Dysregulated miR-645 affects the proliferation and invasion of head and neck cancer cell

Sun, Qiang; Chen, Shuai; Zhao, Xue; Yan, Ming; Fang, Zheng; Wang, Haibin; Zhao, Junfang; Sun, Minglei; Han, Xiao; Chen, Wantao; Li, Xinming

doi:10.1186/s12935-015-0238-5

Cited by 11 publications

(8 citation statements)

References 35 publications

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“…It has been reported that miR-1246 increases the stemness and invasiveness of non-small cell lung cancer (Kim et al, 2016;Lin et al, 2019). miR-645 has been shown to be significantly associated with the poor prognosis of patients with head and neck cancer, and to promote head and neck cancer cell invasion and migration (Sun et al, 2015). In addition, the overexpression of miR-342-5p affects HER2 breast cancer cell motility and mitochondrial stability (Lindholm et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Identification of an 8-miRNA signature as a potential prognostic biomarker for glioma

Chen

Cai

et al. 2020

PeerJ

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Background Glioma is the most common form of primary malignant intracranial tumor. Methods In the current study, miRNA matrix were obtained from the Chinese Glioma Genome Atlas (CGGA), and then univariate Cox regression analysis and Lasso regression analysis were utilized to select candidate miRNAs and multivariate Cox regression analysis was applied to establish a miRNA signature for predicting overall survival (OS) of glioma. The signature was assessed with the area under the curve (AUC) of the receiver operating characteristic curve (ROC) and validated by data from Gene Expression Omnibus (GEO). Results Eight miRNAs (miR-1246, miR-148a, miR-150, miR-196a, miR-338-3p, miR-342-5p, miR-548h and miR-645) were included in the miRNA signature. The AUC of ROC analysis for 1- and 3-year OS in the CGGA dataset was 0.747 and 0.905, respectively. In the GEO dataset, The AUC for 1- and 3-year was 0.736 and 0.809, respectively. The AUC in both the CGGA and GEO datasets was similar to that based on WHO 2007 classification (0.736 and 0.799) and WHO 2016 classification (0.663 and 0.807). Additionally, Kaplan–Meier plot revealed that high-risk score patients had a poorer clinical outcome. Multivariate Cox regression analysis suggested that the miRNA signature was an independent prognosis-related factor [HR: 6.579, 95% CI [1.227−35.268], p = 0.028]. Conclusion On the whole, in the present study, based on eight miRNAs, a novel prognostic signature was developed for predicting the 1- and 3- year survival rate in glioma. The results may be conducive to predict the precise prognosis of glioma and to elucidate the underlying molecular mechanisms. However, further experimental researches of miRNAs are needed to validate the findings of this study.

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Section: Discussionmentioning

confidence: 99%

Identification of an 8-miRNA signature as a potential prognostic biomarker for glioma

Chen

Cai

et al. 2020

PeerJ

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“…MiR‐645 is one of these up‐regulated miRNAs. The up‐regulation of miR‐645 has been reported in colon cancer, adenocarcinoma, head and neck cancers …”

Section: Discussionmentioning

confidence: 99%

“…Similarly, the promotion effect of miR‐645 on cell metastasis has also been reported in renal clear cell carcinoma . Sun et al found high expression of miR‐645 in head and neck squamous cell carcinoma (HNSCC) with lymph node metastasis, and promotion effects of ectopic expression of miR‐645 on cell invasion and migration. Up to now, the function of miR‐645 in osteosarcoma has not been elucidated.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‐645 promotes metastasis of osteosarcoma via targeting tumor suppressor NM23 nucleoside diphosphate kinase 2

Jiao

Zhang

et al. 2018

Clin Exp Pharma Physio

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Osteosarcoma is the most common non-hematological primary bony malignancy in children and young adults with tumor metastasis being a common event at diagnosis. Understanding the pathogenesis of metastatic osteosarcoma may help identify potential therapeutic targets. In this study, we found that the level of microRNA-645 (miR-645) in osteosarcoma tumor tissues was significantly increased compared with their paired non-tumorous tissues, and was associated with histologic grade, TNM staging, lymph metastasis and distant metastasis. Knockdown of miR-645 caused a remarkable inhibition of migration of osteosarcoma U2OS cells. Furthermore, miR-645 inhibited NME2 (nucleoside diphosphate kinase 2) expression through directly binding to its 3' untranslated region. In human osteosarcoma tissues, we also found that NME2 was significantly decreased in tumor tissues, and its level was negatively correlated with miR-645. In addition, silencing NME2 attenuated the decreased cell migration by knockdown of miR-645, suggesting that it was involved in the miR-645 induced cell migration of osteosarcoma cells. Taken together, we found that miR-645 was up-regulated in osteosarcoma tissues and could promote osteosarcoma cell migration through directly inhibiting the tumor suppressor NME2. Our data provide novel insight into the role of miR-645 in osteosarcoma and indicate that miR-645 might be a potential therapeutic target of osteosarcoma.

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“…Overall survival of patients with advanced ovarian cancer or head or neck squamous cell carcinoma (HNSCC) is negatively associated with the miR-645 expression level, 31 , 32 suggesting that it may have a role in the pathogenesis of these malignancies. However, it was not demonstrated until recently that miR-645 has an effect on cancer cell proliferation, survival and resistance to treatment.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-645 is an oncogenic regulator in colon cancer

Guo

Wang

et al. 2017

Oncogenesis

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Despite advances in early diagnosis and the development of molecularly targeted therapy, curative treatment of colon cancer once it has metastasized is yet to be accomplished. This is closely associated with deregulated CRC cell proliferation and resistance to apoptosis. Here we reveal that upregulation of microRNA-645 (miR-645) through DNA copy number gain is responsible for enhanced proliferation and resistance to apoptosis in colon cancer. MiR-645 was upregulated in most colon cancer tissues related to adjacent normal mucosa. This appeared to be associated with amplification of a section of chromosome 20q13.13, where miR-645 is located. Inhibition of miR-645 reduced proliferation and enhanced sensitivity to apoptosis triggered by the chemotherapeutic drugs 5-fluorouracil and cisplatin in CRC cells, and retarded colon cancer xenograft growth. Conversely, overexpression of miR-645 in normal colon epithelial cells enhanced proliferation and triggered anchorage-independent cell growth. Although SRY-related HMG-box 30 (SOX30) was identified as a miR-645 target, its expression was only partially affected by miR-645, suggesting that miR-645 is a fine-tuning mechanism of SOX30 expression. Moreover, overexpression of SOX30 only moderately inhibited promotion of CRC cell proliferation by miR-645, indicating that miR-645 may have more targets that contribute to its pro-proliferation effect in colon cancer. Together, this study reveals that miR-645 can regulate oncogenesis in colon cancer with SOX30 being one of its targets.

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Dysregulated miR-645 affects the proliferation and invasion of head and neck cancer cell

Cited by 11 publications

References 35 publications

Identification of an 8-miRNA signature as a potential prognostic biomarker for glioma

Identification of an 8-miRNA signature as a potential prognostic biomarker for glioma

MicroRNA‐645 promotes metastasis of osteosarcoma via targeting tumor suppressor NM23 nucleoside diphosphate kinase 2

MicroRNA-645 is an oncogenic regulator in colon cancer

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