Dysregulated miR-27a-3p promotes nasopharyngeal carcinoma cell proliferation and migration by targeting Mapk10

Li, Lihua; Luo, Zhaohui

doi:10.3892/or.2017.5544

Cited by 48 publications

(35 citation statements)

References 35 publications

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“…MiR-27a excess was reported to act as an oncogene in the development of gastric and nasopharyngeal cancers 36 , 37 . However, there are still no published studies implicating that hepatic miR-27a promotes liver cancer development.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-27a regulates hepatic lipid metabolism and alleviates NAFLD via repressing FAS and SCD1

Zhang

Sun

Zhou

et al. 2017

Sci Rep

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MicroRNAs are implicated as crucial mediators in metabolic diseases including obesity, diabetes, and non-alcoholic fatty liver diseases (NAFLD). Here, we show miR-27a attenuated hepatic de novo lipogenesis and alleviated obesity-initiated NAFLD through inhibiting Fasn and Scd1 in liver. Hepatic levels of miR-27a were significantly augmented in HFD-fed and ob/ob mice. Further studies demonstrated that miR-27a directly interacted with 3′ untranslated region (3′-UTR) of hepatic Fasn and Scd1 mRNAs and reduced their expression levels in mice. Adenovirus-mediated overexpression of miR-27a robustly blocked sodium oleate-induced triglyceride (TG) accumulation in mouse primary hepatocytes and reduced liver TG contents in mice via repressing hepatic lipogenesis. Furthermore, ectopic expression of hepatic miR-27a impaired lipid contents of livers and attenuated NAFLD development through suppressing lipogenesis in HCD-fed and ob/ob mice. Together, our results reveal a critical role of miR-27a in lipid homeostasis of liver and pathogenesis of NAFLD.

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Section: Discussionmentioning

confidence: 99%

MicroRNA-27a regulates hepatic lipid metabolism and alleviates NAFLD via repressing FAS and SCD1

Zhang

Sun

Zhou

et al. 2017

Sci Rep

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“…Wang et al found that the dysregulation of miRNA-222 exerted an influence on the metastasis and invasion of thyroid cancer cells [12]. Li and Luo revealed the possible acceleration of nasopharyngeal carcinoma by miR-27a-3p overexpression [13]. The aberrant expression of miRNA will also influence the progression of HCC.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-490-3p suppresses hepatocellular carcinoma cell proliferation and migration by targeting the aurora kinase A gene (AURKA)

Zhang¹,

Bao²,

Zhao³

et al. 2020

aoms

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Introduction: Hepatocellular carcinoma (HCC) is the most common and prevalent cancer type among liver cancers. In this study, expression of miR-490-3p and aurora kinase A gene (AURKA) was investigated in HCC. Additionally, we explored the microRNA (miR)-490-3p/AURKA relationship as well as the influence on HCC cell proliferation and migration. Material and methods: The dual luciferase reporter assay serves to verify the target relationship between miR-490-3p and AURKA. miR-490-3p mimics, AURKA siRNA and AURKA cDNA, were transfected into HCC cells. Quantitative real-time polymerase chain reaction and western blot were chosen for examining the relative expression of miR-490-3p and AURKA in HCC tissues, adjacent tissues, HCC cells and normal cells. The study detected the proliferation of HCC cells with the application of MTT assay and colony formation assay. Transwell assay was applied for the observation of migration, and wound healing assay for invasion. Results: The experiment results showed that miR-490-3p expression was down-regulated and AURKA expression was up-regulated in HCC cells and tissues. AURKA was the target gene of miR-490-3p and overexpression of miR-490-3p could inhibit the expression of AURKA in HCC cells. miR-490-3p overexpression could inhibit HCC cell migration and invasion, while AURKA promoted HCC cell migration. All experiment results indicated that miR-490-3p was low-expressed while AURKA was over-expressed in HCC cells and tissues compared to normal liver cells and tissues. Conclusions: miR-490-3p could down-regulate the expression of AURKA, thus suppressing the proliferation and migration of HCC cells.

