Dysplastic features relevant to malignant transformation in atrophic epithelium of oral submucous fibrosis: A preliminary study

Sarode, Sachin C.; Chaudhary, Minal; Gadbail, Amol R.; Tekade, Satyajit Ashok; Patil, Shankargouda; Sarode, Gargi S

doi:10.1111/jop.12699

Cited by 25 publications

(24 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Follow‐up information was recorded for disease‐free survival of 3 years or more. Dysplastic features relevant to malignant transformation was carefully investigated histopathologically in atrophic epithelium of OSF under high‐power magnification . OSF cases showing basal cell layer hyperplasia were considered under LRED.…”

Section: Methodsmentioning

confidence: 99%

“…OSF cases showing basal cell layer hyperplasia were considered under LRED. OSF cases showing basal cell hyperplasia along with 2 or more other dysplastic features such as increased nuclear:cytoplasmic ratio, abnormal superficial mitosis and hyperchromasia were considered as HRED . OSF cases included in this study were taken from our previous published paper …”

Section: Methodsmentioning

confidence: 99%

“…Dysplastic features relevant to malignant transformation was carefully investigated histopathologically in atrophic epithelium of OSF under high-power magnification. 3 OSF cases showing basal cell layer hyperplasia were considered under LRED. OSF cases showing basal cell hyperplasia along with 2 or more other dysplastic features such as increased nuclear:cytoplasmic ratio, abnormal superficial mitosis and hyperchromasia were considered as HRED.…”

Section: Materials S and Me Thodsmentioning

confidence: 99%

“…in atrophic epithelium of OSF to evaluate high-risk epithelial dysplasia (HRED). 3 These findings are validated by evaluating the expression of Ki67, CD105 and α-smooth muscle actin (SMA) in atrophic epithelium of OSF. 4 However, there is dire need to explore the carcinogenesis-relevant molecular expressions in the disease progression model that are closely related to OSCC-SMF.…”

mentioning

confidence: 92%

“…Recently, it was observed that some of the malignant transformation‐relevant dysplastic features such as basal cell layer hyperplasia, abnormal superficial mitosis, increased nuclear size, increased nuclear:cytoplasmic ratio and hyperchromasia are especially useful in atrophic epithelium of OSF to evaluate high‐risk epithelial dysplasia (HRED) . These findings are validated by evaluating the expression of Ki67, CD105 and α‐smooth muscle actin (SMA) in atrophic epithelium of OSF .…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Ki67, CD105 and α‐smooth muscle actin expression in disease progression model of oral submucous fibrosis

Gadbail

Chaudhary

Sarode

et al. 2019

J of Invest & Clin Dent

Self Cite

View full text Add to dashboard Cite

Aim The aim of this study was to investigate the expression of Ki67, CD105 and α‐smooth muscle actin (α‐SMA) expression in oral submucous fibrosis (OSF) and oral squamous cell carcinoma in the background of OSF (OSCC‐SMF). Methods The study was carried out on paraffin‐embedded tissues of 30 normal oral mucosa (NOM), 50 OSF cases and 105 OSCC‐SMF. The immunohistochemistry was carried out to evaluate the expression of Ki67, CD105 and α‐SMA antigen. Results Ki67 labelling index (LI), CD105 and α‐SMA expression showed increasing trend from NOM, low‐risk epithelial dysplasia (LRED), high‐risk epithelial dysplasia (HRED), well‐differentiated squamous cell carcinoma (WDSCC), moderately differentiated squamous cell carcinoma to poorly differentiated squamous cell carcinoma. However, there was no significant difference of α‐SMA expression between HRED and WDSCC. In OSCC‐SMF, Ki67 LI, CD105 and α‐SMA were significantly higher in advanced clinical TNM stage, metastasis and less than 3 years patient survival as compared with early clinical TNM stage, non‐metastasis and 3 years or more patient survival. Conclusion Ki67 LI, α‐SMA and CD105 expression alone or together correspond with the disease progression model of SMF. Hence, expression of these markers can be used as a predictive marker of clinical outcome of OSCC‐SMF.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Materials S and Me Thodsmentioning

confidence: 99%

mentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Ki67, CD105 and α‐smooth muscle actin expression in disease progression model of oral submucous fibrosis

Gadbail

Chaudhary

Sarode

et al. 2019

J of Invest & Clin Dent

Self Cite

View full text Add to dashboard Cite

show abstract

DNA content abnormality in oral submucous fibrosis concomitant leukoplakia: A preliminary evaluation of the diagnostic and clinical implications

Xiao

et al. 2020

Diagnostic Cytopathology

View full text Add to dashboard Cite

Oral submucous fibrosis (OSF) is an insidious chronic oral and oropharyngeal potentially malignant disorder. Our previously studies reported that DNA image cytometry (ICM) using brushing added in the diagnosis of high-grade dysplasia and staging of oral leukoplakia (OLK). This preliminary study aimed to investigate the abnormal rate of DNA content in 30 cases of OSF including three cases of OSF concomitant OLK and evaluate their the diagnostic and clinical implications with special emphasis on the OSF concomitant OLK. Brushing and biopsy sample form each patient was processed by DNA-ICM and histological examination, respectively. The results of DNA-ICM analysis showed that all the lesions from the 27 patients with OSF only were identified as normal DNA content. Strikingly, two of three cases of OSF concomitant OLK were identified as abnormal DNA content, and one case of whom progressed to early oral squamous cell carcinoma at the follow-up of 31 months. Collectively, this preliminary evaluation revealed that DNA content abnormality was hardly observed in OSF only; however, it may be usually observed in OSF concomitant OLK, whom should be monitored on a priority basis for early detection of carcinoma. Multicenter large studies are needed to validate the findings of the current study.

show abstract

Oral Potentially Malignant Disorder Based Customized Epithelial Dysplasia Grading: a Need of the Hour

Raj¹,

Patil

Sarode

et al. 2018

Pathol. Oncol. Res.

View full text Add to dashboard Cite

Dysplastic features relevant to malignant transformation in atrophic epithelium of oral submucous fibrosis: A preliminary study

Abstract: Increased basal cell layer hyperplasia, abnormal superficial mitosis, increased nuclear-cytoplasmic ratio, and hyperchromasia are high-risk features for OSMF, and affected patients should be followed on a priority basis for the early detection of OSCC.

Cited by 25 publications

References 22 publications

Ki67, CD105 and α‐smooth muscle actin expression in disease progression model of oral submucous fibrosis

Ki67, CD105 and α‐smooth muscle actin expression in disease progression model of oral submucous fibrosis

DNA content abnormality in oral submucous fibrosis concomitant leukoplakia: A preliminary evaluation of the diagnostic and clinical implications

Oral Potentially Malignant Disorder Based Customized Epithelial Dysplasia Grading: a Need of the Hour

Contact Info

Product

Resources

About