Dysregulation of hepatic microRNA expression profiles with Clonorchis sinensis infection

Han, Seung-Beom; Tang, Qiaoran; Lu, Xi; Chen, Rui; Li, Yihong; Shu, Jiwu; Zhang, Xiaoli; Cao, Jianping

doi:10.1186/s12879-016-2058-1

Cited by 15 publications

(12 citation statements)

References 26 publications

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“…Because of the limited commercially available antibodies or probes, it is not appropriate for evaluating the pathogenesis or immune responses in hamsters ( Uddin et al, 2012 ). Although the rats develop resistance to reinfection by C. sinensis ( Quan et al, 2005 ), with the advantages of small size, low cost, wide source, easy feeding, and suitable for large-scale observation, rats have been broadly used as animal models for exploring pathogenesis of clonrochiasis ( Choi et al, 2004 ; Quan et al, 2005 ; Sohn et al, 2006 ; Han et al, 2016 , 2017 ). In addition, we could get better adults recovery rates and development of worms in rats models.…”

Section: Disscusionmentioning

confidence: 99%

“…Notably, C. sinensis has been classified as a Class I carcinogen by the International Agency for Research on Cancer ( Grosse et al, 2009 ). Our previous research had found that C. sinensis infection induced the dysregulation of hepatic microRNA and hepatic apoptosis in rat models ( Zhang et al, 2008 ; Han et al, 2016 ). However, the molecular pathogenesis of clonorchiasis is still not completely understood.…”

Section: Introductionmentioning

confidence: 99%

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Perturbations of Metabolomic Profiling of Spleen From Rats Infected With Clonorchis sinensis Determined by LC-MS/MS Method

Zhang

Chen

et al. 2020

Front. Mol. Biosci.

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Clonorchiasis is an important zoonotic parasitic disease worldwide. In view of the fact that parasite infection affects host metabolism, and there is an intricate relationship between metabolism and immunity. Metabolic analysis of the spleen could be helpful for understanding the pathophysiological mechanisms in clonorchiasis. A non-targeted ultra high performance liquid tandem chromatography quadrupole time of flight mass spectrometry (UHPLC-QTOF MS) approach was employed to investigate the metabolic profiles of spleen in rats at 4 and 8 weeks post infection with Clonorchis sinensis ( C. sinensis ). Then a targeted ultra-high performance liquid chromatography multiple reaction monitoring mass spectrometry (UHPLC-MRM-MS/MS) approach was used to further quantify amino acid metabolism. Multivariate data analysis methods, such as principal components analysis and orthogonal partial least squares discriminant analysis, were used to identify differential metabolites. Finally, a total of 396 and 242 significant differential metabolites were identified in ESI+ and ESI− modes, respectively. These metabolites included amino acids, nucleotides, carboxylic acids, lipids and carbohydrates. There were 38 significantly different metabolites shared in the two infected groups compared with the control group through the Venn diagram. The metabolic pathways analysis revealed that pyrimidine metabolism, aminoacyl-tRNA biosynthesis, purine metabolism and phenylalanine, tyrosine and tryptophan biosynthesis were significantly enriched in differential metabolites, which was speculated to be related to the disease progression of clonorchiasis. Furthermore, 15 amino acids screened using untargeted profiling can be accurately quantified and identifed by targeted metabolomics during clonrochiasis. These results preliminarily revealed the perturbations of spleen metabolism in clonorchiasis. Meanwhile, this present study supplied new insights into the molecular mechanisms of host-parasite interactions.

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Section: Disscusionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Perturbations of Metabolomic Profiling of Spleen From Rats Infected With Clonorchis sinensis Determined by LC-MS/MS Method

Zhang

Chen

et al. 2020

Front. Mol. Biosci.

