Dysfunctional voiding behavior and impaired muscle contractility in a rat model of detrusor underactivity

Gonzalez, Eric J.; Odom, Michael; Hannan, Johanna L.; Grill, Warren M.

doi:10.1002/nau.24777

Cited by 5 publications

(2 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Metabolic syndrome and OAB may share common pathophysiology mechanisms [ 39 ] since patients with metabolic syndrome exhibit a higher incidence of OAB [ 40 ]. Impaired purinergic signaling in the bladder has been demonstrated in animal models of diabetes, dyslipidemia, obesity and hypertension [ 41 , 42 , 43 , 44 ] and, therefore, ATP and other purines might be involved in the development of voiding dysfunctions associated with systemic diseases, including the metabolic syndrome.…”

Section: Introductionmentioning

confidence: 99%

Urinary ATP Levels Are Controlled by Nucleotidases Released from the Urothelium in a Regulated Manner

et al. 2022

View full text Add to dashboard Cite

Adenosine 5′-triphosphate (ATP) is released in the bladder lumen during filling. Urothelial ATP is presumed to regulate bladder excitability. Urinary ATP is suggested as a urinary biomarker of bladder dysfunctions since ATP is increased in the urine of patients with overactive bladder, interstitial cystitis or bladder pain syndrome. Altered urinary ATP might also be associated with voiding dysfunctions linked to disease states associated with metabolic syndrome. Extracellular ATP levels are determined by ATP release and ATP hydrolysis by membrane-bound and soluble nucleotidases (s-NTDs). It is currently unknown whether s-NTDs regulate urinary ATP. Using etheno-ATP substrate and HPLC-FLD detection techniques, we found that s-NTDs are released in the lumen of ex vivo mouse detrusor-free bladders. Capillary immunoelectrophoresis by ProteinSimple Wes determined that intraluminal solutions (ILS) collected at the end of filling contain ENTPD3 > ENPP1 > ENPP3 ≥ ENTPD2 = NT5E = ALPL/TNAP. Activation of adenylyl cyclase with forskolin increased luminal s-NTDs release whereas the AC inhibitor SQ22536 had no effect. In contrast, forskolin reduced and SQ22536 increased s-NTDs release in the lamina propria. Adenosine enhanced s-NTDs release and accelerated ATP hydrolysis in ILS and lamina propria. Therefore, there is a regulated release of s-NTDs in the bladder lumen during filling. Aberrant release or functions of urothelial s-NTDs might cause elevated urinary ATP in conditions with abnormal bladder excitability.

show abstract

Section: Introductionmentioning

confidence: 99%

Urinary ATP Levels Are Controlled by Nucleotidases Released from the Urothelium in a Regulated Manner

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Dr. Grill’s talk focused on the clinical disorder of bladder under-activity and the role of nervous system control, specifically the cholinergic system, in this vital physiological function. Patients and preclinical models with bladder under-activity have reduced voiding pressures and efficiency, which can be treated with neurostimulation of peripheral nerves (Gonzalez et al 2021 ; Hokanson et al 2021 ). Loss of control over voiding, for example in spinal cord injury, significantly impacts patient autonomy and quality of life.…”

Section: Scientific Sessionsmentioning

confidence: 99%

The Fifth Bioelectronic Medicine Summit: today’s tools, tomorrow’s therapies

Chang,

Gabalski,

Huerta

et al. 2023

Bioelectron Med

Self Cite

View full text Add to dashboard Cite

The emerging field of bioelectronic medicine (BEM) is poised to make a significant impact on the treatment of several neurological and inflammatory disorders. With several BEM therapies being recently approved for clinical use and others in late-phase clinical trials, the 2022 BEM summit was a timely scientific meeting convening a wide range of experts to discuss the latest developments in the field. The BEM Summit was held over two days in New York with more than thirty-five invited speakers and panelists comprised of researchers and experts from both academia and industry. The goal of the meeting was to bring international leaders together to discuss advances and cultivate collaborations in this emerging field that incorporates aspects of neuroscience, physiology, molecular medicine, engineering, and technology. This Meeting Report recaps the latest findings discussed at the Meeting and summarizes the main developments in this rapidly advancing interdisciplinary field. Our hope is that this Meeting Report will encourage researchers from academia and industry to push the field forward and generate new multidisciplinary collaborations that will form the basis of new discoveries that we can discuss at the next BEM Summit.

show abstract

Effects of different diets used to induce obesity/metabolic syndrome on bladder function in rats

Wang

Maher

et al. 2023

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

Preclinical and human studies on the relationship between obesity/metabolic syndrome (MetS) and lower urinary tract dysfunction (LUTD) are inconsistent. We compared the temporal effects of feeding four different diets used to induce obesity/MetS, including 60% fructose, 2% cholesterol 10% lard, 30% fructose 20% lard, or 32.5% lard diet, up to 42 weeks, on metabolic parameters and bladder function in male Sprague-Dawley rats. Rats fed a 30% fructose 20% lard or 32.5% lard diet consumed less food (grams), but only the 32.5% lard diet group took in more calories. Feeding rats a 60% fructose or 30% fructose 20% lard diet led to glucose intolerance and increased blood pressure. Higher body weight and increased cholesterol levels were observed in the rats maintained on a 2% cholesterol 10% lard diet, whereas exposure to a 32.5% lard diet affected most of the above parameters. Voiding behavior measurement showed that voiding frequency and the total voided volume were lower in the experimental diet groups except for the 30% fructose 20% lard group. The mean voided volume was lower in the 30% fructose 20% lard and 32.5% lard groups compared to the control group. Cystometric analysis revealed a decreased bladder capacity, mean voided volume, inter-micturition interval, and compliance in the 32.5% lard diet group. In conclusion, experimental diets including 60% fructose, 30% fructose 20% lard, or 2% cholesterol 10% lard diet differently affected physiological and metabolic parameters and bladder function to a limited extent, while exposure to a 32.5% lard diet had a greater impact.

show abstract

Dysfunctional voiding behavior and impaired muscle contractility in a rat model of detrusor underactivity

Cited by 5 publications

References 24 publications

Urinary ATP Levels Are Controlled by Nucleotidases Released from the Urothelium in a Regulated Manner

Urinary ATP Levels Are Controlled by Nucleotidases Released from the Urothelium in a Regulated Manner

The Fifth Bioelectronic Medicine Summit: today’s tools, tomorrow’s therapies

Effects of different diets used to induce obesity/metabolic syndrome on bladder function in rats

Contact Info

Product

Resources

About