Dysregulation of long non-coding RNA in breast cancer: an overview of mechanism and clinical implication

Ji, Wang; Ye, Chenyang; Xiong, Hanchu; Shen, Yong; Lu, Yi; Zhou, Jichun; Wang, Linbo

doi:10.18632/oncotarget.12537

Cited by 75 publications

(63 citation statements)

References 123 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Based on length, ncRNAs can be divided into short ncRNA (miRNA, siRNA and piRNA) and long ncRNA (lncRNA) (10). Aberrant expression of several lncRNAs has been reported in human diseases, including breast cancer (11,12). However, the number of lncRNAs that have been experimentally identified as oncogenes or tumor suppressors in breast cancer is considerably low (13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

Long non‑coding RNA SNHG1 promotes breast cancer progression by regulation of LMO4

Xiong

Feng

et al. 2020

Oncol Rep

View full text Add to dashboard Cite

Long non-coding RNA (lncRNA) small nucleolar RNA host gene 1 (SNHG1) was reported to be a critical regulator of tumorigenesis and is frequently deregulated in several cancer types. However, the exact mechanism by which SNHG1 contributes to breast cancer progression has not been fully elucidated. The identification of the molecular mechanism of SNHG1 is important for understanding the development of breast cancer and for improving the prognosis of the patients with this disease. In the present study, increased expression levels of SNHG1 were noted in breast cancer tumors following analysis of differentially expressed lncRNAs between 1,063 tumor and 102 normal tissues derived from The Cancer Genome Atlas Breast Invasive Carcinoma (TCGA-BRCA) dataset. This finding was further validated using 50 pairs of normal and tumor tissues that were collected from patients with breast cancer. Notably, SNHG1 expression was significantly correlated with estrogen receptor (ER)/progesterone receptor (PR) negative status (ER -/PR -) and advanced clinical stage in breast cancer tissues. Knockdown of SNHG1 led to cell growth arrest, cell cycle redistribution and cell migration inhibition of breast cancer cells. The miRDB database predicted that miR-573 interacts with SNHG1. RT-PCR confirmed the negative regulation of miR-573 levels by SNHG1 in breast cancer cells and the Dual-luciferase reporter assay confirmed their complementary binding. The repression of miR-573 by SNGH1 decreased LIM domain only 4 (LMO4) mRNA and protein expression levels in the breast cancer cell lines tested and induced the expression of cyclin D1 and cyclin E. In vitro experiments indicated that LMO4 overexpression could reverse siSNHG1-induced cell growth arrest, cell cycle redistribution and inhibition of cell migration in breast cancer cells. Moreover, the tumor xenograft model indicated that SNHG1 knockdown inhibited MDA-MB-231 growth in vivo and LMO4 overexpression reversed the tumor growth inhibition induced by SNHG1 knockdown. The present study demonstrated that SNHG1 acts as a novel oncogene in breast cancer via the SNHG/miR-573/LMO4 axis and that it could be a promising therapeutic target for patients with breast cancer.

show abstract

Section: Introductionmentioning

confidence: 99%

Long non‑coding RNA SNHG1 promotes breast cancer progression by regulation of LMO4

Xiong

Feng

et al. 2020

Oncol Rep

View full text Add to dashboard Cite

show abstract

“…For instance, miR-21, miR-124, miR-148a and miR-384 have been reported to be involved in the progression of HCC (17)(18)(19)(20). In recent years, emerging studies showed that aberrant expression of long noncoding RNAs (lncRNAs), a class of ncRNAs more than 200 nucleotides in length, was involved in the tumorigenesis and development of various cancer, including HCC (21)(22)(23)(24). Present research demonstrated that lncRNA function as competing endogenous RNAs (ceRNAs) or act as molecular 'sponges' to regulate the concentration, expression and biological functions of miRNAs (25).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-26a inhibits proliferation and metastasis of human hepatocellular carcinoma by regulating DNMT3B-MEG3 axis