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“…Tumor metastasis is a multi-step, multi-stage and multipathway complex process, which includes the detachment of tumor cells from the primary lesion, invasion into blood vessels or lymph vessels, migration and adhesion to appropriate sites, induction of tumor angiogenesis, anti-host antitumor immunity, and the eventual formation of distal metastasis (4). Multiple genes are involved during this process.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-26b inhibits the tumor growth of human liver cancer through the PI3K/Akt and NF-κB/MMP-9/VEGF pathways

Yan

Zhang

2018

Oncol Rep

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We investigated the role of microRNA-26b signaling in mediating the tumor growth of human liver cancer. MicroRNA-26b expression was observably downregulated in human liver cancer tissue. Forced upregulation of microRNA-26b had anticancer effects, inhibited cell proliferation, induced apoptosis and promoted Bax and caspase-3/-9 protein expression in a liver cancer cell line as determined by MTT assay, flow cytometry and western blot analysis. MicroRNA-26b significantly suppressed the PI3K/Akt and NF-κB/MMP-9/VEGF pathways. PI3K inhibitor significantly facilitated the effects of microRNA-26b regarding the suppression of the PI3K/Akt pathway, inhibition of cell proliferation, induction of apoptosis and increases in Bax and caspase-3/-9 protein expression in the liver cancer cell line. NF-κB inhibitor also significantly enhanced the effects of microRNA-26b regarding suppression of the NF-κB/MMP-9/VEGF pathway, inhibition of cell proliferation, induction of apoptosis, and promotion of Bax and caspase-3/-9 protein expression in the liver cancer cell line. The present study demonstrated that microRNA-26b inhibits the tumor growth of human liver cancer through the PI3K/Akt and NF-κB/MMP-9/VEGF pathways.

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“…In addition, C. sinensis has been classified by the International Agency for Research on Cancer (IARC) as a group 1 biocarcinogen in humans [ 3 ]. The main mechanism for hepatic and biliary damage is that the flukes migrate through the biliary system and they secrete or excrete metabolic products, which result in immunopathogenesis and simultaneous liver injury [ 4 , 5 ]. However, the mechanism underlying hepatic and biliary damages in hosts has yet to be fully elucidated.…”

Section: Introductionmentioning

confidence: 99%

Hepatic iron overload is associated with hepatocyte apoptosis during Clonorchis sinensis infection

et al. 2017

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BackgroundHepatic iron overload has been implicated in many liver diseases; however, whether it is involved in clonorchiasis remains unknown. The purpose of this study is to investigate whether Clonorchis sinensis (C. sinensis) infection causes hepatic iron overload, analyze the relationship between the iron overload and associated cell apoptosis, so as to determine the role of excess iron plays in C. sinensis-induced liver injury.MethodsThe Perls’ Prussian staining and atomic absorption spectrometry methods were used to investigate the iron overload in hepatic sections of wistar rats and patients infected with C. sinensis. The hepatic apoptosis was detected by transferase uridyl nick end labeling (TUNEL) methods. Spearman analysis was used for determining the correlation of the histological hepatic iron index and the apoptotic index.ResultsBlue iron particles were deposited mainly in the hepatocytes, Kupffer cells and endothelial cells, around the liver portal and central vein area of both patients and rats. The total iron score was found to be higher in the infected groups than the respective control from 8 weeks. The hepatic iron concentration was also significantly higher in treatment groups than in control rats from 8 weeks. The hepatocyte apoptosis was found to be significantly higher in the portal area of the liver tissue and around the central vein. However, spearman’s rank correlation coefficient revealed that there was a mildly negative correlation between the iron index and hepatocyte apoptosis.ConclusionsThis present study confirmed that hepatic iron overload was found during C. sinensis infection. This suggests that iron overload may be associated with hepatocyte apoptosis and involved in liver injury during C. sinensis infection. Further studies are needed to investigate the molecular mechanism involved here.

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Dysregulation of hepatic microRNA expression profiles with Clonorchis sinensis infection

Cited by 15 publications

References 26 publications

Perturbations of Metabolomic Profiling of Spleen From Rats Infected With Clonorchis sinensis Determined by LC-MS/MS Method

Perturbations of Metabolomic Profiling of Spleen From Rats Infected With Clonorchis sinensis Determined by LC-MS/MS Method

MicroRNA-26b inhibits the tumor growth of human liver cancer through the PI3K/Akt and NF-κB/MMP-9/VEGF pathways

Hepatic iron overload is associated with hepatocyte apoptosis during Clonorchis sinensis infection

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