Dysregulation of helper T lymphocytes in esophageal squamous cell carcinoma (ESCC) patients is highly associated with aberrant production of miR-21

Samiei, Hadiseh; Sadighi-Moghaddam, Bizhan; Mohammadi, Saeed; Gharavi, Abdolsamad; Abdolmaleki, Sara; Khosravi, Ayyoob; Kokhaei, Parviz; Bazzazi, Hadi; Memarian, Ali

doi:10.1007/s12026-019-09079-7

Cited by 19 publications

(24 citation statements)

References 53 publications

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“…Macrophage M1, regulatory T cells (Tregs), and T helper 1 cells (Th1) were significantly infiltrated into ESCC tissues, and their corresponding marker genes were observed highly expressed in ESCC ( Figure 1(b) ). Particularly, Tregs and Th1 were dysregulated and contributed to the initiation and propagation in ESCC [ 18 ]. The high infiltration of these cells showed that the functionalities of these immune cells might be impaired in ESCC.…”

Section: Discussionmentioning

confidence: 99%

[Retracted] The Functionalities and Clinical Significance of Tumor‐Infiltrating Immune Cells in Esophageal Squamous Cell Carcinoma

Zhang¹,

Wang²,

Wu³

et al. 2021

BioMed Research International

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Tumor-infiltrating immune cells have been implicated in the tumorigenesis and progression of esophageal squamous cell carcinoma (ESCC). However, the functionalities and clinical significance of immune cells remain largely unveiled. In this study, the gene expression data from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) were extracted. The relative infiltrating levels were estimated by single-sample gene set enrichment analysis. Some cytotoxic immune cells were attenuated, and resting cytotoxic immune cells were accumulated in ESCC. Remarkably, we also observed that infiltrating levels of macrophage M2 and resting natural killer (NK) cells were increased in nonresponders of CRT, and T cells that had anticancer activities such as activated memory CD4 and T helper 2 (Th2) cells were significantly reduced in ESCC tissues of the nonresponders. Moreover, the high infiltrations of the resting natural killer (NK) and dendritic cell (DC) were observed to result in a shorter overall survival in ESCC. Consistently, high expression of immune checkpoint genes, CTLA4 and HAVCR2, was associated with poor prognosis. Furthermore, STAT5B, a key transcription factor, as well as its target genes, involved in the regulation of T cells, was significantly downregulated in ESCC, especially subgroup I, indicating that downregulation of STAT5B might be associated with reduced T cell-mediated anticancer activity. In conclusion, the present study significantly improved our understanding of the regulatory roles of immune cells in ESCC.

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Section: Discussionmentioning

confidence: 99%

[Retracted] The Functionalities and Clinical Significance of Tumor‐Infiltrating Immune Cells in Esophageal Squamous Cell Carcinoma

Zhang¹,

Wang²,

Wu³

et al. 2021

BioMed Research International

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“…We realized that IL-10 and TNFα expressions were noticeably elevated among patients, while TGFβ expression was markedly decreased. In accordance with our findings, the elevation of IL-10 has been also reported in previous studies by Forsberg et al 33,34 and Manavalan et al 35 Moreover, several IL-10 polymorphisms have been associated with the susceptibility to CD, 36,37 which highlights the importance of this multifunctional cytokine 38 in the immunopathogenesis of CD. In order to better understand the role of IL-10 in the pathogenesis of CD, Hollon et al 39 revealed that gliadin increased the secretion of IL-10 from intestinal biopsy explants of CD patients and individuals with non-celiac gluten sensitivity.…”

Section: Discussionsupporting

confidence: 93%

The expansion of CD14+ CD163+ subpopulation of monocytes and myeloid cells‐associated cytokine imbalance; candidate diagnostic biomarkers for celiac disease (CD)