Ren

Zhao

et al. 2017

Oncology Reports

View full text Add to dashboard Cite

miR-26a is known to play an important oncosuppressive role in HCC. However, its regulatory role and relationship with other non-coding RNAs is less clear. In the present study, we report that the expression levels of miR-26a and long non-coding RNA (lncRNA) maternally expressed gene 3 (MEG3) were frequently downregulated in HCC tissues compared to matched non-malignant tissues. In addition, the expression levels of miR-26a and MEG3 were negatively correlated with the tumor sizes and TNM clinical stage in HCC patients. Overexpression of miR-26a significantly reduced the capacity of proliferation, invasion and migration of HCC cells. Moreover, we demonstrated that DNA methyltransferase 3b (DNMT3B) was a direct target gene of miR-26a. Overexpression of miR-26a suppressed the expression level of DNMT3B. Inhibited expression of DNMT3B showed similar tumor suppressive effects induced by miR-26a upregulation, and resulted in the upregulation of MEG3. Furthermore, we found that the expression levels of DNMT3B were upregulated in the HCC tissues compared with non-malignant tissues, and it was inversely correlated with miR-26a and MEG3 in HCC tissues. Thus, these results provided a plausible link between the observed reduction of miR-26a and MEG3 in HCCs. Together, the present study added miR-26a/DNMT3B/MEG3 axis to the complex mechanisms of HCC development.

show abstract

“…Thus, there remains a considerable need for identification of novel biomarkers and better understanding of the molecular mechanisms underlying breast cancer. So far, several lncRNAs have been reported to show different expression patterns and be closely associated with breast cancer . For example, HOTAIR expression is increased in both primary breast tumours and metastases and can significantly predict subsequent metastasis and death of breast cancer .…”

Section: Discussionmentioning

confidence: 99%

The oncogenic potentials and diagnostic significance of long non‐coding RNA LINC00310 in breast cancer

Peng

et al. 2018

J Cellular Molecular Medi

View full text Add to dashboard Cite

Recent studies have revealed that long non‐coding RNAs (lncRNAs) are involved in different physiological processes and human diseases. However, to date, the function and overall clinical significance of the vast majority of lncRNAs in breast cancer remain largely unexplored. Here, we focused on LINC00310 by interrogating the breast invasive carcinoma data set of the Cancer Genome Atlas (TCGA). The results showed that LINC00310 was increased as breast cancer progressed, and the deregulation of LINC00310 was significantly associated with patients’ survival. Experiments with knockout (KO) approach by CRISPR/Cas9 system and the subsequent rescue experiments revealed that LINC00310 promoted cell proliferation by regulating c‐Myc expression in vitro. Nude mouse xenograft assay demonstrated that LINC00310 KO significantly suppressed tumour growth in vivo. Furthermore, we found that serum LINC00310 expression was significantly up‐regulated in patients with breast cancer, and receiver operating characteristic (ROC) curve analysis indicated that LINC00310 had a powerful capability of distinguishing patients with breast cancer from healthy individuals (the area under curve 0.828). Taken together, these results provide a more intuitive approach to explore the clinical relevance and functional roles of lncRNAs. As a result, lncRNAs, such as LINC00310, may be used in clinical applications as circulating markers for breast cancer.

show abstract

Dysregulation of long non-coding RNA in breast cancer: an overview of mechanism and clinical implication

Cited by 75 publications

References 123 publications

Long non‑coding RNA SNHG1 promotes breast cancer progression by regulation of LMO4

Long non‑coding RNA SNHG1 promotes breast cancer progression by regulation of LMO4

MicroRNA-26a inhibits proliferation and metastasis of human hepatocellular carcinoma by regulating DNMT3B-MEG3 axis

The oncogenic potentials and diagnostic significance of long non‐coding RNA LINC00310 in breast cancer

Contact Info

Product

Resources

About