observed a concentrated mCherry signals at the target subcellular region ( Figure 5A), consistent with the notion that E-cadherin clustering induces recruitment of F-actin at or adjacent to E-cadherin clusters ( Biswas et al, 2015 ;Engl et al, 2014 ;Lecuit and Yap, 2015 ).…”

Section: Spatial and Mechanical Responses Of E-cadherin Signalingsupporting

confidence: 89%

“…This approach to studying E-cadherin alters receptor diffusion and oligomerization and thus fails to fully recapitulate the mechanisms underlying E-cadherin junction formation in vivo. SLBs can resolve these issues and a recent study found that increased membrane viscosity is also critical for E-cadherin nucleation, clustering, and recruitment of junction complex proteins ( Biswas et al, 2015 ). However, the interplay between E-cadherin clustering and mechanical force application during E-cadherin junction formation, adapter recruitment, and cytoskeletal remodeling has yet to be elucidated.…”

Section: Identification Of Differential Roles Of Spatial Localizationmentioning

confidence: 99%

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A Mechanogenetic Toolkit for Interrogating Cell Signaling in Space and Time

Seo¹,

Southard²,

Kim³

et al. 2017

Cell

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SUMMARYTools capable of imaging and perturbing mechanical signaling pathways with fine spatiotemporal resolution have been elusive despite their importance in diverse cellular processes. The challenge in developing a mechanogenetic toolkit (i.e. selective and quantitative activation of genetically encoded mechanoreceptors) stems from the fact that many mechanically-activated processes are localized in space and time, yet additionally require mechanical loading to become activated. To Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. HHS Public Access

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Section: Spatial and Mechanical Responses Of E-cadherin Signalingsupporting

confidence: 89%

Section: Identification Of Differential Roles Of Spatial Localizationmentioning

confidence: 99%

A Mechanogenetic Toolkit for Interrogating Cell Signaling in Space and Time

Seo¹,

Southard²,

Kim³

et al. 2017

Cell

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“…E-cad cluster formation was found to be highly dependent on the viscous drag felt by aggregated ligands on the bilayer. On fluid bilayers, the clusters were unstable and transient (Biswas et al, 2015;Perez et al, 2005). In contrast, reduced diffusion of E-cad (by varying the bilayer fluidity or binding to the cytoskeleton) stabilized the cluster, strengthened the adhesion and redistributed the adhesive zones along the edge of the surrogate contact into a morphology that is reminiscent of the apical actin belt (Biswas et al, 2015).…”

Section: Ecm Sensingmentioning

confidence: 99%

How cells respond to environmental cues – insights from bio-functionalized substrates

Ruprecht

Monzo

Ravasio

et al. 2016

Journal of Cell Science

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Biomimetic materials have long been the (he)art of bioengineering. They usually aim at mimicking in vivo conditions to allow in vitro culture, differentiation and expansion of cells. The past decade has witnessed a considerable amount of progress in soft lithography, bioinspired micro-fabrication and biochemistry, allowing the design of sophisticated and physiologically relevant micro-and nanoenvironments. These systems now provide an exquisite toolbox with which we can control a large set of physicochemical environmental parameters that determine cell behavior. Biofunctionalized surfaces have evolved from simple protein-coated solid surfaces or cellular extracts into nano-textured 3D surfaces with controlled rheological and topographical properties. The mechanobiological molecular processes by which cells interact and sense their environment can now be unambiguously understood down to the single-molecule level. This Commentary highlights recent successful examples where bio-functionalized substrates have contributed in raising and answering new questions in the area of extracellular matrix sensing by cells, cell-cell adhesion and cell migration. The use, the availability, the impact and the challenges of such approaches in the field of biology are discussed.

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“…Yet, in some epithelia, contractile markers such as phosphorylated Myosin Light Chain (pMLC) are not highly enriched at junctional actin, particularly when peripheral thin bundles are clearly separable (Fig.1d). Although long, thick filament bundles are not apparent at junctional actin [44][45][46], a disordered array of short filaments can be contractile, particularly in the presence of cross-linkers such as -actinin 4 [47].…”

Section: Junctional Actinmentioning

confidence: 99%