Dysregulated circulating SOCS3 and HP expression associated with stable CAD and acute coronary syndrome: an integrated study based on bioinformatics analysis and case control validation

Abstract

Objective: To extensively use blood transcriptome analysis to identify potential diagnostic and therapeutic targets for cardiovascular diseases. Methods: Two gene expression datasets (GSE59867 and GSE62646) were downloaded from GEO DataSets to identify altered blood transcriptomes in patients with ST-segment elevation myocardial infarction (STEMI) compared to stable coronary artery disease (CAD). Thereafter, several computational approaches were taken to determine functional roles and regulatory networks of differentially expressed genes (DEGs). Finally, the expression of dysregulated two hub genes–suppressor of cytokine signaling 3 (SOCS3) and haptoglobin (HP)–were validated in a case–control study. Results: A total of 119 DEGs were identified in the discovery phase, consisting of 71 downregulated genes and 48 upregulated genes; two hub modules consisting of two hub genes–SOCS3 and HP–were identified. In the validation phase, both SOCS3 and HP were significantly downregulated in the stable CAD and acute coronary syndrome (ACS) patients when compared with healthy controls. Meanwhile, HP was significantly upregulated in STEMI patients when compared with stable CAD patients (p=0.041). Logistic regression analysis indicated that: downregulated expression of HP correlated with increased risk of CAD [odds ratio (OR)=0.52, 95% confidence interval (CI)=0.31~0.87, p=0.013]; and downregulated expression of SOCS3 correlated with increased risk of ACS (OR=0.66, 95% CI=0.46~0.94, p=0.023) when age, gender, history of hyperlipidemia, diabetes and hypertension were included as covariates. Conclusion: Future clarification of how SOCS3 and HP influence the pathogenesis of disease may pave the way for the development of novel diagnostic and therapeutic methods.

[Retracted] SOCS3 Gene Polymorphism and Hypertension Susceptibility in Chinese Population: A Two‐Center Case‐Control Study

“…They found that compared with healthy controls, patients with stable coronary artery disease (CAD) and acute coronary syndrome (ACS) had significantly lower levels of SOCS3 expression. And the probe expression levels of SOCS3 were significantly upregulated in patients with ST-segment elevation myocardial infarction (STEMI) when compared to stable CAD patients Zhang et al [ 31 ]. Ali et al used ELISA to detect SOCS3 levels in serum.…”

Identification of Potential Diagnostic Biomarkers From Circulating Cells During the Course of Sleep Deprivation-Related Myocardial Infarction Based on Bioinformatics Analyses

“…It is better to design study as in the previous literature. They collected peripheral blood samples from 302 patients for a case-control study to further confirm the bioinformatics analysis results based on the GSE59867 and GSE62646 datasets that dysregulated circulating hub genes expression were associated with MI development ( 60 ). Therefore, experiments using in vitro and in vivo models as well as prospective clinical studies will be indispensable for validation of the diagnostic and theragnostic value of these biomarkers.…”

The Search for Associations of Serum Proteins with the Presence of Unstable Atherosclerotic Plaque in Coronary Atherosclerosis

“…The level of haptoglobin significantly reduced in patients with stable coronary artery disease (CHD) and acute coronary syndrome (ACS) compared with healthy controls. Logistic regression analysis showed that a decrease in haptoglobin expression correlates with an increased risk of coronary heart disease [ 6 ]. Patients with acute myocardial infarction (MI) have elevated concentrations of alpha-1-acid glycoprotein [ 7 ].…”