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“…[25,[32][33][34] Mir-27a-3p has been identi ed to promote tumor invasion and metastasis in gastric, colon, pancreatic, and nasopharyngeal cancers. [35][36][37][38] On the other hand, a tumor suppressor role has also been shown for miR-27a-3p in some cancers. For instance, it inhibits the proliferation of non-small cell lung cancer cells by targeting HOXB8.…”

Section: Discussionmentioning

confidence: 99%

Inverse Correlation of miR-27a-3p and CDH5 Expression Serves as a Diagnostic Biomarker of Proliferation and Metastasis of Clear Cell Renal Carcinoma

Wang

Zhou

Han

et al. 2020

Preprint

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Background: Cadherin-5 (CDH5) is aberrantly expressed in a variety of human cancers and plays an important role in angiogenesis. But its role in renal clear cell carcinoma (ccRCC) remains to be understood. Methods: The dysregulation of CDH5 expression in ccRCC and its association with clinicopathological characteristics were analyzed using the TCGA database. A meta-analysis was performed to verify the alteration of CDH5 expression in ccRCC using the GEO database. Quantitative RT-PCR and immunohistochemical staining were applied to assess the transcriptional and protein levels of CDH5. TargetScan and Tarbase were employed to predict the miRNAs with the potential to target mRNA of CDH5.Results: The mRNA level of CDH5 in ccRCC was significantly higher than in normal tissue. CDH5 mRNA expression could therefore serve as a potential diagnostic biomarker for ccRCC (AUC=0.844). However, the reduced CDH5 transcription levels were significantly correlated with patients in the T3-4 stage, lymph node and distant metastasis, as well as with a worse clinical outcome. We further observed that CDH5, at the protein level, was almost absent in ccRCC samples. In addition, a few databases screen showed that mir-27a-3p, is a highly conserved miRNA targeting CDH5. The expression of mir-27a-3p was significantly elevated in ccRCC tissues in contrast to normal tissues. Importantly, it was positively associated with the T3-4 stage and M stage, respectively, suggesting that the expression level of mir-27a-3p could serve as a diagnostic biomarker for ccRCC (AUC=0.775).Conclusion: Our data suggest that the reduced translational levels of CDH5 in ccRCC was related to the overexpression of mir-27a-3p. The higher mir-27a-3p and lower CDH5 expression significantly correlated with advanced clinical stages for ccRCC patients.

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Dysregulated miR-27a-3p promotes nasopharyngeal carcinoma cell proliferation and migration by targeting Mapk10

Cited by 48 publications

References 35 publications

MicroRNA-27a regulates hepatic lipid metabolism and alleviates NAFLD via repressing FAS and SCD1

MicroRNA-27a regulates hepatic lipid metabolism and alleviates NAFLD via repressing FAS and SCD1

MicroRNA-490-3p suppresses hepatocellular carcinoma cell proliferation and migration by targeting the aurora kinase A gene (AURKA)

Inverse Correlation of miR-27a-3p and CDH5 Expression Serves as a Diagnostic Biomarker of Proliferation and Metastasis of Clear Cell Renal Carcinoma

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Dysregulated miR-27a-3p promotes nasopharyngeal carcinoma cell proliferation and migration by targeting Mapk10

Cited by 48 publications

References 35 publications

MicroRNA-27a regulates hepatic lipid metabolism and alleviates NAFLD via repressing FAS and SCD1

MicroRNA-27a regulates hepatic lipid metabolism and alleviates NAFLD via repressing FAS and SCD1

MicroRNA-490-3p suppresses hepatocellular carcinoma cell proliferation and migration by targeting the aurora kinase A gene (AURKA)

Inverse Correlation of miR-27a-3p&nbsp;and CDH5&nbsp;Expression Serves as a Diagnostic Biomarker of Proliferation and Metastasis of Clear Cell Renal Carcinoma

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Inverse Correlation of miR-27a-3p and CDH5 Expression Serves as a Diagnostic Biomarker of Proliferation and Metastasis of Clear Cell Renal Carcinoma