Dysregulation of nitric oxide synthase activity and Bcl-2 and caspase-3 gene expressions in renal tissue of streptozotocin-induced diabetic rats

Aktuğ, Hüseyin; Çetintaş, Vildan Bozok; Kosova, Buket; Oltulu, Fatih; Demiray, Şirin Bakti; Çavuşoğlu, Türker; Akarca, Saadet Özen; Yavaşoğlu, Altuğ

doi:10.3906/sag-1104-38

Cited by 3 publications

(1 citation statement)

References 25 publications

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“…Additionally, hyperglycemia has been considered a critical mediator of altered lymphocyte function, which might drive the induction of oxidative stress besides play a key role in impairment of immunological responses following diabetes (10,11). Diabetes is a complicated metabolic disorder marked by dysfunction of the immune system as well as failure of the lymphoid organs, especially the spleen (12). Increased production of reactive oxygen species (ROS) can aggravate inflammation by activating NF-kB.…”

Section: Introductionmentioning

confidence: 99%

Okra [Abelmoschus esculentus (L.) Moench] improved blood glucose and restored histopathological alterations in splenic tissues in a rat model with streptozotocin-induced type 1 diabetes through CD8+ T cells and NF-kβ expression

Alblihd,

Alsharif,

Hamad

et al. 2023

Front. Vet. Sci.

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Diabetes mellitus is a complex metabolic syndrome that involves dysfunction of spleen and other lymphoid organs. Medicinal plants, including okra (Abelmoschus esculentus (L.) Moench), were used widely for diabetes treatment. Scarce data are available about the potential anti-diabetic effects of okra, the histopathological alterations in splenic tissues and the mechanistic pathways underlying this association. The current research investigated the effects of okra pod extract on the biochemical parameters and expression of CD8+ T cells and nuclear factor kappa (NF-k) B and releasing proinflammatory cytokines in spleen in streptozotocin (STZ)-induced diabetic rat models. A total of 50 mature male Wister albino rats were divided into five isolated groups; the first served as control (untreated) animals, the second (DM group) diabetes induced by STZ (at a dose of 45 mg/kg body weight, administered intraperitoneally), the third group (DM + Insulin): diabetic rats administered insulin subcutaneously (10 units/kg bw/day) daily for 4 weeks, the fourth group was administrated 400 mg/kg okra extract daily for 4 weeks, and diabetic induced rats in the fifth group were administrated 400 mg/kg okra extract daily for 4 weeks. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity in Abelmoschus esculentus (L.) Moench was studied, and the content of phenolic compounds in okra pods was estimated using high-performance liquid chromatography. Diabetes induction led to decreased body weight, increased blood glucose levels. Capsular thickness was significantly increased, white pulp was widely dispersed, and mature lymphocytes in the periphery were also drastically decreased, with thick follicular arteries, necrosis, and depletion of lymphocytes in the germinal center. Red pulp revealed severe congestion and degenerative changes, deposition of hemosiderin granules and lymphocytic depletion. In addition, collagen fiber deposition was increased also in this group. The induction of diabetes exaggerated NF-kβ expression and mediated downregulation of the expression of CD8+ T cells in spleen tissue. Interestingly, oral administration of okra extracts post diabetes induction could mitigate and reverse such adverse effects. Altogether, our study points out the potential benefits of okra in improving blood glucose levels and restoring histopathological alterations in splenic tissues through CD8+ T cells and NF-kβ expression in a diabetic rat model.

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Section: Introductionmentioning

confidence: 99%

Okra [Abelmoschus esculentus (L.) Moench] improved blood glucose and restored histopathological alterations in splenic tissues in a rat model with streptozotocin-induced type 1 diabetes through CD8+ T cells and NF-kβ expression

Alblihd,

Alsharif,

Hamad

et al. 2023

Front. Vet. Sci.

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Renal bcl-2/bax expression in childhood Henoch–Schönlein nephritis: prognostic importance?

Bek¹,

Oksal²,

Demircin³

et al. 2013

Turk J Med Sci

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Rabbit vesicular glands (gll. vesiculosae) are paired organs with variable size and shape. The ventral glandular surface is connected to the seminal ampullae (ampullae ductus deferentis). The cranial part from the dorsal glandular surface touches the colon and rectum. Its caudal part reaches the prostate, as reported by Kürtül et al. (1), Baron (2), Del Sol and Vásquez (3), Holtz and Foote (4), and McCracken et al. (5). Ultrasonographic and anatomic studies on the vesicular glands of the goat and Brazilian agouti made by Archana et al. (6) and Mollineau et al. (7) show that these glands are localized on the left and right sides of the urinary bladder (vesica urinaria) and laterally to the ductus deferens ampullae. There are few computed tomographic studies on animal vesicular glands. According to Zwicker et al. (8), squamous metaplasia, hyperplasia, and keratinization of the vesicular glands are found in 45% of New Zealand white rabbits. Transrectal ultrasonographic studies on the vesicular glands in the boar, stallion, deer, giraffe, and bull were carried out by Clark and Althouse (9), Goeritz et al. (10), Pozor (11), Gnemmi and Lefebvre (12), and Lueders et al. (13). These authors confirmed their relationship with

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Chronic moderate alcohol consumption induces iNOS expression in the penis: An immunohistochemical study

Gonca¹,

Yazır²,

Göçmez³

et al. 2014

Turk J Med Sci

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This study aimed to determine the physicochemical properties of the green tea powders produced from 2 different Turkish tea [Camellia sinensis (L.) O.Kuntze] clones (Derepazarı 7 and Fener) grown under different shade levels and harvested during 2 consecutive shooting periods. Moisture, ash, crude fiber, and total phenolic contents of the green tea powders were determined based on the experimental factors. Products were also evaluated for their water activity, bulk density, particle size, color, extraction yield, and antioxidant activity. The shooting period, specific to Turkish tea cultivation, showed remarkable effects on the main quality parameters of the green tea powders. It is noteworthy that the phenolic content and antioxidant activity of the products did not change significantly with treatments. The green tea powder produced from Derepazarı 7 clone, grown under dark shade and harvested in the first shooting period, was superior among the tested parameters.

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Dysregulation of nitric oxide synthase activity and Bcl-2 and caspase-3 gene expressions in renal tissue of streptozotocin-induced diabetic rats

Cited by 3 publications

References 25 publications

Okra [Abelmoschus esculentus (L.) Moench] improved blood glucose and restored histopathological alterations in splenic tissues in a rat model with streptozotocin-induced type 1 diabetes through CD8+ T cells and NF-kβ expression

Okra [Abelmoschus esculentus (L.) Moench] improved blood glucose and restored histopathological alterations in splenic tissues in a rat model with streptozotocin-induced type 1 diabetes through CD8+ T cells and NF-kβ expression

Renal bcl-2/bax expression in childhood Henoch–Schönlein nephritis: prognostic importance?

Chronic moderate alcohol consumption induces iNOS expression in the penis: An immunohistochemical study

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