E-cadherin expression pattern during zebrafish embryonic epidermis development

Sampedro, María Florencia; Izaguirre, María Fernanda; Sigot, Valeria

doi:10.12688/f1000research.15932.3

Cited by 4 publications

(3 citation statements)

References 39 publications

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“…For the segmentation of E-cadh-EGFP labelled cells; supervised machine learning was employed based on a pipeline previously reported [29] (figure S2). At each time point, z-projections and time-lapses that showed signs of stage or sample drift were registered using the manual drift correction plug-in in FIJI.…”

Section: Image Acquisition and Processingmentioning

confidence: 99%

Spatio-temporal analysis of collective migration in vivo by particle image velocimetry

et al. 2021

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Collective cell migration drives the formation of complex organ systems as well as certain tumour invasions and wound healing processes. A characteristic feature of many migrating collectives is tissue-scale polarity, whereby ‘leader’ cells at the tissue edge guide ‘followers’ cells that become assembled into polarized epithelial tissues. In this study, we employed particle image velocimetry (PIV) as a tool to quantitate local dynamics underlying the migration of the posterior lateral line primordium (pLLP) in zebrafish at a short time scale. Epithelial cadherin-EGFP was the fluorescent tracer in time-lapse images for PIV analysis. At the tissue level, global speed and directionality of the primordium were extracted from spatially averaged velocity fields. Interestingly, fluctuating velocity patterns evolve at the mesoscale level, which distinguishes the pseudo-mesenchymal leading front from the epithelialized trailing edge, and superimpose to the global deceleration of the whole primordium during the separation of a protoneuromast. Local velocity fields obtained by PIV proved sensitive to estimate the migration speed and directionality of the pLLP in zebrafish, predicting protoneuromast separation at short time scales. Finally, the PIV approach may be suitable for analysing the dynamics of other in vivo models of collective migration.

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Section: Image Acquisition and Processingmentioning

confidence: 99%

Spatio-temporal analysis of collective migration in vivo by particle image velocimetry

et al. 2021

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“…The embryonic epidermis is characterized by the expression of epithelial E-cadherin (E-cadh) outlining adherens junctions in both layers [30]. Based on the surface membrane labeling attained in fixed embryos with QDs, we analyzed whether the distribution of those nanoparticles correlates to that of E-cadh.…”

Section: Qds Label Cell-cell Contacts At the Evl But Not The Eblmentioning

confidence: 99%

Anionic Quantum Dots reveal actin-microridges in zebrafish epidermis

Sigot

Filho

Sampedro

et al. 2020

Methods Appl. Fluoresc.

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Enhancement of the aqueous solubility and functionalization of CdTe-QDs (Quantum Dots) via surface 28 modifications have made them suitable to be used as specific probes for cell imaging. Applications for 29 targeting cell surfaces have been widely demonstrated in vitro but their use in animal models is not 30 trivial. 31Here, we reported the interaction of mercaptosuccinic-coated (MSA) CdTe-QDs with the epidermis of 32 living and Carnoy-fixed zebrafish embryos. 33QDs concentrate along adherent junctions and reveal the characteristic pattern of actin microridges at 34 the apical surface of the enveloping layer.

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“…Cell-cell and cell-matrix adhesions are important regulators of cell shape dynamics [31]. Whereas increased cadherin based adhesion promotes polygonal cell morphology, its mis-regulation promotes cell-rounding [32][33][34]. Transient cell rounding is a common phenomenon in epithelial tissues under various physiological conditions.…”

Section: Introductionmentioning

confidence: 99%

Grhl3 promotes retention of epidermal cells under endocytic stress to maintain epidermal architecture in zebrafish

et al. 2021

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Epithelia such as epidermis cover large surfaces and are crucial for survival. Maintenance of tissue homeostasis by balancing cell proliferation, cell size, and cell extrusion ensures epidermal integrity. Although the mechanisms of cell extrusion are better understood, how epithelial cells that round up under developmental or perturbed genetic conditions are reintegrated in the epithelium to maintain homeostasis remains unclear. Here, we performed live imaging in zebrafish embryos to show that epidermal cells that round up due to membrane homeostasis defects in the absence of goosepimples/myosinVb (myoVb) function, are reintegrated into the epithelium. Transcriptome analysis and genetic interaction studies suggest that the transcription factor Grainyhead-like 3 (Grhl3) induces the retention of rounded cells by regulating E-cadherin levels. Moreover, Grhl3 facilitates the survival of MyoVb deficient embryos by regulating cell adhesion, cell retention and epidermal architecture. Our analyses have unraveled a mechanism of retention of rounded cells and its importance in epithelial homeostasis.

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E-cadherin expression pattern during zebrafish embryonic epidermis development

Cited by 4 publications

References 39 publications

Spatio-temporal analysis of collective migration in vivo by particle image velocimetry

Spatio-temporal analysis of collective migration in vivo by particle image velocimetry

Anionic Quantum Dots reveal actin-microridges in zebrafish epidermis

Grhl3 promotes retention of epidermal cells under endocytic stress to maintain epidermal architecture in zebrafish

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