Babania

Mohammadi

Yaghoubi³

et al. 2021

Clinical Laboratory Analysis

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Celiac disease (CD) is a chronic autoimmune disorder of small intestine against dietary gluten, among genetically predisposed individuals. Monocytes are versatile innate immune cells involved in the regulation of inflammation, and strongly involved in the intestinal immunity. However, the role of monocytes and their subtypes in CD is not well demonstrated.Methods: Here, we assessed the polarization of CD14+ monocytes by evaluating the M1 (CD16) and M2 (CD163) markers by flowcytometry, their soluble forms (sCD16 and sCD163), and the serum levels of IL-10, IL-12, TGFβ, and TNFα cytokines using ELISA method, among 30 CD patients and 30 sex-and age-matched healthy subjects (HS). We also analyzed the diagnostic values of all variables with significant differences.Results: CD14+CD163+ monocytes were more frequent in CD patients than HS, while CD14+CD16+ monocytes were higher in HS. IL-10and TNFα increased, and TGFβ expression was decreased among CD patients. The sCD16 serum levels were elevated in patients, while sCD163 was higher but not significant among CD patients.CD163+/CD16+ and IL-10/IL-12 ratios were higher in CD patients, and TGFβ/TNFα ratio was higher in HS group. IL-10, CD14+CD163+, TNFα, and IL-10/IL-12 ratios with the AUC over 0.7 were introduced as fair diagnostic markers. Our findings revealed that the M2 (CD14+CD163+) monocytes were more frequent among CD patients, and the cytokine balance was disturbed. Conclusion:According to the significant functional diversities of monocyte subtypes between CD patients and HS group, these immunologic markers could be introduced as specific diagnostic biomarkers for CD.

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“…Previous studies have shown that the biology of the immune system is essential for the formation of complex tumor microenvironments [18] , including esophageal cancer [12] . Nowdays, it has been reported that a variety of biomarkers can affect the prognosis of patients with esophageal cancer by changing the immune microenvironment of the tumor, such as MiR-201, MiR-503 and PD-L1 [19][20][21] . PD-L1 has been proved to be the target of immunotherapy for patients with ESCC [22] .…”

Section: Discussionmentioning

confidence: 99%

Mining genes related to microenvironment of esophageal squamous cell carcinoma based on TCGA database

Liu¹,

Huang²,

Lin³

et al. 2021

Preprint

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Background：Esophageal squamous cell carcinoma (ESCC) is one of the most common malignant tumors in the world, and its mechanism is related to tumor microenvironment. Immune / stromal score can be used to identify differential expressed genes (DEGs) of cancer, but it has not been reported in ESCC.Methods: The expression profile and clinicopathological information of patients with ESCC were downloaded from The Cancer Genome Atlas (TCGA) database. The immune score and stromal score of patients with ESCC were calculated by ESTIMATE algorithm. The relationship between immune / stromal score and clinicopathological characteristics and survival prognosis of patients with ESCC was analyzed. DEGs were identified according to the score, followed by gene ontology (GO) and Kyoto Encyclopedia of Gene and Genome (KEGG) analysis. The DEGs with prognostic value were revealed by overall survival analysis. Protein-protein interaction (PPI) network analysis was performed on these DEGs. Finally, the DEGs were verified by Chinese Cancer Genomic Database-Esophageal Squamous-Cell Carcinoma (CCGD-ESCC) database. Results: Immune and stromal scores were related to the prognosis of patients with ESCC. 223 DEGs were screened according to immune / stromal scores. GO and KEGG analysis showed that the selected DEGs was mainly related to plasma membrne, immune response and inflammatory response. Overall survival analysis showed that 68 genes were associated with prognosis. PPI network analysis showed that the core genes were CD86, LCP2, TLR7, CSF1R, C1QA, IRF8, CTLA4 and so on. A total of 17 genes with prognostic value were verified by CCGD-ESCC database. Conclusions: we screened and verified tumor microenvironment-related genes that have prognostic value and are expected to be used as therapeutic targets for patients with ESCC.

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Dysregulation of helper T lymphocytes in esophageal squamous cell carcinoma (ESCC) patients is highly associated with aberrant production of miR-21

Cited by 19 publications

References 53 publications

[Retracted] The Functionalities and Clinical Significance of Tumor‐Infiltrating Immune Cells in Esophageal Squamous Cell Carcinoma

[Retracted] The Functionalities and Clinical Significance of Tumor‐Infiltrating Immune Cells in Esophageal Squamous Cell Carcinoma

The expansion of CD14+ CD163+ subpopulation of monocytes and myeloid cells‐associated cytokine imbalance; candidate diagnostic biomarkers for celiac disease (CD)

Mining genes related to microenvironment of esophageal squamous cell carcinoma based on TCGA database

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