Dyskerin Is a Component of the <i>Arabidopsis</i> Telomerase RNP Required for Telomere Maintenance

Kannan, K. P.; Nelson, Andrew D. L.; Shippen, Dorothy E.

doi:10.1128/mcb.01490-07

Cited by 72 publications

(72 citation statements)

References 51 publications

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“…1A). Putative H/ACA boxes are present in the 3′ ends of both TER1 and TER2, consistent with our previous finding that dyskerin is a component of Arabidopsis telomerase (33).…”

Section: Resultssupporting

confidence: 91%

RETRACTED: Two RNA subunits and POT1a are components of Arabidopsis telomerase

Cifuentes-Rojas

Kannan

Tseng

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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100

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Telomerase is a ribonucleoprotein (RNP) reverse transcriptase whose essential RNA subunit (TER) functions as a template for telomere repeat synthesis. Here we report the identification of two divergent TER moieties in the flowering plant Arabidopsis thaliana. Although both TER1 and TER2 copurify with telomerase activity and serve as templates for telomerase in vitro, depletion of TER1, but not TER2, leads to decreased telomerase activity and progressive telomere shortening in vivo. Moreover, mutation of the templating domain in TER1 results in the incorporation of mutant telomere repeats on chromosome ends. Thus, TER1 provides the major template for telomerase in vivo. We also show that POT1a binds TER1 with a Kd of 2 × 10 −7 M and the two components assemble into an enzymatically active RNP in vivo. In contrast, TER1-POT1b and TER2-POT1a associations were not observed. In other organisms POT1 proteins bind telomeric DNA and provide chromosome end protection. We propose that duplication of TER and POT1 in Arabidopsis fueled the evolution of novel proteinnucleic acid interactions and the migration of POT1 from the telomere to the telomerase RNP.OB fold | RNA template | gene duplication

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“…1A). Putative H/ACA boxes are present in the 3′ ends of both TER1 and TER2, consistent with our previous finding that dyskerin is a component of Arabidopsis telomerase (33).…”

Section: Resultssupporting

confidence: 91%

RETRACTED: Two RNA subunits and POT1a are components of Arabidopsis telomerase

Cifuentes-Rojas

Kannan

Tseng

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

100

View full text Add to dashboard Cite

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“…This is also displayed at elongated telomeres where, in the presence of telomerase, telomeres shorten at a constant rate until they reach the point of equilibrium where the rate of degradation decreases. It can also be in Arabidopsis that, when shuffling multiple alleles that control telomere length homeostasis, drastic changes in telomere length are seen as a consequence of telomerase Kannan et al 2008;STEP1, Kwon and Chung 2004;WHY1, Yoo et al 2007;and TAC1, Ren et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Genetic Architecture of Natural Variation of Telomere Length inArabidopsis thaliana

et al. 2014

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Telomeres represent the repetitive sequences that cap chromosome ends and are essential for their protection. Telomere length is known to be highly heritable and is derived from a homeostatic balance between telomeric lengthening and shortening activities. Specific loci that form the genetic framework underlying telomere length homeostasis, however, are not well understood. To investigate the extent of natural variation of telomere length in Arabidopsis thaliana, we examined 229 worldwide accessions by terminal restriction fragment analysis. The results showed a wide range of telomere lengths that are specific to individual accessions. To identify loci that are responsible for this variation, we adopted a quantitative trait loci (QTL) mapping approach with multiple recombinant inbred line (RIL) populations. A doubled haploid RIL population was first produced using centromere-mediated genome elimination between accessions with long (Pro-0) and intermediate (Col-0) telomere lengths. Composite interval mapping analysis of this population along with two established RIL populations (Ler-2/Cvi-0 and Est-1/Col-0) revealed a number of shared and unique QTL. QTL detected in the Ler-2/Cvi-0 population were examined using near isogenic lines that confirmed causative regions on chromosomes 1 and 2. In conclusion, this work describes the extent of natural variation of telomere length in A. thaliana, identifies a network of QTL that influence telomere length homeostasis, examines telomere length dynamics in plants with hybrid backgrounds, and shows the effects of two identified regions on telomere length regulation.

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“…Most of the genes in the largest submodule (M63.1, size = 358) were connected to pseudouridine synthase NAP57, Whirly family protein ATWHY2, HISTONE DEACETYLASE A3 (HDA3), and HISTONE DEACETYLASE 2C (HD2C). NAP57 is a component of telomerase ribonucleoprotein and is required for telomere maintenance (Kannan et al, 2008). ATWHY2 is a mitochondrial protein that is involved in organelle genome repair (Cappadocia et al, 2010).…”

Section: Module M63 Suggests a Potential Role Of Chromatin Dynamics Imentioning

confidence: 99%

Revealing Shared and Distinct Gene Network Organization in Arabidopsis Immune Responses by Integrative Analysis

et al. 2015

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Pattern-triggered immunity (PTI) and effector-triggered immunity (ETI) are two main plant immune responses to counter pathogen invasion. Genome-wide gene network organizing principles leading to quantitative differences between PTI and ETI have remained elusive. We combined an advanced machine learning method and modular network analysis to systematically characterize the organizing principles of Arabidopsis (Arabidopsis thaliana) PTI and ETI at three network resolutions. At the single network node/ edge level, we ranked genes and gene interactions based on their ability to distinguish immune response from normal growth and successfully identified many immune-related genes associated with PTI and ETI. Topological analysis revealed that the top-ranked gene interactions tend to link network modules. At the subnetwork level, we identified a subnetwork shared by PTI and ETI encompassing 1,159 genes and 1,289 interactions. This subnetwork is enriched in interactions linking network modules and is also a hotspot of attack by pathogen effectors. The subnetwork likely represents a core component in the coordination of multiple biological processes to favor defense over development. Finally, we constructed modular network models for PTI and ETI to explain the quantitative differences in the global network architecture. Our results indicate that the defense modules in ETI are organized into relatively independent structures, explaining the robustness of ETI to genetic mutations and effector attacks. Taken together, the multiscale comparisons of PTI and ETI provide a systems biology perspective on plant immunity and emphasize coordination among network modules to establish a robust immune response.

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Dyskerin Is a Component of the Arabidopsis Telomerase RNP Required for Telomere Maintenance

Cited by 72 publications

References 51 publications

RETRACTED: Two RNA subunits and POT1a are components of Arabidopsis telomerase

RETRACTED: Two RNA subunits and POT1a are components of Arabidopsis telomerase

Genetic Architecture of Natural Variation of Telomere Length inArabidopsis thaliana

Revealing Shared and Distinct Gene Network Organization in Arabidopsis Immune Responses by Integrative Analysis

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